Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.

Chaker-Margot M, Hunziker M, Barandun J, Dill BD, Klinge S

Nat. Struct. Mol. Biol. 22 (11) 920-923 [2015-11-00; online 2015-10-19]

Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae as a model organism, we developed a system that recapitulates and arrests ribosome assembly at early stages, thus providing in vivo snapshots of nascent preribosomal particles. Here we report the stage-specific order in which 70 ribosome-assembly factors associate with preribosomal RNA domains, thereby forming the 6-MDa small-subunit processome.

Jonas Barandun

SciLifeLab Fellow

PubMed 26479197

DOI 10.1038/nsmb.3111

Crossref 10.1038/nsmb.3111

pii: nsmb.3111

Publications 9.5.0