{"entity": "researcher", "timestamp": "2026-10-13T18:52:08.793Z", "family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "affiliations": ["Science for Life Laboratory, Department of Environmental Science, Stockholm University, 11418 Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e"}}, "publications": [{"entity": "publication", "iuid": "ff22a5d26e8749979a34622dc085cf02", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ff22a5d26e8749979a34622dc085cf02.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ff22a5d26e8749979a34622dc085cf02"}}, "title": "Chemical Exposomics in Human Plasma by Lipid Removal and Large-Volume Injection Gas Chromatography-High-Resolution Mass Spectrometry.", "authors": [{"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/871a1de2d146412693d3cfea85e8e293.json"}}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Bergdahl", "given": "Ingvar A", "initials": "IA", "orcid": "0000-0003-1227-6859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9a988c282ed84691bb8432f2ae4082df.json"}}, {"family": "Rantakokko", "given": "Panu", "initials": "P"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "journal article", "published": "2024-10-08", "journal": {"title": "Environ. Sci. Technol.", "issn": "1520-5851", "volume": "58", "issue": "40", "pages": "17592-17605", "issn-l": "0013-936X"}, "abstract": "For comprehensive chemical exposomics in blood, analytical workflows are evolving through advances in sample preparation and instrumental methods. We hypothesized that gas chromatography-high-resolution mass spectrometry (GC-HRMS) workflows could be enhanced by minimizing lipid coextractives, thereby enabling larger injection volumes and lower matrix interference for improved target sensitivity and nontarget molecular discovery. A simple protocol was developed for small plasma volumes (100-200 \u03bcL) by using isohexane (H) to extract supernatants of acetonitrile-plasma (A-P). The HA-P method was quantitative for a wide range of hydrophobic multiclass target analytes (i.e., log Kow > 3.0), and the extracts were free of major lipids, thereby enabling robust large-volume injections (LVIs; 25 \u03bcL) in long sequences (60-70 h, 70-80 injections) to a GC-Orbitrap HRMS. Without lipid removal, LVI was counterproductive because method sensitivity suffered from the abundant matrix signal, resulting in low ion injection times to the Orbitrap. The median method quantification limit was 0.09 ng/mL (range 0.005-4.83 ng/mL), and good accuracy was shown for a certified reference serum. Applying the method to plasma from a Swedish cohort (n = 32; 100 \u03bcL), 51 of 103 target analytes were detected. Simultaneous nontarget analysis resulted in 112 structural annotations (12.8% annotation rate), and Level 1 identification was achieved for 7 of 8 substances in follow-up confirmations. The HA-P method is potentially scalable for application in cohort studies and is also compatible with many liquid-chromatography-based exposomics workflows.", "doi": "10.1021/acs.est.4c05942", "pmid": "39376097", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11465644"}], "notes": [], "created": "2026-09-23T09:11:17.928Z", "modified": "2026-09-23T09:11:18.015Z"}, {"entity": "publication", "iuid": "f60395474e8144699451cb34b3db1c84", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f60395474e8144699451cb34b3db1c84.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f60395474e8144699451cb34b3db1c84"}}, "title": "Longitudinal Exposomics in a Multiomic Wellness Cohort Reveals Distinctive and Dynamic Environmental Chemical Mixtures in Blood.", "authors": [{"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/871a1de2d146412693d3cfea85e8e293.