{"entity": "researcher", "timestamp": "2026-08-20T21:35:14.715Z", "family": "Panas", "given": "Marc D", "initials": "MD", "orcid": "0000-0002-7373-0341", "affiliations": ["Division of Virology and Immunology, Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0aacc7004d4b179d37401c8636ac8f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0aacc7004d4b179d37401c8636ac8f"}}, "publications": [{"entity": "publication", "iuid": "f84542e7ea5746209e3f0e441627ad69", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f84542e7ea5746209e3f0e441627ad69.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f84542e7ea5746209e3f0e441627ad69"}}, "title": "SARS-CoV-2 N protein recruits G3BP to double membrane vesicles to promote translation of viral mRNAs.", "authors": [{"family": "Long", "given": "Siwen", "initials": "S"}, {"family": "Guzyk", "given": "Mykhailo", "initials": "M"}, {"family": "Perez Vidakovics", "given": "Laura", "initials": "L", "orcid": "0000-0003-4283-812X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/309ffd38d0f54f26bbe038eb242d7e2d.json"}}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Sun", "given": "Renhua", "initials": "R", "orcid": "0000-0002-8203-4946", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/29b44e08db3a4482a6a40bbda52fe815.json"}}, {"family": "Wang", "given": "Megan", "initials": "M", "orcid": "0000-0003-3000-3539", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6c266ec5cfae49faa110c5b3f34b27ad.json"}}, {"family": "Panas", "given": "Marc D", "initials": "MD", "orcid": "0000-0002-7373-0341", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0aacc7004d4b179d37401c8636ac8f.json"}}, {"family": "Urgard", "given": "Egon", "initials": "E"}, {"family": "Coquet", "given": "Jonathan M", "initials": "JM", "orcid": "0000-0002-5967-4857", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5d8f18ddaf044bdb80d800630d137993.json"}}, {"family": "Merits", "given": "Andres", "initials": "A"}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}, {"family": "McInerney", "given": "Gerald M", "initials": "GM", "orcid": "0000-0003-2257-7241", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/012d1aaf306b43dc89309b45ccc6b649.json"}}], "type": "journal article", "published": "2024-12-05", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "10607", "issn-l": "2041-1723"}, "abstract": "Ras-GTPase-activating protein SH3-domain-binding proteins (G3BP) are critical for the formation of stress granules (SGs) through their RNA- and ribosome-binding properties. SARS-CoV-2 nucleocapsid (N) protein exhibits strong binding affinity for G3BP and inhibits infection-induced SG formation soon after infection. To study the impact of the G3BP-N interaction on viral replication and pathogenesis in detail, we generated a mutant SARS-CoV-2 (RATA) that specifically lacks the G3BP-binding motif in the N protein. RATA triggers a stronger and more persistent SG response in infected cells, showing reduced replication across various cell lines, and greatly reduced pathogenesis in K18-hACE2 transgenic mice. At early times of infection, G3BP and WT N protein strongly colocalise with dsRNA and with non-structural protein 3 (nsp3), a component of the pore complex in double membrane vesicles (DMVs) from which nascent viral RNA emerges. Furthermore, G3BP-N complexes promote highly localized translation of viral mRNAs in the immediate vicinity of the DMVs and thus contribute to efficient viral gene expression and replication. In contrast, G3BP is absent from the DMVs in cells infected with RATA and translation of viral mRNAs is less efficient. This work provides a fuller understanding of the multifunctional roles of G3BP in SARS-CoV-2 infection.", "doi": "10.1038/s41467-024-54996-3", "pmid": "39638802", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11621422"}, {"db": "pii", "key": "10.1038/s41467-024-54996-3"}], "notes": [], "created": "2026-08-20T08:53:44.334Z", "modified": "2026-08-20T08:53:44.554Z"}, {"entity": "publication", "iuid": "9a40e0f6d2394357b80dd513daada491", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9a40e0f6d2394357b80dd513daada491.