Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle Amplification.

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Zhao Y, Lin C, Wu P, Chen X, Zhao Y, Li Y, Chen L, Nilsson M, Ke R

ACS Sens 5 (10) 3031-3036 [2020-10-23; online 2020-09-24]

Conventional flow cytometry has been widely used for high-throughput single-cell gene expression analysis using specific antibody staining. However, this is limited by the availability of high-quality antibodies. We developed a novel flow cytometry RNA detection technique termed RCA-Flow for single-cell RNA expression analysis. We showed that it is able to analyze not only mRNAs but also microRNAs and circular RNAs that are otherwise difficult to analyze by other flow cytometry techniques. The versatility for high-throughput analysis of different types of RNA molecules makes our method possess great potential for both biomedical and clinical applications.

PubMed 32935538

DOI 10.1021/acssensors.0c01569

Crossref 10.1021/acssensors.0c01569