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Fan Y, Andrusivová Ž, Wu Y, Chai C, Larsson L, He M, Luo L, Lundeberg J, Wang B
Nat. Methods 20 (8) 1179-1182 [2023-08-00; online 2023-06-22]
Capture array-based spatial transcriptomics methods have been widely used to resolve gene expression in tissues; however, their spatial resolution is limited by the density of the array. Here we present expansion spatial transcriptomics to overcome this limitation by clearing and expanding tissue prior to capturing the entire polyadenylated transcriptome with an enhanced protocol. This approach enables us to achieve higher spatial resolution while retaining high library quality, which we demonstrate using mouse brain samples.
PubMed 37349575
DOI 10.1038/s41592-023-01911-1
Crossref 10.1038/s41592-023-01911-1
mid: NIHMS1982782
pmc: PMC11078125
pii: 10.1038/s41592-023-01911-1