{"entity": "researcher", "timestamp": "2026-08-22T07:49:20.181Z", "family": "Pettersson", "given": "Torbj\u00f6rn", "initials": "T", "orcid": "0000-0002-5444-7276", "affiliations": ["Fibre and Polymer Technology, KTH Royal Institute of Technology, Stockholm, 10044, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/f324e73b0d1e491280bf0bb973610a69.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/f324e73b0d1e491280bf0bb973610a69"}}, "publications": [{"entity": "publication", "iuid": "91dc7838bf8b4cb4aa99039bae7b75d0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/91dc7838bf8b4cb4aa99039bae7b75d0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/91dc7838bf8b4cb4aa99039bae7b75d0"}}, "title": "Assessing the Layer-by-Layer Assembly of Cellulose Nanofibrils and Polyelectrolytes in Pancreatic Tumor Spheroid Formation.", "authors": [{"family": "Abbasi Aval", "given": "Negar", "initials": "N", "orcid": "0000-0002-6544-9955", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f972e8b710be428299108c3459fd56c7.json"}}, {"family": "Lahchaichi", "given": "Ekeram", "initials": "E", "orcid": "0009-0005-3637-2513", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c7b007cf91af4fcf89c6dce2a7146706.json"}}, {"family": "Tudoran", "given": "Oana", "initials": "O", "orcid": "0000-0001-5082-9341", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9cf584d23e4042d1a4519fd0eb47d560.json"}}, {"family": "Fayazbakhsh", "given": "Farzaneh", "initials": "F"}, {"family": "Heuchel", "given": "Rainer", "initials": "R"}, {"family": "L\u00f6hr", "given": "Matthias", "initials": "M", "orcid": "0000-0002-7647-198X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1b209074b1e548ddad51414dab2d0b19.json"}}, {"family": "Pettersson", "given": "Torbj\u00f6rn", "initials": "T", "orcid": "0000-0002-5444-7276", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f324e73b0d1e491280bf0bb973610a69.json"}}, {"family": "Russom", "given": "Aman", "initials": "A"}], "type": "journal article", "published": "2023-11-15", "journal": {"title": "Biomedicines", "issn": "2227-9059", "volume": "11", "issue": "11", "issn-l": null}, "abstract": "Three-dimensional (3D) tumor spheroids are regarded as promising models for utilization as preclinical assessments of chemo-sensitivity. However, the creation of these tumor spheroids presents challenges, given that not all tumor cell lines are able to form consistent and regular spheroids. In this context, we have developed a novel layer-by-layer coating of cellulose nanofibril-polyelectrolyte bilayers for the generation of spheroids. This technique builds bilayers of cellulose nanofibrils and polyelectrolytes and is used here to coat two distinct 96-well plate types: nontreated/non-sterilized and Nunclon Delta. In this work, we optimized the protocol aimed at generating and characterizing spheroids on difficult-to-grow pancreatic tumor cell lines. Here, diverse parameters were explored, encompassing the bilayer count (five and ten) and multiple cell-seeding concentrations (10, 100, 200, 500, and 1000 cells per well), using four pancreatic tumor cell lines-KPCT, PANC-1, MiaPaCa-2, and CFPAC-I. The evaluation includes the quantification (number of spheroids, size, and morphology) and proliferation of the produced spheroids, as well as an assessment of their viability. Notably, our findings reveal a significant influence from both the number of bilayers and the plate type used on the successful formation of spheroids. The novel and simple layer-by-layer-based coating method has the potential to offer the large-scale production of spheroids across a spectrum of tumor cell lines.", "doi": "10.3390/biomedicines11113061", "pmid": "38002061", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10669291"}, {"db": "pii", "key": "biomedicines11113061"}], "notes": [], "created": "2026-08-20T13:38:54.829Z", "modified": "2026-08-20T13:38:55.045Z"}, {"entity": "publication", "iuid": "771a8b3253e946d195792991e6e4c569", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/771a8b3253e946d195792991e6e4c569.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/771a8b3253e946d195792991e6e4c569"}}, "title": "Layer-by-Layer Cellulose Nanofibrils: A New Coating Strategy for Development and Characterization of Tumor Spheroids as a Model for In Vitro Anticancer Drug Screening.", "authors": [{"family": "Aljadi", "given": "Zenib", "initials": "Z", "orcid": "0000-0003-1558-1154", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f6bc865217e1420399c49ea05fb9c8e0.json"}}, {"family": "Aval", "given": "Negar Abbasi", "initials": "NA"}, {"family": "Kumar", "given": "Tharagan", "initials": "T"}, {"family": "Qin", "given": "Taoyu", "initials": "T"}, {"family": "Ramachandraiah", "given": "Harisha", "initials": "H"}, {"family": "Pettersson", "given": "Torbj\u00f6rn", "initials": "T", "orcid": "0000-0002-5444-7276", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f324e73b0d1e491280bf0bb973610a69.json"}}, {"family": "Russom", "given": "Aman", "initials": "A", "orcid": "0000-0002-0242-358X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8939625551d5409aac4593329e2a6578.json"}}], "type": "journal article", "published": "2022-10-00", "journal": {"title": "Macromol Biosci", "issn": "1616-5195", "volume": "22", "issue": "10", "pages": "e2200137", "issn-l": "1616-5187"}, "abstract": "Three-dimensional multicellular spheroids (MCSs) are complex structure of cellular aggregates and cell-to-matrix interaction that emulates the in-vivo microenvironment. This research field has grown to develop and improve spheroid generation techniques. Here, we present a new platform for spheroid generation using Layer-by-Layer (LbL) technology. Layer-by-Layer (LbL) containing cellulose nanofibrils (CNF) assemble on a standard 96 well plate. Various bi-layer numbers, multiple cell seeding concentration, and two tumor cell lines (HEK 293 T, HCT 116) are utilized to generate and characterize spheroids. The number and proliferation of generated spheroids, the viability, and the response to the anti-cancer drug are examined. The spheroids are formed and proliferated on the LbL-CNF coated wells with no significant difference in connection to the number of LbL-CNF bi-layers; however, the number of formed spheroids correlates positively with the cell seeding concentration (122 \u00b1 17) and (42 \u00b1 8) for HCT 116 and HEK 293T respectively at 700 cells ml-1 . The spheroids proliferate progressively up to (309, 663) \u00b5m of HCT 116 and HEK 293T respectively on 5 bi-layers coated wells with maintaining viability. The (HCT 116) spheroids react to the anti-cancer drug. We demonstrate a new (LbL-CNF) coating strategy for spheroids generation, with high performance and efficiency to test anti-cancer drugs.", "doi": "10.1002/mabi.202200137", "pmid": "35899862", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:34:20.561Z", "modified": "2026-08-20T06:34:20.716Z"}]}