{"entity": "researcher", "timestamp": "2026-05-12T21:35:30.543Z", "family": "Pejler", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-6779-391X", "affiliations": ["Department of Medical Biochemistry and MicrobiologyUppsala University Uppsala Sweden", "Department of Anatomy, Physiology and BiochemistrySwedish University of Agricultural Sciences Uppsala Sweden"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/f84b69f010b746af82960c61ed561ba4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/f84b69f010b746af82960c61ed561ba4"}}, "publications": [{"entity": "publication", "iuid": "b806dca64373459bbae4857ebffd0557", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b806dca64373459bbae4857ebffd0557.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b806dca64373459bbae4857ebffd0557"}}, "title": "Dynamin inhibition causes context-dependent cell death of leukemia and lymphoma cells.", "authors": [{"family": "von Beek", "given": "Christopher", "initials": "C", "orcid": "0000-0001-6310-7583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fba39ea4c0f74e65a1a61e1ad1fb7b73.json"}}, {"family": "Alriksson", "given": "Linn\u00e9a", "initials": "L"}, {"family": "Palle", "given": "Josefine", "initials": "J"}, {"family": "Gustafson", "given": "Ann-Marie", "initials": "AM"}, {"family": "Grujic", "given": "Mirjana", "initials": "M"}, {"family": "Melo", "given": "Fabio Rabelo", "initials": "FR"}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Pejler", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-6779-391X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f84b69f010b746af82960c61ed561ba4.json"}}], "type": "journal article", "published": "2021-09-07", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "16", "issue": "9", "pages": "e0256708", "issn-l": "1932-6203"}, "abstract": "Current chemotherapy for treatment of pediatric acute leukemia, although generally successful, is still a matter of concern due to treatment resistance, relapses and life-long side effects for a subset of patients. Inhibition of dynamin, a GTPase involved in clathrin-mediated endocytosis and regulation of the cell cycle, has been proposed as a potential anti-cancer regimen, but the effects of dynamin inhibition on leukemia cells has not been extensively addressed. Here we adopted single cell and whole-population analysis by flow cytometry and live imaging, to assess the effect of dynamin inhibition (Dynasore, Dyngo-4a, MitMAB) on pediatric acute leukemia cell lines (CCRF-CEM and THP-1), human bone marrow biopsies from patients diagnosed with acute lymphoblastic leukemia (ALL), as well as in a model of lymphoma (EL4)-induced tumor growth in mice. All inhibitors suppressed proliferation and induced pronounced caspase-dependent apoptotic cell death in CCRF-CEM and THP-1 cell lines. However, the inhibitors showed no effect on bone marrow biopsies, and did not prevent EL4-induced tumor formation in mice. We conclude that dynamin inhibition affects highly proliferating human leukemia cells. These findings form a basis for evaluation of the potential, and constraints, of employing dynamin inhibition in treatment strategies against leukemia and other malignancies.", "doi": "10.1371/journal.pone.0256708", "pmid": "34492077", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pii", "key": "PONE-D-21-09071"}, {"db": "pmc", "key": "PMC8423305"}], "notes": [], "created": "2021-12-05T09:46:50.010Z", "modified": "2022-11-04T11:32:12.556Z"}, {"entity": "publication", "iuid": "f45288afd4b34b7996f46f75952f30e5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f45288afd4b34b7996f46f75952f30e5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f45288afd4b34b7996f46f75952f30e5"}}, "title": "Streptococcal sagA activates a proinflammatory response in mast cells by a sublytic mechanism.", "authors": [{"family": "von Beek", "given": "Christopher", "initials": "C", "orcid": "0000-0001-6310-7583", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fba39ea4c0f74e65a1a61e1ad1fb7b73.json"}}, {"family": "Waern", "given": "Ida", "initials": "I"}, {"family": "Eriksson", "given": "Jens", "initials": "J"}, {"family": "Melo", "given": "Fabio Rabelo", "initials": "FR"}, {"family": "Robinson", "given": "Carl", "initials": "C"}, {"family": "Waller", "given": "Andrew S", "initials": "AS"}, {"family": "Sellin", "given": "Mikael E", "initials": "ME", "orcid": "0000-0002-8355-0803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/559c18ec87d64318a0afc6267ea879e2.json"}}, {"family": "Guss", "given": "Bengt", "initials": "B"}, {"family": "Pejler", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-6779-391X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f84b69f010b746af82960c61ed561ba4.json"}}], "type": "journal article", "published": "2019-09-00", "journal": {"title": "Cell. Microbiol.", "issn": "1462-5822", "issn-l": "1462-5814", "volume": "21", "issue": "9", "pages": "e13064"}, "abstract": "Mast cells are implicated in the innate proinflammatory immune defence against bacterial insult, but the mechanisms through which mast cells respond to bacterial encounter are poorly defined. Here, we addressed this issue and show that mast cells respond vividly to wild type Streptococcus equi by up-regulating a panel of proinflammatory genes and by secreting proinflammatory cytokines. However, this response was completely abrogated when the bacteria lacked expression of sagA, whereas the lack of a range of other potential virulence genes (seeH, seeI, seeL, seeM, hasA, seM, aroB, pyrC, and recA) had no effect on the amplitude of the mast cell responses. The sagA gene encodes streptolysin S, a lytic toxin, and we next showed that the wild type strain but not a sagA-deficient mutant induced lysis of mast cells. To investigate whether host cell membrane perturbation per se could play a role in the activation of the proinflammatory response, we evaluated the effects of detergent- and pneumolysin-dependent lysis on mast cells. Indeed, exposure of mast cells to sublytic concentrations of all these agents resulted in cytokine responses of similar amplitudes as those caused by wild type streptococci. This suggests that sublytic membrane perturbation is sufficient to trigger full-blown proinflammatory signalling in mast cells. Subsequent analysis showed that the p38 and Erk1/2 signalling pathways had central roles in the proinflammatory response of mast cells challenged by either sagA-expressing streptococci or detergent. Altogether, these findings suggest that sagA-dependent mast cell membrane perturbation is a mechanism capable of activating the innate immune response upon bacterial challenge.", "doi": "10.1111/cmi.13064", "pmid": "31155820", "labels": {"Mikael Sellin": null, "SciLifeLab Fellow": null}, "xrefs": [{"db": "pmc", "key": "PMC6771685"}], "notes": [], "created": "2020-10-05T15:10:07.492Z", "modified": "2022-11-04T11:32:16.004Z"}]}