{"entity": "researcher", "timestamp": "2026-08-28T00:26:13.327Z", "family": "Stratigi", "given": "Kalliopi", "initials": "K", "orcid": "0000-0001-7476-6410", "affiliations": ["Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, GR70013, Heraklion, Crete, Greece."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/fd96aecde8a845d9a9cca71822b4265a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/fd96aecde8a845d9a9cca71822b4265a"}}, "publications": [{"entity": "publication", "iuid": "48d7230da2b64ce9b139347443337557", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/48d7230da2b64ce9b139347443337557.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/48d7230da2b64ce9b139347443337557"}}, "title": "XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes.", "authors": [{"family": "Chatzinikolaou", "given": "Georgia", "initials": "G", "orcid": "0000-0002-0868-0683", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7f6e7f7f9fe64cbe973761f86f7da642.json"}}, {"family": "Stratigi", "given": "Kalliopi", "initials": "K", "orcid": "0000-0001-7476-6410", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fd96aecde8a845d9a9cca71822b4265a.json"}}, {"family": "Siametis", "given": "Athanasios", "initials": "A", "orcid": "0000-0003-0668-7563", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1b894b3ff9c14a93990bb0f852d1c9c0.json"}}, {"family": "Goulielmaki", "given": "Evi", "initials": "E", "orcid": "0000-0003-1651-3137", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bb232191da7f4d8aa506326d0bceb462.json"}}, {"family": "Akalestou-Clocher", "given": "Alexia", "initials": "A", "orcid": "0000-0002-1267-6054", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/74e4070a6ef945948f1f190216be437a.json"}}, {"family": "Tsamardinos", "given": "Ioannis", "initials": "I", "orcid": "0000-0002-2492-959X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d760c8cda394426fbcf21bd74f711120.json"}}, {"family": "Topalis", "given": "Pantelis", "initials": "P", "orcid": "0000-0002-1635-4810", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0749268a2e9d4a88b89fac3ace21700e.json"}}, {"family": "Austin", "given": "Caroline", "initials": "C"}, {"family": "Bouwman", "given": "Britta A M", "initials": "BAM", "orcid": "0000-0002-9827-9497", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/26e2947dca1e4467aef5342387c5ca7d.json"}}, {"family": "Crosetto", "given": "Nicola", "initials": "N"}, {"family": "Altm\u00fcller", "given": "Janine", "initials": "J"}, {"family": "Garinis", "given": "George A", "initials": "GA", "orcid": "0000-0002-3200-5004", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9628812e29b548c1893ffb87e52e91e7.json"}}], "type": "journal article", "published": "2023-11-10", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "9", "issue": "45", "pages": "eadi2095", "issn-l": "2375-2548"}, "abstract": "Co-transcriptional RNA-DNA hybrids can not only cause DNA damage threatening genome integrity but also regulate gene activity in a mechanism that remains unclear. Here, we show that the nucleotide excision repair factor XPF interacts with the insulator binding protein CTCF and the cohesin subunits SMC1A and SMC3, leading to R-loop-dependent DNA looping upon transcription activation. To facilitate R-loop processing, XPF interacts and recruits with TOP2B on active gene promoters, leading to double-strand break accumulation and the activation of a DNA damage response. Abrogation of TOP2B leads to the diminished recruitment of XPF, CTCF, and the cohesin subunits to promoters of actively transcribed genes and R-loops and the concurrent impairment of CTCF-mediated DNA looping. Together, our findings disclose an essential role for XPF with TOP2B and the CTCF/cohesin complex in R-loop processing for transcription activation with important ramifications for DNA repair-deficient syndromes associated with transcription-associated DNA damage.", "doi": "10.1126/sciadv.adi2095", "pmid": "37939182", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10631727"}], "notes": [], "created": "2026-08-21T12:22:29.404Z", "modified": "2026-08-21T12:22:29.704Z"}]}