{"entity": "publication", "iuid": "1d9cf1c517064ab9a98a64a520f04cd1", "timestamp": "2026-08-22T06:56:58.013Z", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9cf1c517064ab9a98a64a520f04cd1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9cf1c517064ab9a98a64a520f04cd1"}}, "title": "Bacteria Detection at a Single-Cell Level through a Cyanotype-Based Photochemical Reaction.", "authors": [{"family": "Dietvorst", "given": "Jiri", "initials": "J"}, {"family": "Ferrer-Vilanova", "given": "Amparo", "initials": "A", "orcid": "0000-0002-4223-8442", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/05062539a66e4dbcac8685968c9afbde.json"}}, {"family": "Iyengar", "given": "Sharath Narayana", "initials": "SN", "orcid": "0000-0001-5348-3526", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b3ab95c0e76c402c85016a02822b247b.json"}}, {"family": "Russom", "given": "Aman", "initials": "A"}, {"family": "Vigu\u00e9s", "given": "N\u00faria", "initials": "N"}, {"family": "Mas", "given": "Jordi", "initials": "J"}, {"family": "Vilaplana", "given": "Llu\u00efsa", "initials": "L"}, {"family": "Marco", "given": "Maria-Pilar", "initials": "MP", "orcid": "0000-0002-4064-1668", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1b81a388946c463594c97e0d56eccdd5.json"}}, {"family": "Guirado", "given": "Gonzalo", "initials": "G", "orcid": "0000-0003-2128-7007", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9482a8c5b9f045f9bef160bf01f86082.json"}}, {"family": "Mu\u00f1oz-Berbel", "given": "Xavier", "initials": "X", "orcid": "0000-0002-6447-5756", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fb45e97411914b24965ccb787cb9c421.json"}}], "type": "journal article", "published": "2022-01-18", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "94", "issue": "2", "pages": "787-792", "issn-l": "0003-2700"}, "abstract": "The detection of living organisms at very low concentrations is necessary for the early diagnosis of bacterial infections, but it is still challenging as there is a need for signal amplification. Cell culture, nucleic acid amplification, or nanostructure-based signal enhancement are the most common amplification methods, relying on long, tedious, complex, or expensive procedures. Here, we present a cyanotype-based photochemical amplification reaction enabling the detection of low bacterial concentrations up to a single-cell level. Photocatalysis is induced with visible light and requires bacterial metabolism of iron-based compounds to produce Prussian Blue. Bacterial activity is thus detected through the formation of an observable blue precipitate within 3 h of the reaction, which corresponds to the concentration of living organisms. The short time-to-result and simplicity of the reaction are expected to strongly impact the clinical diagnosis of infectious diseases.", "doi": "10.1021/acs.analchem.1c03326", "pmid": "34931815", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8771638"}], "notes": [], "created": "2026-08-20T08:08:35.694Z", "modified": "2026-08-20T08:08:35.884Z"}