{"entity": "researcher", "timestamp": "2026-08-23T10:18:43.972Z", "family": "Soukup", "given": "Ondrej", "initials": "O", "orcid": "0000-0001-6376-8701", "affiliations": ["Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove 500 05, Czech Republic."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/fce4207229ad45349ae434af79d277f4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/fce4207229ad45349ae434af79d277f4"}}, "publications": [{"entity": "publication", "iuid": "23a842334d594221bedbe0cf5ff9cd28", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/23a842334d594221bedbe0cf5ff9cd28.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/23a842334d594221bedbe0cf5ff9cd28"}}, "title": "Discovery of a 6-Aminobenzo[b]thiophene 1,1-Dioxide Derivative (K2071) with a Signal Transducer and Activator of Transcription 3 Inhibitory, Antimitotic, and Senotherapeutic Activities.", "authors": [{"family": "Oleksak", "given": "Patrik", "initials": "P", "orcid": "0000-0002-6850-7671", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/346b2e1937fd4ff0a4a0fa895ae635aa.json"}}, {"family": "Rysanek", "given": "David", "initials": "D", "orcid": "0000-0002-5131-0055", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8dc6659a04f9480799fcad9c1825aff2.json"}}, {"family": "Vancurova", "given": "Marketa", "initials": "M"}, {"family": "Vasicova", "given": "Pavla", "initials": "P"}, {"family": "Urbancokova", "given": "Alexandra", "initials": "A"}, {"family": "Novak", "given": "Josef", "initials": "J"}, {"family": "Maurencova", "given": "Dominika", "initials": "D"}, {"family": "Kashmel", "given": "Pavel", "initials": "P"}, {"family": "Houserova", "given": "Jana", "initials": "J"}, {"family": "Mikyskova", "given": "Romana", "initials": "R"}, {"family": "Novotny", "given": "Ondrej", "initials": "O"}, {"family": "Reinis", "given": "Milan", "initials": "M", "orcid": "0000-0001-7083-2898", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/37bb3c1ba48b45ce9dc905739c01d76a.json"}}, {"family": "Juda", "given": "Pavel", "initials": "P", "orcid": "0000-0002-6421-3852", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9b178b9b5af14b7eb8efa85195b11c94.json"}}, {"family": "Hons", "given": "Miroslav", "initials": "M"}, {"family": "Kroupova", "given": "Jirina", "initials": "J", "orcid": "0000-0002-7946-6975", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b214bd2e8ebb4227a10dfc292184a1ce.json"}}, {"family": "Sedlak", "given": "David", "initials": "D", "orcid": "0000-0003-0074-335X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f82128dbfc234876af9d3435a024b8b2.json"}}, {"family": "Sulimenko", "given": "Tetyana", "initials": "T"}, {"family": "Draber", "given": "Pavel", "initials": "P"}, {"family": "Chlubnova", "given": "Marketa", "initials": "M"}, {"family": "Nepovimova", "given": "Eugenie", "initials": "E"}, {"family": "Kuca", "given": "Kamil", "initials": "K", "orcid": "0000-0001-9664-1109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ae2c926b546c44cc87de82bc159801c4.json"}}, {"family": "Lisa", "given": "Miroslav", "initials": "M", "orcid": "0000-0001-9227-5375", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/384738aa92884e36b3e9703588a75b4c.json"}}, {"family": "Andrys", "given": "Rudolf", "initials": "R"}, {"family": "Kobrlova", "given": "Tereza", "initials": "T"}, {"family": "Soukup", "given": "Ondrej", "initials": "O", "orcid": "0000-0001-6376-8701", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fce4207229ad45349ae434af79d277f4.json"}}, {"family": "Janousek", "given": "Jiri", "initials": "J"}, {"family": "Prchal", "given": "Lukas", "initials": "L"}, {"family": "Bartek", "given": "Jiri", "initials": "J"}, {"family": "Musilek", "given": "Kamil", "initials": "K"}, {"family": "Hodny", "given": "Zdenek", "initials": "Z"}], "type": "journal article", "published": "2024-09-13", "journal": {"title": "ACS pharmacology & translational science", "issn": "2575-9108", "volume": "7", "issue": "9", "pages": "2755-2783", "issn-l": "2575-9108"}, "abstract": "6-Nitrobenzo[b]thiophene 1,1-dioxide (Stattic) is a potent signal transducer and activator of the transcription 3 (STAT3) inhibitor developed originally for anticancer therapy. However, Stattic harbors several STAT3 inhibition-independent biological effects. To improve the properties of Stattic, we prepared a series of analogues derived from 6-aminobenzo[b]thiophene 1,1-dioxide, a compound directly obtained from the reduction of Stattic, that includes a methoxybenzylamino derivative (K2071) with optimized physicochemical characteristics, including the ability to cross the blood-brain barrier. Besides inhibiting the interleukin-6-stimulated activity of STAT3 mediated by tyrosine 705 phosphorylation, K2071 also showed cytotoxicity against a set of human glioblastoma-derived cell lines. In contrast to the core compound, a part of K2071 cytotoxicity reflected a STAT3 inhibition-independent block of mitotic progression in the prophase, affecting mitotic spindle formation, indicating that K2071 also acts as a mitotic poison. Compared to Stattic, K2071 was significantly less thiol-reactive. In addition, K2071 affected cell migration, suppressed cell proliferation in tumor spheroids, exerted cytotoxicity for glioblastoma temozolomide-induced senescent cells, and inhibited the secretion of the proinflammatory cytokine monocyte chemoattractant protein 1 (MCP-1) in senescent cells. Importantly, K2071 was well tolerated in mice, lacking manifestations of acute