Kinetics of dCas9 target search in

Jones DL, Leroy P, Unoson C, Fange D, Ćurić V, Lawson MJ, Elf J

Science 357 (6358) 1420-1424 [2017-09-29; online 2017-09-28]

How fast can a cell locate a specific chromosomal DNA sequence specified by a single-stranded oligonucleotide? To address this question, we investigate the intracellular search processes of the Cas9 protein, which can be programmed by a guide RNA to bind essentially any DNA sequence. This targeting flexibility requires Cas9 to unwind the DNA double helix to test for correct base pairing to the guide RNA. Here we study the search mechanisms of the catalytically inactive Cas9 (dCas9) in living

Affiliated researcher

PubMed 28963258

DOI 10.1126/science.aah7084

Crossref 10.1126/science.aah7084

pii: 357/6358/1420
pmc: PMC6150439
mid: EMS79094

Publications 9.5.0