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J. Proteome Res. 22 (4) 1359-1366 [2023-04-07; online 2023-03-29]
A frequent goal, or subgoal, when processing data from a quantitative shotgun proteomics experiment is a list of proteins that are differentially abundant under the examined experimental conditions. Unfortunately, obtaining such a list is a challenging process, as the mass spectrometer analyzes the proteolytic peptides of a protein rather than the proteins themselves. We have previously designed a Bayesian hierarchical probabilistic model, Triqler, for combining peptide identification and quantification errors into probabilities of proteins being differentially abundant. However, the model was developed for data from data-dependent acquisition. Here, we show that Triqler is also compatible with data-independent acquisition data after applying minor alterations for the missing value distribution. Furthermore, we find that it has better performance than a set of compared state-of-the-art protein summarization tools when evaluated on data-independent acquisition data.
PubMed 36988210
DOI 10.1021/acs.jproteome.2c00607
Crossref 10.1021/acs.jproteome.2c00607