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F"}, {"family": "Fagerberg", "given": "Linn", "initials": "L"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Bergstr\u00f6m", "given": "G\u00f6ran", "initials": "G"}, {"family": "Martin", "given": "Leah J", "initials": "LJ"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "journal article", "published": "2024-09-17", "journal": {"title": "Environ. Sci. Technol.", "issn": "1520-5851", "volume": "58", "issue": "37", "pages": "16302-16315", "issn-l": "0013-936X"}, "abstract": "Chemical exposomes can now be comprehensively measured in human blood, but knowledge of their variability and longitudinal stability is required for robust application in cohort studies. Here, we applied high-resolution chemical exposomics to plasma of 46 adults, each sampled 6 times over 2 years in a multiomic cohort, resulting in 276 individual exposomes. In addition to quantitative analysis of 83 priority target analytes, we discovered and semiquantified substances that have rarely or never been reported in humans, including personal care products, pesticide transformation products, and polymer additives. Hierarchical cluster analysis for 519 confidently annotated substances revealed unique and distinctive coexposures, including clustered pesticides, poly(ethylene glycols), chlorinated phenols, or natural substances from tea and coffee; interactive heatmaps were publicly deposited to support open exploration of the complex (meta)data. Intraclass correlation coefficients (ICC) for all annotated substances demonstrated the relatively low stability of the exposome compared to that of proteome, microbiome, and endogenous small molecules. Implications are that the chemical exposome must be measured more frequently than other omics in longitudinal studies and four longitudinal exposure types are defined that can be considered in study design. In this small cohort, mixed-effect models nevertheless revealed significant associations between testosterone and perfluoroalkyl substances, demonstrating great potential for longitudinal exposomics in precision health research.", "doi": "10.1021/acs.est.4c05235", "pmid": "39236221", "labels": {"Fredrik Edfors": "", "DDLS Fellow": ""}, "xrefs": [{"db": "pmc", "key": "PMC11411717"}], "notes": [], "created": "2026-09-23T09:08:19.813Z", "modified": "2026-09-23T09:08:19.959Z"}, {"entity": "publication", "iuid": "2152aeac1cf1433491a4076503832c7f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2152aeac1cf1433491a4076503832c7f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2152aeac1cf1433491a4076503832c7f"}}, "title": "Chemical Exposomics in Human Plasma by Lipid Removal and Large Volume Injection Gas Chromatography High-Resolution Mass Spectrometry", "authors": [{"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/871a1de2d146412693d3cfea85e8e293.json"}}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Bergdahl", "given": "Ingvar", "initials": "I"}, {"family": "Rantakokko", "given": "Panu", "initials": "P"}, {"family": "Martin", "given": "Jonathan", "initials": "J"}], "type": "posted-content", "published": "2024-06-02", "journal": {"issn-l": null}, "abstract": null, "doi": "10.26434/chemrxiv-2024-1tpfx", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:03:31.713Z", "modified": "2026-09-23T14:37:08.155Z"}, {"entity": "publication", "iuid": "f6557e4bde204b0eb7e4e7bf78bf8edd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f6557e4bde204b0eb7e4e7bf78bf8edd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f6557e4bde204b0eb7e4e7bf78bf8edd"}}, "title": "Longitudinal Exposomics in a Multiomic Wellness Cohort Reveals Distinctive and Dynamic Environmental Chemical Mixtures in Blood", "authors": [{"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/871a1de2d146412693d3cfea85e8e293.json"}}, {"family": "Edfors", "given": "Fredrik", "initials": "F", "orcid": "0000-0002-0017-7987", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9e213b04f8984c8eb00c68f5bc2f6a36.json"}}, {"family": "Fagerberg", "given": "Linn", "initials": "L", "orcid": "0000-0003-0198-7137", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/abbbf8193d5a4376a5c726768d039439.json"}}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ea446aa574042a295d5f69437402f76.json"}}, {"family": "Bergstr\u00f6m", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0003-4289-5722", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d414b3352a4540a68227ca7eec73cfa6.json"}}, {"family": "Martin", "given": "Leah JM", "initials": "LJ"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "posted-content", "published": "2024-04-17", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.04.14.589329", "pmid": null, "labels": {"Fredrik Edfors": "", "DDLS