{"entity": "researcher", "timestamp": "2026-08-20T21:01:48.220Z", "family": "Claus", "given": "Maren", "initials": "M", "orcid": "0000-0001-8732-0645", "affiliations": ["Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, Dortmund, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/e5fe94feaa4d4ed8929a35838e2c6915.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/e5fe94feaa4d4ed8929a35838e2c6915"}}, "publications": [{"entity": "publication", "iuid": "e8ff69c347d040da88e3f25d7aafc45a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e8ff69c347d040da88e3f25d7aafc45a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e8ff69c347d040da88e3f25d7aafc45a"}}, "title": "NK cells switch from granzyme B to death receptor-mediated cytotoxicity during serial killing.", "authors": [{"family": "Prager", "given": "Isabel", "initials": "I"}, {"family": "Liesche", "given": "Clarissa", "initials": "C"}, {"family": "van Ooijen", "given": "Hanna", "initials": "H"}, {"family": "Urlaub", "given": "Doris", "initials": "D"}, {"family": "Verron", "given": "Quentin", "initials": "Q", "orcid": "0000-0001-5800-4379", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31de7b29ad984949bc1901417bc7ed25.json"}}, {"family": "Sandstr\u00f6m", "given": "Niklas", "initials": "N"}, {"family": "Fasbender", "given": "Frank", "initials": "F"}, {"family": "Claus", "given": "Maren", "initials": "M", "orcid": "0000-0001-8732-0645", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e5fe94feaa4d4ed8929a35838e2c6915.json"}}, {"family": "Eils", "given": "Roland", "initials": "R"}, {"family": "Beaudouin", "given": "Jo\u00ebl", "initials": "J"}, {"family": "\u00d6nfelt", "given": "Bj\u00f6rn", "initials": "B"}, {"family": "Watzl", "given": "Carsten", "initials": "C", "orcid": "0000-0001-5195-0995", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/705dc690627d4843a59d21e3f2b62ea6.json"}}], "type": "journal article", "published": "2019-09-02", "journal": {"title": "J. Exp. Med.", "issn": "1540-9538", "volume": "216", "issue": "9", "pages": "2113-2127", "issn-l": "0022-1007"}, "abstract": "NK cells eliminate virus-infected and tumor cells by releasing cytotoxic granules containing granzyme B (GrzB) or by engaging death receptors that initiate caspase cascades. The orchestrated interplay between both cell death pathways remains poorly defined. Here we simultaneously measure the activities of GrzB and caspase-8 in tumor cells upon contact with human NK cells. We observed that NK cells switch from inducing a fast GrzB-mediated cell death in their first killing events to a slow death receptor-mediated killing during subsequent tumor cell encounters. Target cell contact reduced intracellular GrzB and perforin and increased surface-CD95L in NK cells over time, showing how the switch in cytotoxicity pathways is controlled. Without perforin, NK cells were unable to perform GrzB-mediated serial killing and only killed once via death receptors. In contrast, the absence of CD95 on tumor targets did not impair GrzB-mediated serial killing. This demonstrates that GrzB and death receptor-mediated cytotoxicity are differentially regulated during NK cell serial killing.", "doi": "10.1084/jem.20181454", "pmid": "31270246", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6719417"}, {"db": "pii", "key": "jem.20181454"}], "notes": [], "created": "2026-08-20T09:38:15.463Z", "modified": "2026-08-20T09:38:15.563Z"}]}