Probing the activity of a recombinant Zn

Ravishankar H, Barth A, Andersson M

Biopolymers 109 (2) - [2018-02-00; online 2017-11-23]

P-type ATPase proteins maintain cellular homeostasis and uphold critical concentration gradients by ATP-driven ion transport across biological membranes. Characterization of single-cycle dynamics by time-resolved X-ray scattering techniques in solution could resolve structural intermediates not amendable to for example crystallization or cryo-electron microscopy sample preparation. To pave way for such time-resolved experiments, we used biochemical activity measurements, Attenuated Total Reflectance (ATR) and time-dependent Fourier-Transform Infra-Red (FTIR) spectroscopy to identify optimal conditions for activating a Zn

Affiliated researcher

PubMed 29168553

DOI 10.1002/bip.23087

Crossref 10.1002/bip.23087

Publications 9.5.0