Fellow": ""}, "xrefs": [], "notes": [], "created": "2026-08-20T10:53:10.468Z", "modified": "2026-09-23T15:38:11.268Z"}, {"entity": "publication", "iuid": "55e6c1ce028245469d4eadf6d51a15b6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/55e6c1ce028245469d4eadf6d51a15b6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/55e6c1ce028245469d4eadf6d51a15b6"}}, "title": "Phospholipid Removal for Enhanced Chemical Exposomics in Human Plasma.", "authors": [{"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H", "orcid": "0000-0002-2422-0492", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/871a1de2d146412693d3cfea85e8e293.json"}}, {"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Bergdahl", "given": "Ingvar A", "initials": "IA"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "journal article", "published": "2023-07-18", "journal": {"title": "Environ. Sci. Technol.", "issn": "1520-5851", "volume": "57", "issue": "28", "pages": "10173-10184", "issn-l": "0013-936X"}, "abstract": "The challenge of chemical exposomics in human plasma is the 1000-fold concentration gap between endogenous substances and environmental pollutants. Phospholipids are the major endogenous small molecules in plasma, thus we validated a chemical exposomics protocol with an optimized phospholipid-removal step prior to targeted and non-targeted liquid chromatography high-resolution mass spectrometry. Increased injection volume with negligible matrix effect permitted sensitive multiclass targeted analysis of 77 priority analytes; median MLOQ = 0.05 ng/mL for 200 \u03bcL plasma. In non-targeted acquisition, mean total signal intensities of non-phospholipids were enhanced 6-fold in positive (max 28-fold) and 4-fold in negative mode (max 58-fold) compared to a control method without phospholipid removal. Moreover, 109 and 28% more non-phospholipid molecular features were detected by exposomics in positive and negative mode, respectively, allowing new substances to be annotated that were non-detectable without phospholipid removal. In individual adult plasma (100 \u03bcL, n = 34), 28 analytes were detected and quantified among 10 chemical classes, and quantitation of per- and polyfluoroalkyl substances (PFAS) was externally validated by independent targeted analysis. Retrospective discovery and semi-quantification of PFAS-precursors was demonstrated, and widespread fenuron exposure is reported in plasma for the first time. The new exposomics method is complementary to metabolomics protocols, relies on open science resources, and can be scaled to support large studies of the exposome.", "doi": "10.1021/acs.est.3c00663", "pmid": "37394749", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10357568"}], "notes": [], "created": "2026-09-23T09:58:40.381Z", "modified": "2026-09-23T10:35:43.457Z"}, {"entity": "publication", "iuid": "987c6f66ae7948a2ad2f0038cc89333e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/987c6f66ae7948a2ad2f0038cc89333e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/987c6f66ae7948a2ad2f0038cc89333e"}}, "title": "Adult Exposure to Di-N-Butyl Phthalate (DBP) Induces Persistent Effects on Testicular Cell Markers and Testosterone Biosynthesis in Mice.", "authors": [{"family": "K\u00e4llsten", "given": "Liselott", "initials": "L", "orcid": "0000-0002-1889-196X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c399772491e84746993efbaaa75acdc5.json"}}, {"family": "Almamoun", "given": "Radwa", "initials": "R", "orcid": "0000-0002-9483-0886", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4fcf01e114de4c6ead50cf39c87383e4.json"}}, {"family": "Pierozan", "given": "Paula", "initials": "P"}, {"family": "Nylander", "given": "Erik", "initials": "E"}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Martin", "given": "Jonathan W", "initials": "JW"}, {"family": "Karlsson", "given": "Oskar", "initials": "O"}], "type": "journal article", "published": "2022-08-05", "journal": {"title": "Int J Mol Sci", "issn": "1422-0067", "volume": "23", "issue": "15", "issn-l": null}, "abstract": "Studies indicate that phthalates are endocrine disruptors affecting reproductive health. One of the most commonly used phthalates, di-n-butyl phthalate (DBP), has been linked with adverse reproductive