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9a40e0f6d2394357b80dd513daada491"}}, "title": "Multiple pro-viral effects of the host protein G3BP in SARS-CoV-2 Infection", "authors": [{"family": "McInerney", "given": "Gerald", "initials": "G", "orcid": "0000-0003-2257-7241", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/012d1aaf306b43dc89309b45ccc6b649.json"}}, {"family": "Long", "given": "Siwen", "initials": "S"}, {"family": "Vidakovics", "given": "Laura Perez", "initials": "LP", "orcid": "0000-0003-4283-812X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/309ffd38d0f54f26bbe038eb242d7e2d.json"}}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Sun", "given": "Renhua", "initials": "R"}, {"family": "Wang", "given": "Megan", "initials": "M"}, {"family": "Panas", "given": "Marc", "initials": "M", "orcid": "0000-0002-7373-0341", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0aacc7004d4b179d37401c8636ac8f.json"}}, {"family": "Urgard", "given": "Egon", "initials": "E"}, {"family": "Merits", "given": "Andres", "initials": "A"}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}, {"family": "Guzyk", "given": "Mykhailo", "initials": "M"}, {"family": "Coquet", "given": "Jonathan", "initials": "J"}], "type": "posted-content", "published": "2024-04-08", "journal": {"issn-l": null}, "abstract": null, "doi": "10.21203/rs.3.rs-4145371/v1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T12:59:23.028Z", "modified": "2026-08-20T12:59:23.107Z"}, {"entity": "publication", "iuid": "fc22b72b25834b25bd414d33ad53b8a7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/fc22b72b25834b25bd414d33ad53b8a7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/fc22b72b25834b25bd414d33ad53b8a7"}}, "title": "Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.", "authors": [{"family": "G\u00f6tte", "given": "Benjamin", "initials": "B"}, {"family": "Panas", "given": "Marc D", "initials": "MD", "orcid": "0000-0002-7373-0341", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0aacc7004d4b179d37401c8636ac8f.json"}}, {"family": "Hellstr\u00f6m", "given": "Kirsi", "initials": "K"}, {"family": "Liu", "given": "Lifeng", "initials": "L"}, {"family": "Samreen", "given": "Baila", "initials": "B"}, {"family": "Larsson", "given": "Ola", "initials": "O"}, {"family": "Ahola", "given": "Tero", "initials": "T"}, {"family": "McInerney", "given": "Gerald M", "initials": "GM", "orcid": "0000-0003-2257-7241", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/012d1aaf306b43dc89309b45ccc6b649.json"}}], "type": "journal article", "published": "2019-06-00", "journal": {"title": "PLoS Pathog", "issn": "1553-7374", "volume": "15", "issue": "6", "pages": "e1007842", "issn-l": "1553-7366"}, "abstract": "G3BP-1 and -2 (hereafter referred to as G3BP) are multifunctional RNA-binding proteins involved in stress granule (SG) assembly. Viruses from diverse families target G3BP for recruitment to replication or transcription complexes in order to block SG assembly but also to acquire pro-viral effects via other unknown functions of G3BP. The Old World alphaviruses, including Semliki Forest virus (SFV) and chikungunya virus (CHIKV) recruit G3BP into viral replication complexes, via an interaction between FGDF motifs in the C-terminus of the viral non-structural protein 3 (nsP3) and the NTF2-like domain of G3BP. To study potential proviral roles of G3BP, we used human osteosarcoma (U2OS) cell lines lacking endogenous G3BP generated using CRISPR-Cas9 and reconstituted with a panel of G3BP1 mutants and truncation variants. While SFV replicated with varying efficiency in all cell lines, CHIKV could only replicate in cells expressing G3BP1 variants containing both the NTF2-like and the RGG domains. The ability of SFV to replicate in the absence of G3BP allowed us to study effects of different domains of the protein. We used immunoprecipitation to demonstrate that that both NTF2-like and RGG domains are necessary for the formation a complex between nsP3, G3BP1 and the 40S ribosomal subunit. Electron microscopy of SFV-infected cells revealed that formation of nsP3:G3BP1 complexes via the NTF2-like domain was necessary for clustering of cytopathic vacuoles (CPVs) and that the presence of the RGG domain was necessary for accumulation of electron dense material containing G3BP1 and nsP3 surrounding the CPV clusters. Clustered CPVs also exhibited localised high levels of translation of viral mRNAs as detected by ribopuromycylation staining. These data confirm that G3BP is a ribosomal binding protein and reveal that alphaviral nsP3 uses G3BP to concentrate viral replication complexes and to recruit the translation initiation machinery, promoting the efficient translation of viral mRNAs.", "doi": "10.1371/journal.ppat.1007842", "pmid": "31199850", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6594655"}, {"db": "pii", "key": "PPATHOGENS-D-19-00011"}], "notes": [], "created": "2026-08-20T12:44:38.653Z", "modified": "2026-08-20T12:44:38.744Z"}]}