Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source.

Munke A, Andreasson J, Aquila A, Awel S, Ayyer K, Barty A, Bean RJ, Berntsen P, Bielecki J, Boutet S, Bucher M, Chapman HN, Daurer BJ, DeMirci H, Elser V, Fromme P, Hajdu J, Hantke MF, Higashiura A, Hogue BG, Hosseinizadeh A, Kim Y, Kirian RA, Reddy HK, Lan TY, Larsson DS, Liu H, Loh ND, Maia FR, Mancuso AP, Mühlig K, Nakagawa A, Nam D, Nelson G, Nettelblad C, Okamoto K, Ourmazd A, Rose M, van der Schot G, Schwander P, Seibert MM, Sellberg JA, Sierra RG, Song C, Svenda M, Timneanu N, Vartanyants IA, Westphal D, Wiedorn MO, Williams GJ, Xavier PL, Yoon CH, Zook J

Sci Data 3 (-) 160064 [2016-08-01; online 2016-08-01]

Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.

PubMed 27478984

DOI 10.1038/sdata.2016.64

Crossref 10.1038/sdata.2016.64

pmc: PMC4968191
pii: sdata201664
figshare: 10.6084/M9.FIGSHARE.C.2342581