{"entity": "researcher", "timestamp": "2026-08-28T00:11:12.094Z", "family": "Erlandsson", "given": "Ann", "initials": "A", "orcid": "0000-0002-3813-9548", "affiliations": ["Department of Urology, Faculty of Medicine and Health, 59566\u00d6rebro University, \u00d6rebro, Sweden.", "Department of Environmental and Life Sciences/Biology, 101086Karlstad University, Karlstad, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea67c880cba64f3cace05ab8c61c7b88.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea67c880cba64f3cace05ab8c61c7b88"}}, "publications": [{"entity": "publication", "iuid": "66846428d90f432c805f25d18edd39e6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/66846428d90f432c805f25d18edd39e6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/66846428d90f432c805f25d18edd39e6"}}, "title": "Infiltrating immune cells in prostate cancer tissue after androgen deprivation and radiotherapy.", "authors": [{"family": "Erlandsson", "given": "Ann", "initials": "A", "orcid": "0000-0002-3813-9548", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ea67c880cba64f3cace05ab8c61c7b88.json"}}, {"family": "Lundholm", "given": "Marie", "initials": "M"}, {"family": "Watz", "given": "Johan", "initials": "J"}, {"family": "Bergh", "given": "Anders", "initials": "A"}, {"family": "Petrova", "given": "Elitsa", "initials": "E"}, {"family": "Alamdari", "given": "Farhood", "initials": "F"}, {"family": "Helleday", "given": "Thomas", "initials": "T"}, {"family": "Davidsson", "given": "Sabina", "initials": "S"}, {"family": "Andren", "given": "Ove", "initials": "O"}, {"family": "Tarish", "given": "Firas", "initials": "F"}], "type": "journal article", "published": "2023-03-08", "journal": {"title": "Int J Immunopathol Pharmacol", "issn": "2058-7384", "volume": "37", "pages": "3946320231158025", "issn-l": null}, "abstract": "Androgen deprivation therapy (ADT) has long been a cornerstone in treatment of advanced prostate cancer (PCa), and is known to improve the results of radiotherapy (RT) for high-risk disease. The purpose of our study was to use a multiplexed immunohistochemical (mIHC) approach to investigate the infiltration of immune cells in PCa tissue after eight weeks of ADT and/or RT with 10 Gy.\n\nFrom a cohort of 48 patients divided into two treatment arms, we obtained biopsies before and after treatment and used a mIHC method with multispectral imaging to analyze the infiltration of immune cells in tumor stroma and tumor epithelium, focusing on areas with high infiltration.\n\nTumor stroma showed a significantly higher infiltration of immune cells compared to tumor epithelium. The most prominent immune cells were CD20+ B-lymphocytes, followed by CD68+ macrophages, CD8+ cytotoxic T-cells, FOXP3+ regulatory T-cells (Tregs), and T-bet+ Th1-cells. Neoadjuvant ADT followed by RT significantly increased the infiltration of all five immune cells. Numbers of Th1-cells and Tregs significantly increased after single treatment with ADT or RT. In addition, ADT alone increased the number of cytotoxic T-cells and RT increased the number of B-cells.\n\nNeoadjuvant ADT in combination with RT results in a higher inflammatory response compared to RT or ADT alone. The mIHC method may be a useful tool for investigating infiltrating immune cells in PCa biopsies to understand how immunotherapeutic approaches can be combined with current PCa therapies.", "doi": "10.1177/03946320231158025", "pmid": "36880147", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9996739"}], "notes": [], "created": "2026-08-20T12:17:07.681Z", "modified": "2026-08-20T12:17:07.777Z"}]}