{"entity": "researcher", "timestamp": "2026-08-20T21:34:22.465Z", "family": "Larsen", "given": "Dorthe H", "initials": "DH", "orcid": "0000-0002-8280-1252", "affiliations": ["Nucleolar Stress and Disease Group, Danish Cancer Institute, 2100, Copenhagen, Denmark. dhl@cancer.dk."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2189ec69bc54980943fe1c35084f4c8.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2189ec69bc54980943fe1c35084f4c8"}}, "publications": [{"entity": "publication", "iuid": "1dddb1bd0d524404b39c4ae4dfed4ab6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1dddb1bd0d524404b39c4ae4dfed4ab6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1dddb1bd0d524404b39c4ae4dfed4ab6"}}, "title": "Hyper-recombination in ribosomal DNA is driven by long-range resection-independent RAD51 accumulation.", "authors": [{"family": "G\u00e1l", "given": "Zita", "initials": "Z"}, {"family": "Boukoura", "given": "Stavroula", "initials": "S", "orcid": "0000-0002-7176-103X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebea00b102f84139835c63ec36a11326.json"}}, {"family": "Oxe", "given": "Kezia Catharina", "initials": "KC", "orcid": "0000-0002-4921-4750", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3413af2fa0744ea49ece72eb2e709f95.json"}}, {"family": "Badawi", "given": "Sara", "initials": "S"}, {"family": "Nieto", "given": "Blanca", "initials": "B", "orcid": "0000-0001-9923-8534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1f92e8eb6b9d47c0bb8556ccb6beccbe.json"}}, {"family": "Korsholm", "given": "Lea Milling", "initials": "LM", "orcid": "0009-0007-9632-8957", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/98f369e2c96b4b4c89daf22d87af8cfb.json"}}, {"family": "Geisler", "given": "Sille Blangstrup", "initials": "SB", "orcid": "0009-0005-7052-9000", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fef45bbd28a64ca3a2e8cff0de1a842a.json"}}, {"family": "Dulina", "given": "Ekaterina", "initials": "E"}, {"family": "Rasmussen", "given": "Anna Vestergaard", "initials": "AV"}, {"family": "Dahl", "given": "Christina", "initials": "C", "orcid": "0000-0002-6177-2640", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e2ce41bd2a346cf94e5f42150a42261.json"}}, {"family": "Lv", "given": "Wei", "initials": "W"}, {"family": "Xu", "given": "Huixin", "initials": "H"}, {"family": "Pan", "given": "Xiaoguang", "initials": "X"}, {"family": "Arampatzis", "given": "Stefanos", "initials": "S"}, {"family": "Stratou", "given": "Danai-Eleni", "initials": "DE", "orcid": "0009-0002-7380-5152", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2b9aebe03f3c4847aa876a207b43172c.json"}}, {"family": "Galanos", "given": "Panagiotis", "initials": "P", "orcid": "0000-0003-1403-4685", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d1071fd28cb42d6b328182cddfd341f.json"}}, {"family": "Lin", "given": "Lin", "initials": "L", "orcid": "0000-0002-7546-4948", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bcc1572910494eb191e316307d213606.json"}}, {"family": "Guldberg", "given": "Per", "initials": "P", "orcid": "0000-0002-4424-4803", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/321fa32fa14a4704aefb37161651bb48.json"}}, {"family": "Bartek", "given": "Jiri", "initials": "J", "orcid": "0000-0003-2013-7525", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/288939950c0c4c6393d35c84e5128b5f.json"}}, {"family": "Luo", "given": "Yonglun", "initials": "Y", "orcid": "0000-0002-0007-7759", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f0283cb6c284428f906b0d03fda62a1e.json"}}, {"family": "Larsen", "given": "Dorthe H", "initials": "DH", "orcid": "0000-0002-8280-1252", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2189ec69bc54980943fe1c35084f4c8.json"}}], "type": "journal article", "published": "2024-09-06", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "7797", "issn-l": "2041-1723"}, "abstract": "Ribosomal DNA (rDNA) encodes the ribosomal RNA genes and represents an intrinsically unstable genomic region. However, the underlying mechanisms and implications for genome integrity remain elusive. Here, we use Bloom syndrome (BS), a rare genetic disease characterized by DNA repair defects and hyper-unstable rDNA, as a model to investigate the mechanisms leading to rDNA instability. We find that in Bloom helicase (BLM) proficient cells, the homologous recombination (HR) pathway in rDNA resembles that in nuclear chromatin; it is initiated by resection, replication protein A (RPA) loading and BRCA2-dependent RAD51 filament formation. However, BLM deficiency compromises RPA-loading and BRCA1/2 recruitment to rDNA, but not RAD51 accumulation. RAD51 accumulates at rDNA despite depletion of long-range resection nucleases and rDNA damage results in micronuclei when BLM is absent. In summary, our findings indicate that rDNA is permissive to RAD51 accumulation in the absence of BLM, leading to micronucleation and potentially global genomic instability.", "doi": "10.1038/s41467-024-52189-6", "pmid": "39242676", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11379943"}, {"db": "pii", "key": "10.1038/s41467-024-52189-6"}], "notes": [], "created": "2026-08-20T08:53:38.418Z", "modified": "2026-08-20T08:53:38.808Z"}, {"entity": "publication", "iuid": "8789ecfa7a464831bec5b0ca5f6bef92", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8789ecfa7a464831bec5b0ca5f6bef92.