{"entity": "researcher", "timestamp": "2026-08-26T22:48:23.513Z", "family": "Damiati", "given": "Samar", "initials": "S", "orcid": "0000-0002-6747-0408", "affiliations": ["Department of BiochemistryFaculty of ScienceKing Abdulaziz University Jeddah 21589 Saudi Arabia.", "Division of NanobiotechnologyDepartment of Protein Science, Science for Life LaboratoryKTH Royal Institute of Technology 171 21 Stockholm Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4bcaf1725804cd9a1166885c0596486.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4bcaf1725804cd9a1166885c0596486"}}, "publications": [{"entity": "publication", "iuid": "3e7d668b57fb4d8da93c626ddde85ea7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3e7d668b57fb4d8da93c626ddde85ea7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3e7d668b57fb4d8da93c626ddde85ea7"}}, "title": "Enhancing the Cell-Free Expression of Native Membrane Proteins by In Silico Optimization of the Coding Sequence-An Experimental Study of the Human Voltage-Dependent Anion Channel.", "authors": [{"family": "Zayni", "given": "Sonja", "initials": "S"}, {"family": "Damiati", "given": "Samar", "initials": "S", "orcid": "0000-0002-6747-0408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4bcaf1725804cd9a1166885c0596486.json"}}, {"family": "Moreno-Flores", "given": "Susana", "initials": "S"}, {"family": "Amman", "given": "Fabian", "initials": "F", "orcid": "0000-0002-8646-859X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/afe4e1dd33254da081004eca07b526d7.json"}}, {"family": "Hofacker", "given": "Ivo", "initials": "I", "orcid": "0000-0001-7132-0800", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c73d110a12924a9098a8191c07e0dbac.json"}}, {"family": "Jin", "given": "David", "initials": "D"}, {"family": "Ehmoser", "given": "Eva-Kathrin", "initials": "EK", "orcid": "0000-0001-9201-268X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d9873347c6144a058d63c2a66fd4a22d.json"}}], "type": "journal article", "published": "2021-09-28", "journal": {"title": "Membranes (Basel)", "issn": "2077-0375", "volume": "11", "issue": "10", "issn-l": "2077-0375"}, "abstract": "Membrane proteins are involved in many aspects of cellular biology; for example, they regulate how cells interact with their environment, so such proteins are important drug targets. The rapid advancement in the field of immune effector cell therapy has been expanding the horizons of synthetic membrane receptors in the areas of cell-based immunotherapy and cellular medicine. However, the investigation of membrane proteins, which are key constituents of cells, is hampered by the difficulty and complexity of their in vitro synthesis, which is of unpredictable yield. Cell-free synthesis is herein employed to unravel the impact of the expression construct on gene transcription and translation, without the complex regulatory mechanisms of cellular systems. Through the systematic design of plasmids in the immediacy of the start of the target gene, it was possible to identify translation initiation and the conformation of mRNA as the main factors governing the cell-free expression efficiency of the human voltage-dependent anion channel (VDAC), which is a relevant membrane protein in drug-based therapy. A simple translation initiation model was developed to quantitatively assess the expression potential for the designed constructs. A scoring function that quantifies the feasibility of the formation of the translation initiation complex through the ribosome-mRNA hybridization energy and the accessibility of the mRNA segment binding to the ribosome is proposed. The scoring function enables one to optimize plasmid sequences and semi-quantitatively predict protein expression efficiencies. This scoring function is publicly available as webservice XenoExpressO at University of Vienna, Austria.", "doi": "10.3390/membranes11100741", "pmid": "34677509", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8540592"}, {"db": "pii", "key": "membranes11100741"}], "notes": [], "created": "2026-08-21T13:04:11.385Z", "modified": "2026-08-21T13:04:11.527Z"}, {"entity": "publication", "iuid": "2581456815bd4e7a826235cdc0cb266f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2581456815bd4e7a826235cdc0cb266f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2581456815bd4e7a826235cdc0cb266f"}}, "title": "Flex Printed Circuit Board Implemented Graphene-Based DNA Sensor for Detection of SARS-CoV-2.", "authors": [{"family": "Damiati", "given": "Samar", "initials": "S", "orcid": "0000-0002-6747-0408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4bcaf1725804cd9a1166885c0596486.json"}}, {"family": "Sopstad", "given": "Sindre", "initials": "S"}, {"family": "Peacock", "given": "Martin", "initials": "M"}, {"family": "Akhtar", "given": "Ahmad S", "initials": "AS", "orcid": "0000-0002-4560-4735", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f3dc0fe88bd41f2a435b2c0a7d46238.json"}}, {"family": "Pinto", "given": "Ines", "initials": "I", "orcid": "0000-0002-9714-4742", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/47785e08c3f84b7ebdcbf4b8b979ef21.json"}}, {"family": "Soares", "given": "Ruben