A practical guide to build

Montero-Mendieta S, Grabherr M, Lantz H, De la Riva I, Leonard JA, Webster MT, VilĂ  C

PeerJ 5 (-) e3702 [2017-09-01; online 2017-09-01]

Whole genome sequencing (WGS) is a very valuable resource to understand the evolutionary history of poorly known species. However, in organisms with large genomes, as most amphibians, WGS is still excessively challenging and transcriptome sequencing (RNA-seq) represents a cost-effective tool to explore genome-wide variability. Non-model organisms do not usually have a reference genome and the transcriptome must be assembled

Affiliated researcher

PubMed 28879061

DOI 10.7717/peerj.3702

Crossref 10.7717/peerj.3702

pii: 3702
pmc: PMC5582611

Publications 9.5.0