The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications.

Nilsson RH, Larsson KH, Taylor AFS, Bengtsson-Palme J, Jeppesen TS, Schigel D, Kennedy P, Picard K, Glöckner FO, Tedersoo L, Saar I, Kõljalg U, Abarenkov K

Nucleic Acids Res. 47 (D1) D259-D264 [2019-01-08; online 2018-10-30]

UNITE ( is a web-based database and sequence management environment for the molecular identification of fungi. It targets the formal fungal barcode-the nuclear ribosomal internal transcribed spacer (ITS) region-and offers all ∼1 000 000 public fungal ITS sequences for reference. These are clustered into ∼459 000 species hypotheses and assigned digital object identifiers (DOIs) to promote unambiguous reference across studies. In-house and web-based third-party sequence curation and annotation have resulted in more than 275 000 improvements to the data over the past 15 years. UNITE serves as a data provider for a range of metabarcoding software pipelines and regularly exchanges data with all major fungal sequence databases and other community resources. Recent improvements include redesigned handling of unclassifiable species hypotheses, integration with the taxonomic backbone of the Global Biodiversity Information Facility, and support for an unlimited number of parallel taxonomic classification systems.

DDLS Fellow

Johan Bengtsson-Palme

PubMed 30371820

DOI 10.1093/nar/gky1022

Crossref 10.1093/nar/gky1022

pii: 5146189
pmc: PMC6324048

Publications 9.5.0