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Wennmalm S
Methods 140-141 (-) 23-31 [2018-05-01; online 2018-02-02]
Inverse Fluorescence Correlation Spectroscopy (iFCS) is a variant of FCS where unlabeled particles in solution, or domains in membranes, displace their surrounding, signal-generating molecules and thereby generate fluctuations. iFCS has to date been applied to unlabeled as well as labeled particles and protein molecules, using fluorescence as well as Raman scattering as a signal source, in diffraction-limited detection volumes as well as in nano-wells, and on fixed surfaces as well as in lipid bilayers. This review describes these applications and discusses the potentials and pitfalls when using iFCS.
PubMed 29397309
DOI 10.1016/j.ymeth.2018.01.005
Crossref 10.1016/j.ymeth.2018.01.005
pii: S1046-2023(17)30264-5