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Hansen JN
Methods in molecular biology (Clifton, N.J.) 2713 (-) 519-541 [2023-08-28; online 2023-08-28]
Cell morphology and motility drive the cellular capabilities to interact with the environment. For example, microglia, the longest known tissue-resident macrophages, show a highly branched process tree with which they continuously scan their environment. Computational image analysis allows to quantify morphology and/or motility from images of tissue-resident macrophages. Here, I describe a step-by-step protocol for analyzing the morphology (and motility) of macrophages with our recently described, freely available software MotiQ, which provides a broad band of parameters and thereby serves as a versatile tool for studies of morphology and motility.
PubMed 37639145
DOI 10.1007/978-1-0716-3437-0_34
Crossref 10.1007/978-1-0716-3437-0_34