{"entity": "researcher", "timestamp": "2026-09-25T04:51:10.003Z", "family": "Di Bucchianico", "given": "Sebastiano", "initials": "S", "orcid": "0000-0002-6396-892X", "affiliations": ["Unit of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/efdce7f5d0984e4fa9600afcf0648a5a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/efdce7f5d0984e4fa9600afcf0648a5a"}}, "publications": [{"entity": "publication", "iuid": "d100579dce4b41f6b094b402dcf44a9a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d100579dce4b41f6b094b402dcf44a9a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d100579dce4b41f6b094b402dcf44a9a"}}, "title": "Transcriptome Profiling and Toxicity Following Long-Term, Low Dose Exposure of Human Lung Cells to Ni and NiO Nanoparticles-Comparison with NiCl2.", "authors": [{"family": "Gliga", "given": "Anda R", "initials": "AR"}, {"family": "Di Bucchianico", "given": "Sebastiano", "initials": "S", "orcid": "0000-0002-6396-892X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/efdce7f5d0984e4fa9600afcf0648a5a.json"}}, {"family": "\u00c5kerlund", "given": "Emma", "initials": "E"}, {"family": "Karlsson", "given": "Hanna L", "initials": "HL"}], "type": "journal article", "published": "2020-03-31", "journal": {"title": "Nanomaterials (Basel)", "issn": "2079-4991", "volume": "10", "issue": "4", "issn-l": null}, "abstract": "Production of nickel (Ni) and nickel oxide (NiO) nanoparticles (NPs) leads to a risk of exposure and subsequent health effects. Understanding the toxicological effects and underlying mechanisms using relevant in vitro methods is, therefore, needed. The aim of this study is to explore changes in gene expression using RNA sequencing following long term (six weeks) low dose (0.5 \u00b5g Ni/mL) exposure of human lung cells (BEAS-2B) to Ni and NiO NPs as well as soluble NiCl2. Genotoxicity and cell transformation as well as cellular dose of Ni are also analyzed. Exposure to NiCl2 resulted in the largest number of differentially expressed genes (197), despite limited uptake, suggesting a major role of extracellular receptors and downstream signaling. Gene expression changes for all Ni exposures included genes coding for calcium-binding proteins (S100A14 and S100A2) as well as TIMP3, CCND2, EPCAM, IL4R and DDIT4. Several top enriched pathways for NiCl2 were defined by upregulation of, e.g., interleukin-1A and -1B, as well as Vascular Endothelial Growth Factor A (VEGFA). All Ni exposures caused DNA strand breaks (comet assay), whereas no induction of micronuclei was observed. Taken together, this study provides an insight into Ni-induced toxicity and mechanisms occurring at lower and more realistic exposure levels.", "doi": "10.3390/nano10040649", "pmid": "32244462", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7221965"}, {"db": "pii", "key": "nano10040649"}], "notes": [], "created": "2026-09-23T09:52:20.215Z", "modified": "2026-09-23T10:26:18.748Z"}, {"entity": "publication", "iuid": "5b2f789c186642a899395e352c40552a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5b2f789c186642a899395e352c40552a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5b2f789c186642a899395e352c40552a"}}, "title": "RNA-sequencing reveals long-term effects of silver nanoparticles on human lung cells.", "authors": [{"family": "Gliga", "given": "Anda R", "initials": "AR"}, {"family": "Di Bucchianico", "given": "Sebastiano", "initials": "S", "orcid": "0000-0002-6396-892X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/efdce7f5d0984e4fa9600afcf0648a5a.json"}}, {"family": "Lindvall", "given": "Jessica", "initials": "J"}, {"family": "Fadeel", "given": "Bengt", "initials": "B"}, {"family": "Karlsson", "given": "Hanna L", "initials": "HL"}], "type": "journal article", "published": "2018-04-27", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "8", "issue": "1", "pages": "6668", "issn-l": "2045-2322"}, "abstract": "Despite a considerable focus on the adverse effects of silver nanoparticles (AgNPs) in recent years, studies on the potential long-term effects of AgNPs are scarce. The aim of this study was to explore the effects of AgNPs following repeated low-dose, long-term exposure of human bronchial epithelial cells. To this end, the human BEAS-2B cell line was exposed to 1 \u00b5g/mL AgNPs (10 nm) for 6 weeks followed by RNA-sequencing (RNA-Seq) as well as genome-wide DNA methylation analysis. The transcriptomics analysis showed that a substantial number of genes (1717) were differentially expressed following AgNP exposure whereas only marginal effects on DNA methylation were observed. Downstream analysis of the transcriptomics data identified several affected pathways including the 'fibrosis' and 'epithelial-mesenchymal transition' (EMT) pathway. Subsequently, functional validation studies were performed using AgNPs of two different sizes (10 nm and 75 nm). Both NPs increased collagen deposition, indicative of fibrosis, and induced EMT, as evidenced by an increased invasion index, anchorage independent cell growth, as well as cadherin switching. In conclusion, using a combination of RNA-Seq and functional assays, our study revealed that repeated low-dose, long-term exposure of human BEAS-2B cells to AgNPs is pro-fibrotic, induces EMT and cell transformation.", "doi": "10.1038/s41598-018-25085-5", "pmid": "29703973", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC5923294"}, {"db": "pii", "key": "10.1038/s41598-018-25085-5"}], "notes": [], "created": "2018-12-05T12:41:40.401Z", "modified": "2026-09-23T07:50:21.111Z"}]}