Controlling adsorption of albumin with hyaluronan on silica surfaces and sulfonated latex particles.
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Berts I, Fragneto G, Porcar L, Hellsing MS, Rennie AR
J Colloid Interface Sci 504 (-) 315-324 [2017-10-15; online 2017-05-17]
Polysaccharides are known to modify binding of proteins at interfaces and this paper describes studies of these interactions and how they are modified by pH. Specifically, the adsorption of human serum albumin on to polystyrene latex and to silica is described, focusing on how this is affected by hyaluronan. Experiments were designed to test how such binding might be modified under relevant physiological conditions. Changes in adsorption of albumin alone and the co-adsorption of albumin and hyaluronan are driven by electrostatic interactions. Multilayer binding is found to be regulated by the pH of the solution and the molecular mass and concentration of hyaluronan. Highest adsorption was observed at pH below 4.8 and for low molecular mass hyaluronan (≤150kDa) at concentrations above 2mgml
PubMed 28551526
DOI 10.1016/j.jcis.2017.05.037
Crossref 10.1016/j.jcis.2017.05.037
pii: S0021-9797(17)30557-X