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8789ecfa7a464831bec5b0ca5f6bef92"}}, "title": "RNA Polymerase II-mediated transcription is required for repair of ribosomal DNA breaks in nucleolar caps, guarding against genomic instability", "authors": [{"family": "Checa-Rodr\u00edguez", "given": "Cintia", "initials": "C", "orcid": "0000-0003-4838-7323", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6319a7b597854cb0983e2180fb8178fe.json"}}, {"family": "Su\u00e1rez-Quintero", "given": "Beatriz", "initials": "B", "orcid": "0000-0003-3804-6066", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed04c7ef1e824cf4ba79f4e592295d73.json"}}, {"family": "Contreras", "given": "Laura", "initials": "L", "orcid": "0000-0001-8385-4084", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d184caeb00e74b95ad3519f37df8828d.json"}}, {"family": "Korsholm", "given": "Lea", "initials": "L", "orcid": "0009-0007-9632-8957", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/98f369e2c96b4b4c89daf22d87af8cfb.json"}}, {"family": "Payne-Larsen", "given": "Dorthe Helena", "initials": "DH", "orcid": "0000-0002-8280-1252", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2189ec69bc54980943fe1c35084f4c8.json"}}, {"family": "de la Cruz", "given": "Jes\u00fas", "initials": "J", "orcid": "0000-0001-5870-659X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d45d9bdc77e4971972eceacc7a890dc.json"}}, {"family": "Bartek", "given": "Jiri", "initials": "J", "orcid": "0000-0003-2013-7525", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/288939950c0c4c6393d35c84e5128b5f.json"}}, {"family": "G\u00f3mez-Cabello", "given": "Daniel", "initials": "D", "orcid": "0000-0002-2807-8438", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eeea8f9881db4bf6a6473aba72a4f9c0.json"}}], "type": "posted-content", "published": "2023-10-22", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2023.10.20.563274", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:48:32.009Z", "modified": "2026-08-20T10:48:32.236Z"}, {"entity": "publication", "iuid": "8d2a0a15d056415f8b01dd5827db1154", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8d2a0a15d056415f8b01dd5827db1154.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8d2a0a15d056415f8b01dd5827db1154"}}, "title": "DNA damage-induced dynamic changes in abundance and cytosol-nuclear translocation of proteins involved in translational processes, metabolism, and autophagy.", "authors": [{"family": "Bennetzen", "given": "Martin V", "initials": "MV"}, {"family": "Kosar", "given": "Martin", "initials": "M"}, {"family": "Bunkenborg", "given": "Jakob", "initials": "J", "orcid": "0000-0002-2908-9323", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b7ce4efb2a53493382d4d83253a91008.json"}}, {"family": "Payne", "given": "Mark Ronald", "initials": "MR", "orcid": "0000-0001-5795-2481", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ae11eb7b61024efaa574d07249666957.json"}}, {"family": "Bartkova", "given": "Jirina", "initials": "J"}, {"family": "Lindstr\u00f6m", "given": "Mikael S", "initials": "MS"}, {"family": "Lukas", "given": "Jiri", "initials": "J"}, {"family": "Andersen", "given": "Jens S", "initials": "JS"}, {"family": "Bartek", "given": "Jiri", "initials": "J"}, {"family": "Larsen", "given": "Dorthe Helena", "initials": "DH", "orcid": "0000-0002-8280-1252", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f2189ec69bc54980943fe1c35084f4c8.json"}}], "type": "journal article", "published": "2018-09-22", "journal": {"title": "Cell Cycle", "issn": "1551-4005", "volume": "17", "issue": "17", "pages": "2146-2163", "issn-l": null}, "abstract": "Ionizing radiation (IR) causes DNA double-strand breaks (DSBs) and activates a versatile cellular response regulating DNA repair, cell-cycle progression, transcription, DNA replication and other processes. In recent years proteomics has emerged as a powerful tool deepening our understanding of this multifaceted response. In this study we use SILAC-based proteomics to specifically investigate dynamic changes in cytoplasmic protein abundance after ionizing radiation; we present in-depth bioinformatics analysis and show that levels of proteins involved in autophagy (cathepsins and other lysosomal proteins), proteasomal degradation (Ubiquitin-related proteins), energy metabolism (mitochondrial proteins) and particularly translation (ribosomal proteins and translation factors) are regulated after cellular exposure to ionizing radiation. Downregulation of no less than 68 ribosomal proteins shows rapid changes in the translation pattern after IR. Additionally, we provide evidence of compartmental cytosol-nuclear translocation of numerous DNA damage related proteins using protein correlation profiling. In conclusion, these results highlight unexpected cytoplasmic processes actively orchestrated after genotoxic insults and protein translocation from the cytoplasm to the nucleus as a fundamental regulatory mechanism employed to aid cell survival and preservation of genome integrity.", "doi": "10.1080/15384101.2018.1515552", "pmid": "30196736", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6226234"}], "notes": [], "created": "2026-08-20T09:36:48.743Z", "modified": "2026-08-20T09:36:48.895Z"}]}