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Singh V, Amunts A
Methods in molecular biology (Clifton, N.J.) 2192 (-) 197-210 [2020-11-25; online 2020-11-25]
Mitochondrial ribosomes (mitoribosomes) are specialized machineries that carry out the synthesis of a limited number of proteins encoded in the mitochondrial genome, including components of the oxidative phosphorylation pathway. They have incorporated several structural features distinguishing them from bacterial and eukaryotic cytosolic counterparts. Our current understanding of the assembly and functioning of mitoribosomes is limited, and recent developments in cryo-EM provide promising directions for detailed investigation. Here we describe methods to purify mitoribosomes from human embryonic kidney cells for cryo-EM studies.
PubMed 33230775
DOI 10.1007/978-1-0716-0834-0_15
Crossref 10.1007/978-1-0716-0834-0_15