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Schuster J, Fatima A, Papadopoulos N, de Guidi C, Sobol M, Dahl N
Stem Cell Res 80 (-) 103521 [2024-10-00; online 2024-07-31]
The transcription factor ZEB2 is essential for early embryonic development. Using CRISPR/Cas9, we generated a ZEB2 deficient human iPSC cell line (KICRi002A-4), carrying a homozygous 790 bp deletion in ZEB2 that involves part of exon 5, intron 5 and part of exon 6. The deletion leads to markedly reduced levels of a truncated ZEB2 transcript. No ZEB2 protein was detected by immunopreciptation. The iPSC line expressed pluripotency markers and showed a capacity to differentiate into the three germ layers in vitro. Assessment of genomic integrity revealed a normal karyotype without detectable OFF-target editing. The iPSC line KICRi002A-4 thus offers a valuable resource to study the role of ZEB2 for the commitment and differentiation of various human cell lineages.
PubMed 39121652
DOI 10.1016/j.scr.2024.103521
Crossref 10.1016/j.scr.2024.103521
pii: S1873-5061(24)00219-8