R G", "initials": "RRG"}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2021-06-15", "journal": {"title": "IEEE Sens J", "issn": "1530-437X", "volume": "21", "issue": "12", "pages": "13060-13067", "issn-l": null}, "abstract": "Since the COVID-19 outbreak was declared a pandemic by the World Health Organization (WHO) in March 2020, ongoing efforts have been made to develop sensitive diagnostic platforms. Detection of viral RNA provides the highest sensitivity and specificity for detection of early and asymptomatic infections. Thus, this work aimed at developing a label-free genosensor composed of graphene as a working electrode that could be embedded into a flex printed circuit board (FPCB) for the rapid, sensitive, amplification-free and label-free detection of SARS-CoV-2. To facilitate liquid handling and ease of use, the developed biosensor was embedded with a user-friendly reservoir chamber. As a proof-of-concept, detection of a synthetic DNA strand matching the sequence of ORF1ab was performed as a two-step strategy involving the immobilization of a biotinylated complementary sequence on a streptavidin-modified surface, followed by hybridization with the target sequence recorded by the differential pulse voltammetric (DPV) technique in the presence of a ferro/ferricyanide redox couple. The effective design of the sensing platform improved its selectivity and sensitivity and allowed DNA quantification ranging from 100 fg/mL to [Formula: see text]/mL. Combining the electrochemical technique with FPCB enabled rapid detection of the target sequence using a small volume of the sample (5-[Formula: see text]). We achieved a limit-of-detection of 100 fg/mL, whereas the predicted value was ~33 fg/mL, equivalent to approximately [Formula: see text] copies/mL and comparable to sensitivities provided by isothermal nucleic acid amplification tests. We believe that the developed approach proves the ability of an FPCB-implemented DNA sensor to act as a potentially simpler and more affordable diagnostic assay for viral infections in Point-Of-Care (POC) applications.", "doi": "10.1109/JSEN.2021.3068922", "pmid": "35582203", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8864937"}], "notes": [], "created": "2026-08-20T11:16:41.207Z", "modified": "2026-08-21T09:29:48.702Z"}, {"entity": "publication", "iuid": "c38a82d957e549248934d1b0fb936bdb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c38a82d957e549248934d1b0fb936bdb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c38a82d957e549248934d1b0fb936bdb"}}, "title": "Electrochemical Biosensors Based on S-Layer Proteins.", "authors": [{"family": "Damiati", "given": "Samar", "initials": "S", "orcid": "0000-0002-6747-0408", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4bcaf1725804cd9a1166885c0596486.json"}}, {"family": "Schuster", "given": "Bernhard", "initials": "B"}], "type": "journal article", "published": "2020-03-19", "journal": {"title": "Sensors (Basel)", "issn": "1424-8220", "volume": "20", "issue": "6", "issn-l": null}, "abstract": "Designing and development of electrochemical biosensors enable molecule sensing and quantification of biochemical compositions with multitudinous benefits such as monitoring, detection, and feedback for medical and biotechnological applications. Integrating bioinspired materials and electrochemical techniques promote specific, rapid, sensitive, and inexpensive biosensing platforms for (e.g., point-of-care testing). The selection of biomaterials to decorate a biosensor surface is a critical issue as it strongly affects selectivity and sensitivity. In this context, smart biomaterials with the intrinsic self-assemble capability like bacterial surface (S-) layer proteins are of paramount importance. Indeed, by forming a crystalline two-dimensional protein lattice on many sensors surfaces and interfaces, the S-layer lattice constitutes an immobilization matrix for small biomolecules and lipid membranes and a patterning structure with unsurpassed spatial distribution for sensing elements and bioreceptors. This review aims to highlight on exploiting S-layer proteins in biosensor technology for various applications ranging from detection of metal ions over small organic compounds to cells. Furthermore, enzymes immobilized on the S-layer proteins allow specific detection of several vital biomolecules. The special features of the S-layer protein lattice as part of the sensor architecture enhances surface functionalization and thus may feature an innovative class of electrochemical biosensors.", "doi": "10.3390/s20061721", "pmid": "32204503", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7147708"}, {"db": "pii", "key": "s20061721"}], "notes": [], "created": "2026-08-21T13:05:11.266Z", "modified": "2026-08-21T13:05:11.323Z"}]}