{"entity": "researcher", "timestamp": "2026-09-23T14:04:30.713Z", "family": "Ntoufa", "given": "Stavroula", "initials": "S", "orcid": "0000-0003-4155-0140", "affiliations": ["Institute of Applied Biosciences, Centre for Research and Technology Hellas, Thessaloniki 57001, Greece; Hematology Department and Hematopoietic Cell Transplantation Unit, George Papanikolaou Hospital, Thessaloniki 57010, Greece;"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/e4c987a146504098beec10439f4c3b2a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/e4c987a146504098beec10439f4c3b2a"}}, "publications": [{"entity": "publication", "iuid": "1f6b519d771f4d65bab6d3c0a42f54a3", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1f6b519d771f4d65bab6d3c0a42f54a3.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1f6b519d771f4d65bab6d3c0a42f54a3"}}, "title": "B Cell Anergy Modulated by TLR1/2 and the miR-17\u223c92 Cluster Underlies the Indolent Clinical Course of Chronic Lymphocytic Leukemia Stereotyped Subset #4.", "authors": [{"family": "Ntoufa", "given": "Stavroula", "initials": "S", "orcid": "0000-0003-4155-0140", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e4c987a146504098beec10439f4c3b2a.json"}}, {"family": "Papakonstantinou", "given": "Nikos", "initials": "N"}, {"family": "Apollonio", "given": "Benedetta", "initials": "B"}, {"family": "Gounari", "given": "Maria", "initials": "M"}, {"family": "Galigalidou", "given": "Chrysi", "initials": "C"}, {"family": "Fonte", "given": "Eleonora", "initials": "E"}, {"family": "Anagnostopoulos", "given": "Achilles", "initials": "A", "orcid": "0000-0003-4384-9031", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d5cda6cc6fd48a5985120d5e154ff2b.json"}}, {"family": "Belessi", "given": "Chrysoula", "initials": "C"}, {"family": "Muzio", "given": "Marta", "initials": "M"}, {"family": "Ghia", "given": "Paolo", "initials": "P", "orcid": "0000-0003-3750-7342", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/475d35f1a3c446819b2f24be819c2dee.json"}}, {"family": "Stamatopoulos", "given": "Kostas", "initials": "K"}], "type": "journal article", "published": "2016-05-15", "journal": {"title": "J. Immunol.", "issn": "1550-6606", "volume": "196", "issue": "10", "pages": "4410-4417", "issn-l": "0022-1767"}, "abstract": "Chronic lymphocytic leukemia (CLL) patients assigned to stereotyped subset #4 (mutated IGHV4-34/IGKV2-30 BCR Ig) display a particularly indolent disease course. Immunogenetic studies of the clonotypic BCR Ig of CLL subset #4 suggested a resemblance with B cells rendered anergic through chronic autoantigenic stimulation. In this article, we provide experimental evidence that subset #4 CLL cells show low IgG levels, constitutive ERK1/2 activation, and fail to either release intracellular Ca(2+) or activate MAPK signaling after BCR cross-linking, thus displaying a signature of B cell anergy at both biochemical and functional levels. Interestingly, TLR1/2 triggering restored BCR functionality, likely breaching the anergic state, and this was accompanied by induction of the miR-17\u223c92 cluster, whose members target critical BCR-associated molecules, including MAPKs. In conclusion, we demonstrate BCR anergy in CLL subset #4 and implicate TLR signaling and the miR-17\u223c92 cluster in the regulation of the anergic state. This detailed signaling profiling of subset #4 has implications for advanced understanding of the complex regulation of intracellular signaling pathways in CLL, currently a major therapeutic target of the disease.", "doi": "10.4049/jimmunol.1502297", "pmid": "27059597", "labels": [], "xrefs": [{"db": "pii", "key": "jimmunol.1502297"}], "notes": [], "created": "2018-12-05T11:19:04.666Z", "modified": "2026-09-23T11:39:39.331Z"}]}