Generation of tumor spheroids in microwells to study NK cell cytotoxicity, infiltration and phenotype.

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Carannante V, Sandström N, Olofsson K, Van Ooijen H, Hell B, Wiklund M, Önfelt B

Methods Cell Biol 178 (-) 195-208 [2023-02-16; online 2023-02-16]

The development of new immunotherapeutic drugs and combinatorial strategies requires the implementation of novel methods to test their efficacy in vitro. Here, we present a series of miniaturized in vitro assays to assess immune cell cytotoxic activity, infiltration, and phenotype in renal carcinoma spheroids with the use of a recently developed multichambered microwell chip. We provide protocols for tumor spheroid formation, NK cell culture, fluorescence labelling and imaging of live or fixed cells directly in the chip together with data analysis.

PubMed 37516526

DOI 10.1016/bs.mcb.2023.01.001

Crossref 10.1016/bs.mcb.2023.01.001

pii: S0091-679X(23)00001-8