Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

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Konold PE, Monrroy L, Bellisario A, Filipe D, Adams P, Alvarez R, Bean R, Bielecki J, Bódizs S, Ducrocq G, Grubmueller H, Kirian RA, Kloos M, Koliyadu JCP, Koua FHM, Larkiala T, Letrun R, Lindsten F, Maihöfer M, Martin AV, Mészáros P, Mutisya J, Nimmrich A, Okamoto K, Round A, Sato T, Valerio J, Westphal D, Wollter A, Yenupuri TV, You T, Maia F, Westenhoff S

Nat. Methods 21 (9) 1608-1611 [2024-09-00; online 2024-07-05]

Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.

PubMed 38969722

DOI 10.1038/s41592-024-02344-0

Crossref 10.1038/s41592-024-02344-0

pmc: PMC11399097
pii: 10.1038/s41592-024-02344-0