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Dietvorst J, Ferrer-Vilanova A, Iyengar SN, Russom A, Vigués N, Mas J, Vilaplana L, Marco MP, Guirado G, Muñoz-Berbel X
Anal. Chem. 94 (2) 787-792 [2022-01-18; online 2021-12-21]
The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nanostructure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases.
PubMed 34931815
DOI 10.1021/acs.analchem.1c03326
Crossref 10.1021/acs.analchem.1c03326