{"entity": "researcher", "timestamp": "2026-08-20T21:01:23.017Z", "family": "Lemmens", "given": "Bennie B L G", "initials": "BBLG", "orcid": "0000-0001-8051-1676", "affiliations": ["Science for Life Laboratory, Sweden, Tomtebodav\u00e4gen 23A, 17165 Solna, Sweden.", "Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f"}}, "publications": [{"entity": "publication", "iuid": "1d9e77ad5b7d4f4e94772ea47c0d402b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e77ad5b7d4f4e94772ea47c0d402b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e77ad5b7d4f4e94772ea47c0d402b"}}, "title": "Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures", "authors": [{"family": "Hawgood", "given": "Micheal", "initials": "M", "orcid": "0000-0003-3886-7534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4e5a4b28e7e84cd180fd293c1d46d12f.json"}}, {"family": "Uri\u00e9n Gonz\u00e1lez", "given": "Bruno", "initials": "B", "orcid": "0009-0006-8205-6578", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cf8ec549c538474790acf3f77d32928f.json"}}, {"family": "Agostinho", "given": "Ana", "initials": "A", "orcid": "0000-0001-6270-7384", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/16a9e7e5cd0245db94d3b0fb0e1fd58a.json"}}, {"family": "Thiagarajan", "given": "Praghadhesh", "initials": "P", "orcid": "0009-0007-6469-9243", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/80aac1341d8740099c0695390c9c2760.json"}}, {"family": "Yang", "given": "Yiqiu", "initials": "Y", "orcid": "0000-0001-7099-6956", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1d577f598c7749368968b7e0b19f8ef3.json"}}, {"family": "Zhang", "given": "Xue", "initials": "X", "orcid": "0000-0002-5616-3429", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/60978e9fd5194217b437aab7125f66ba.json"}}, {"family": "Giglio", "given": "Giovanni", "initials": "G", "orcid": "0009-0009-0349-3756", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5bd9b83f0ff74ddf8204b0390b59d7d9.json"}}, {"family": "Quijada", "given": "Gemma", "initials": "G", "orcid": "0009-0008-4188-7564", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ac717b155a7f4a30aba612e57fec27a6.json"}}, {"family": "Fonseca", "given": "Matilde", "initials": "M", "orcid": "0000-0002-3809-7632", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1e8b05d5f6074bef86601e89eb725830.json"}}, {"family": "Bartek", "given": "Jiri", "initials": "J", "orcid": "0000-0003-2013-7525", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/288939950c0c4c6393d35c84e5128b5f.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5b0f7dfe5df1495db2a8386c2c5bb4a5.json"}}, {"family": "Lemmens", "given": "Bennie", "initials": "B", "orcid": "0000-0001-8051-1676", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f.json"}}], "type": "posted-content", "published": "2025-02-23", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.02.21.639251", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:01:04.219Z", "modified": "2026-08-20T11:01:04.646Z"}, {"entity": "publication", "iuid": "a4889998c3fa4142848b905506cdd527", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/a4889998c3fa4142848b905506cdd527.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/a4889998c3fa4142848b905506cdd527"}}, "title": "Novel Dihydropteridinone Derivatives As Potent Inhibitors of the Understudied Human Kinases Vaccinia-Related Kinase 1 and Casein Kinase 1\u03b4/\u03b5.", "authors": [{"family": "de Souza Gama", "given": "Fernando H", "initials": "FH"}, {"family": "Dutra", "given": "Luiz A", "initials": "LA", "orcid": "0000-0002-3316-8526", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/54947161b467412cb8b67ea2a9551b5a.json"}}, {"family": "Hawgood", "given": "Michael", "initials": "M", "orcid": "0000-0003-3886-7534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4e5a4b28e7e84cd180fd293c1d46d12f.json"}}, {"family": "Dos Reis", "given": "Caio Vin\u00edcius", "initials": "CV"}, {"family": "Serafim", "given": "Ricardo A M", "initials": "RAM", "orcid": "0000-0003-0614-1798", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/05755073dbbb4a72b9ca9857b7af9ae2.json"}}, {"family": "Ferreira", "given": "Marcos A", "initials": "MA"}, {"family": "Teodoro", "given": "Bruno V M", "initials": "BVM"}, {"family": "Takarada", "given": "J\u00e9ssica Emi", "initials": "JE"}, {"family": "Santiago", "given": "Andr\u00e9 