health outcomes in men, but the mechanisms behind these effects are still poorly understood. Here, adult male mice were orally exposed to DBP (10 or 100 mg/kg/day) for five weeks, and the testis and adrenal glands were collected one week after the last dose, to examine more persistent effects. Quantification of testosterone, androstenedione, progesterone and corticosterone concentrations by liquid chromatography-mass spectrometry showed that testicular testosterone was significantly decreased in both DBP treatment groups, whereas the other steroids were not significantly altered. Western blot analysis of testis revealed that DBP exposure increased the levels of the steroidogenic enzymes CYP11A1, HSD3\u03b22, and CYP17A1, the oxidative stress marker nitrotyrosine, and the luteinizing hormone receptor (LHR). The analysis further demonstrated increased levels of the germ cell marker DAZL, the Sertoli cell markers vimentin and SOX9, and the Leydig cell marker SULT1E1. Overall, the present work provides more mechanistic understanding of how adult DBP exposure can induce effects on the male reproductive system by affecting several key cells and proteins important for testosterone biosynthesis and spermatogenesis, and for the first time shows that these effects persist at least one week after the last dose. It also demonstrates impairment of testosterone biosynthesis at a lower dose than previously reported.", "doi": "10.3390/ijms23158718", "pmid": "35955852", "labels": {"SciLifeLab Fellow": "", "Oskar Karlsson": ""}, "xrefs": [{"db": "pmc", "key": "PMC9369267"}, {"db": "pii", "key": "ijms23158718"}], "notes": [], "created": "2023-06-10T19:59:21.136Z", "modified": "2026-09-23T10:40:22.937Z"}, {"entity": "publication", "iuid": "8c63f5244c8240a8bf249a9203f6dcfb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8c63f5244c8240a8bf249a9203f6dcfb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8c63f5244c8240a8bf249a9203f6dcfb"}}, "title": "Nontarget mass spectrometry and in silico molecular characterization of air pollution from the Indian subcontinent", "authors": [{"family": "Papazian", "given": "Stefano", "initials": "S", "orcid": "0000-0003-2538-8702", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27c56d6b160c42d4b3782d78210e80ff.json"}}, {"family": "D\u2019Agostino", "given": "Lisa A", "initials": "LA"}, {"family": "Sadiktsis", "given": "Ioannis", "initials": "I", "orcid": "0000-0002-1598-7093", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3288e5ef4b1d4df9891108051c9bbc7b.json"}}, {"family": "Froment", "given": "Jean", "initials": "J", "orcid": "0000-0003-4844-2919", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e0ef2b5c0c546cebf2442d26e4e719a.json"}}, {"family": "Bonnefille", "given": "B\u00e9nilde", "initials": "B", "orcid": "0000-0001-5141-7111", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8df2bdf31f21411a90ccc2f9bb6eaf10.json"}}, {"family": "Sdougkou", "given": "Kalliroi", "initials": "K", "orcid": "0000-0001-9463-655X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f07679bf36604909b7533419e618a68e.json"}}, {"family": "Xie", "given": "Hongyu", "initials": "H"}, {"family": "Athanassiadis", "given": "Ioannis", "initials": "I", "orcid": "0000-0001-7069-3330", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0c882db4c39b40a1bd3a8117a14a9389.json"}}, {"family": "Budhavant", "given": "Krishnakant", "initials": "K"}, {"family": "Dasari", "given": "Sanjeev", "initials": "S"}, {"family": "Andersson", "given": "August", "initials": "A", "orcid": "0000-0002-4659-7055", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61532c1fba3c4dd3b1319cb8979b39aa.json"}}, {"family": "Gustafsson", "given": "\u00d6rjan", "initials": "\u00d6"}, {"family": "Martin", "given": "Jonathan W", "initials": "JW", "orcid": "0000-0001-6265-4294", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02199c0d99674e2ea5e96e6589c07a00.json"}}], "type": "journal-article", "published": "2022-02-18", "journal": {"title": "Commun Earth Environ", "issn": "2662-4435", "volume": "3", "issue": "1", "issn-l": null}, "abstract": null, "doi": "10.1038/s43247-022-00365-1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T08:51:53.751Z", "modified": "2026-09-23T08:51:54.043Z"}]}