toxicity. The structure-activity relationship analysis of the K2071 molecule revealed the necessity of the para-substituted methoxyphenyl motif for antimitotic but not overall cytotoxic activity of its derivatives. Altogether, these results indicate that compound K2071 is a novel Stattic-derived STAT3 inhibitor and a mitotic poison with anticancer and senotherapeutic properties that is effective on glioblastoma cells and may be further developed as an agent for glioblastoma therapy.", "doi": "10.1021/acsptsci.4c00190", "pmid": "39296273", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11406704"}], "notes": [], "created": "2026-08-20T08:12:58.594Z", "modified": "2026-08-20T08:13:22.129Z"}, {"entity": "publication", "iuid": "90b15ea420ad46cfa2b10e96ae9d3cb5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/90b15ea420ad46cfa2b10e96ae9d3cb5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/90b15ea420ad46cfa2b10e96ae9d3cb5"}}, "title": "The Effect of Chemical Structure of OEG Ligand Shells with Quaternary Ammonium Moiety on the Colloidal Stabilization, Cellular Uptake and Photothermal Stability of Gold Nanorods.", "authors": [{"family": "Salajkova", "given": "Sarka", "initials": "S"}, {"family": "Havel", "given": "Filip", "initials": "F"}, {"family": "Sramek", "given": "Michal", "initials": "M"}, {"family": "Novotny", "given": "Filip", "initials": "F", "orcid": "0000-0003-0471-1865", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4bd871b56dae4fdb82030bbb92f46021.json"}}, {"family": "Malinak", "given": "David", "initials": "D", "orcid": "0000-0002-0665-0667", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/965853a26ded40cb9a83900a46b7e8b9.json"}}, {"family": "Dolezal", "given": "Rafael", "initials": "R", "orcid": "0000-0001-9495-3934", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9a386fd3b3e947178452837bce85d057.json"}}, {"family": "Prchal", "given": "Lukas", "initials": "L"}, {"family": "Benkova", "given": "Marketa", "initials": "M", "orcid": "0000-0002-9196-9073", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3a921bdc67ad47fd877e9e5d610018bb.json"}}, {"family": "Soukup", "given": "Ondrej", "initials": "O", "orcid": "0000-0001-6376-8701", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fce4207229ad45349ae434af79d277f4.json"}}, {"family": "Musilek", "given": "Kamil", "initials": "K", "orcid": "0000-0002-7504-4062", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d824d42026a488c8525588cd069144c.json"}}, {"family": "Kuca", "given": "Kamil", "initials": "K", "orcid": "0000-0001-9664-1109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ae2c926b546c44cc87de82bc159801c4.json"}}, {"family": "Bartek", "given": "Jiri", "initials": "J"}, {"family": "Proska", "given": "Jan", "initials": "J", "orcid": "0000-0002-2315-493X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/611aa9a2ad3147019f7f8c43faa7f52a.json"}}, {"family": "Zarska", "given": "Monika", "initials": "M", "orcid": "0000-0001-7023-760X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d55e9c4de3a14cfe9310b7764d320ed6.json"}}, {"family": "Hodny", "given": "Zdenek", "initials": "Z"}], "type": "journal article", "published": "2021-05-18", "journal": {"title": "Int J Nanomedicine", "issn": "1178-2013", "volume": "16", "pages": "3407-3427", "issn-l": "1176-9114"}, "abstract": "Plasmonic photothermal cancer therapy by gold nanorods (GNRs) emerges as a promising tool for cancer treatment. The goal of this study was to design cationic oligoethylene glycol (OEG) compounds varying in hydrophobicity and molecular electrostatic potential as ligand shells of GNRs. Three series of ligands with different length of OEG chain (ethylene glycol units = 3, 4, 5) and variants of quaternary ammonium salts (QAS) as terminal functional group were synthesized and compared to a prototypical quaternary ammonium ligand with alkyl chain - (16-mercaptohexadecyl)trimethylammonium bromide (MTAB).\n\nStep-by-step research approach starting with the preparation of compounds characterized by NMR and HRMS spectra, GNRs ligand exchange evaluation through characterization of cytotoxicity and GNRs cellular uptake was used. A method quantifying the reshaping of GNRs was applied to determine the effect of ligand structure on the heat transport from GNRs under fs-laser irradiation.\n\nFourteen out of 18 synthesized OEG compounds successfully stabilized GNRs in the water. The colloidal stability of prepared GNRs in the cell culture medium decreased with the number of OEG units. In contrast, the cellular uptake of OEG+GNRs by HeLa cells increased with the length of OEG chain while the structure of the QAS group showed a minor role. Compared to MTAB, more hydrophilic OEG compounds exhibited nearly two order of magnitude lower cytotoxicity in free state and provided efficient cellular uptake of GNRs close to the level of MTAB. Regarding photothermal properties, OEG compounds evoked the photothermal reshaping of GNRs at lower peak fluence (14.8 mJ/cm2) of femtosecond laser irradiation than the alkanethiol MTAB.\n\nOEG+GNRs appear to be optimal for clinical applications with systemic administration of NPs not-requiring irradiation at high laser intensity such as drug delivery and photothermal therapy inducing apoptosis.", "doi": "10.2147/IJN.S304953", "pmid": "34040371", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8140906"}, {"db": "pii", "key": "304953"}], "notes": [], "created": "2026-08-20T13:02:17.896Z", "modified": "2026-08-21T09:34:49.682Z"}]}