S", "initials": "AS", "orcid": "0000-0002-3973-3370", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e2451d0f47ae4e43a0798ff7b76a5f32.json"}}, {"family": "Balourdas", "given": "Dimitrios-Ilias", "initials": "DI", "orcid": "0000-0002-1790-2268", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bb44c08b0a624b8c827cfc52af368088.json"}}, {"family": "Nilsson", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Urien", "given": "Bruno", "initials": "B"}, {"family": "Almeida", "given": "Vitor M", "initials": "VM"}, {"family": "Gileadi", "given": "Carina", "initials": "C"}, {"family": "Ramos", "given": "Priscila Z", "initials": "PZ"}, {"family": "Salmazo", "given": "Anita", "initials": "A"}, {"family": "Vasconcelos", "given": "Stanley N S", "initials": "SNS"}, {"family": "Cunha", "given": "Micael R", "initials": "MR", "orcid": "0000-0001-5227-6816", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/af9b333b41c7428fb711f6810ce126a7.json"}}, {"family": "Mueller", "given": "Susanne", "initials": "S", "orcid": "0000-0003-2402-4157", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02b49d33d342484a993527458dfacf6b.json"}}, {"family": "Knapp", "given": "Stefan", "initials": "S", "orcid": "0000-0001-5995-6494", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b6025ceada8d460aa28aa3175e423853.json"}}, {"family": "Massirer", "given": "Katlin B", "initials": "KB", "orcid": "0000-0001-6390-2560", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0998db7e84ee48099eab10c7301973cd.json"}}, {"family": "Elkins", "given": "Jonathan M", "initials": "JM", "orcid": "0000-0003-2858-8929", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3492a1a5a1ec490d955b2de5c25bb078.json"}}, {"family": "Gileadi", "given": "Opher", "initials": "O"}, {"family": "Mascarello", "given": "Alessandra", "initials": "A"}, {"family": "Lemmens", "given": "Bennie B L G", "initials": "BBLG", "orcid": "0000-0001-8051-1676", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f.json"}}, {"family": "Guimar\u00e3es", "given": "Cristiano R W", "initials": "CRW"}, {"family": "Azevedo", "given": "Hatylas", "initials": "H", "orcid": "0000-0001-9233-0696", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a22c9763d02f4394b10fb9147048d864.json"}}, {"family": "Cou\u00f1ago", "given": "Rafael M", "initials": "RM", "orcid": "0000-0003-1847-5090", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3508b369daf74b4aa7ec63484b5cffae.json"}}], "type": "journal article", "published": "2024-06-13", "journal": {"title": "J Med Chem", "issn": "1520-4804", "volume": "67", "issue": "11", "pages": "8609-8629", "issn-l": "0022-2623"}, "abstract": "Vaccinia-related kinase 1 (VRK1) and the \u03b4 and \u03b5 isoforms of casein kinase 1 (CK1) are linked to various disease-relevant pathways. However, the lack of tool compounds for these kinases has significantly hampered our understanding of their cellular functions and therapeutic potential. Here, we describe the structure-based development of potent inhibitors of VRK1, a kinase highly expressed in various tumor types and crucial for cell proliferation and genome integrity. Kinome-wide profiling revealed that our compounds also inhibit CK1\u03b4 and CK1\u03b5. We demonstrate that dihydropteridinones 35 and 36 mimic the cellular outcomes of VRK1 depletion. Complementary studies with existing CK1\u03b4 and CK1\u03b5 inhibitors suggest that these kinases may play overlapping roles in cell proliferation and genome instability. Together, our findings highlight the potential of VRK1 inhibition in treating p53-deficient tumors and possibly enhancing the efficacy of existing cancer therapies that target DNA stability or cell division.", "doi": "10.1021/acs.jmedchem.3c02250", "pmid": "38780468", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T08:10:47.717Z", "modified": "2026-08-20T08:10:48.325Z"}, {"entity": "publication", "iuid": "f182dc3e3f73478886336b53059e437c", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f182dc3e3f73478886336b53059e437c.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f182dc3e3f73478886336b53059e437c"}}, "title": "THO complex deficiency impairs DNA double-strand break repair via the RNA surveillance kinase SMG-1.", "authors": [{"family": "Kamp", "given": "Juliette A", "initials": "JA", "orcid": "0000-0002-3901-8852", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7ec4c673e5b741f2b0f1ac979393362c.json"}}, {"family": "Lemmens", "given": "Bennie B L G", "initials": "BBLG", "orcid": "0000-0001-8051-1676", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f.json"}}, {"family": "Romeijn", "given": "Ron J", "initials": "RJ"}, {"family": "Gonz\u00e1lez-Prieto", "given": "Rom\u00e1n", "initials": "R", "orcid": "0000-0001-8997-2321", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/febb70e3527e458c97b39e4c5ee632cc.json"}}, {"family": "Olsen", "given": "Jesper V", "initials": "JV"}, {"family": "Vertegaal", "given": "Alfred C O", "initials": "ACO"}, {"family": "van Schendel", "given": "Robin", "initials": "R", "orcid": "0000-0001-7068-0679", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a580cecb1e014f0a87a79cad4e8e66e1.json"}}, {"family": "Tijsterman", "given": "Marcel", "initials": "M"}], "type": "journal article", "published": "2022-06-24", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "50", "issue": "11", "pages": "6235-6250", "issn-l": "0305-1048"}, "abstract": "The integrity and proper expression of genomes are safeguarded by DNA and RNA surveillance pathways. While many RNA surveillance factors have additional functions in the nucleus, little is known about the incidence and physiological impact of converging RNA and DNA signals. Here, using genetic screens and genome-wide analyses, we identified unforeseen SMG-1-dependent crosstalk between RNA surveillance and DNA repair in living animals. Defects in RNA processing, due to viable THO complex or PNN-1 mutations, induce a shift in DNA repair in dividing and non-dividing tissues. Loss of SMG-1, an ATM/ATR-like kinase central to RNA surveillance by nonsense-mediated decay (NMD), restores DNA repair and radio-resistance in THO-deficient animals. Mechanistically, we find SMG-1 and its downstream target SMG-2/UPF1, but not NMD per se, to suppress DNA repair by non-homologous end-joining in favour of single strand annealing. We postulate that moonlighting proteins create short-circuits in vivo, allowing aberrant RNA to redirect DNA repair.", "doi": "10.1093/nar/gkac472", "pmid": "35670662", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9226523"}, {"db": "pii", "key": "6603656"}], "notes": [], "created": "2026-08-20T09:49:51.889Z", "modified": "2026-08-20T09:49:52.097Z"}, {"entity": "publication", "iuid": "f4d38527fe9a4091a8501ea0b7883f20", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f4d38527fe9a4091a8501ea0b7883f20.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f4d38527fe9a4091a8501ea0b7883f20"}}, "title": "DNA replication and mitotic entry: A brake model for cell cycle progression.", "authors": [{"family": "Lemmens", "given": "Bennie", "initials": "B", "orcid": "0000-0001-8051-1676", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e13fa5660a8d4b18a0ff3df3c263f78f.json"}}, {"family": "Lindqvist", "given": "Arne", "initials": "A", "orcid": "0000-0002-0141-1579", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4e3ecdaacf3d4e6bb5616f0ad3b818ac.json"}}], "type": "journal article", "published": "2019-12-02", "journal": {"title": "J. Cell Biol.", "issn": "1540-8140", "volume": "218", "issue": "12", "pages": "3892-3902", "issn-l": "0021-9525"}, "abstract": "The core function of the cell cycle is to duplicate the genome and divide the duplicated DNA into two daughter cells. These processes need to be carefully coordinated, as cell division before DNA replication is complete leads to genome instability and cell death. Recent observations show that DNA replication, far from being only a consequence of cell cycle progression, plays a key role in coordinating cell cycle activities. DNA replication, through checkpoint kinase signaling, restricts the activity of cyclin-dependent kinases (CDKs) that promote cell division. The S/G2 transition is therefore emerging as a crucial regulatory step to determine the timing of mitosis. Here we discuss recent observations that redefine the coupling between DNA replication and cell division and incorporate these insights into an updated cell cycle model for human cells. We propose a cell cycle model based on a single trigger and sequential releases of three molecular brakes that determine the kinetics of CDK activation.", "doi": "10.1083/jcb.201909032", "pmid": "31712253", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6891093"}, {"db": "pii", "key": "jcb.201909032"}], "notes": [], "created": "2026-08-20T09:37:58.294Z", "modified": "2026-08-20T09:37:58.385Z"}]}