{"entity": "researcher", "timestamp": "2026-08-20T20:46:24.622Z", "family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "affiliations": ["Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd947633edf949aba9e680e63a1e178b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd947633edf949aba9e680e63a1e178b"}}, "publications": [{"entity": "publication", "iuid": "84b68e9019d34d52aecd219380bdb7bb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/84b68e9019d34d52aecd219380bdb7bb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/84b68e9019d34d52aecd219380bdb7bb"}}, "title": "Single-Cell RNA Analysis Reveals Cell-Intrinsic Functions of CAR T Cells Correlating with Response in a Phase II Study of Lymphoma Patients.", "authors": [{"family": "Sar\u00e9n", "given": "Tina", "initials": "T", "orcid": "0000-0001-5227-6779", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed75a6dcbb9940e49481280af6183d69.json"}}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd947633edf949aba9e680e63a1e178b.json"}}, {"family": "Gammelg\u00e5rd", "given": "Gustav", "initials": "G", "orcid": "0009-0008-5522-0103", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/23c14e3d3bf445a7b3ff9d45eafbc5e1.json"}}, {"family": "L\u00f6vgren", "given": "Tanja", "initials": "T", "orcid": "0000-0003-2170-0682", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4a04a7c6e904ff0945c058fcaa3151d.json"}}, {"family": "Mirabello", "given": "Claudio", "initials": "C", "orcid": "0000-0001-7868-034X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f4ca88bb3cf24e2591c447ec929502e6.json"}}, {"family": "Bj\u00f6rklund", "given": "\u00c5sa K", "initials": "\u00c5K", "orcid": "0000-0003-2224-7090", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1cfd05eeb19640eea44f996699f998f0.json"}}, {"family": "Wikstr\u00f6m", "given": "Kristina", "initials": "K", "orcid": "0009-0000-1027-2534", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1c1933e2ca1f494998c1681623af5ce9.json"}}, {"family": "Hashemi", "given": "Jamileh", "initials": "J", "orcid": "0000-0002-4997-3765", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2e89291626e4f3eb73835d352bf67f4.json"}}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}}, {"family": "Ahlstr\u00f6m", "given": "H\u00e5kan", "initials": "H", "orcid": "0000-0002-8701-969X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7e0504d2b7e14415b7c20229df25c0aa.json"}}, {"family": "Amini", "given": "Rose-Marie", "initials": "RM", "orcid": "0000-0003-0901-5252", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ae2f974dbafa4cdbbe268c635adcd278.json"}}, {"family": "Hagberg", "given": "Hans", "initials": "H", "orcid": "0000-0001-7855-2452", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f525112ab8e94ce790be483b56d7cbd6.json"}}, {"family": "Loskog", "given": "Angelica", "initials": "A", "orcid": "0000-0001-8583-6138", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/572e5b01b3bd48779252b9020732cb09.json"}}, {"family": "Enblad", "given": "Gunilla", "initials": "G", "orcid": "0000-0002-0594-724X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2a285fb437854e2c998356b1a3124416.json"}}, {"family": "Essand", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9725-0422", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a2e56cdd77634c01ab6d98dea8c60430.json"}}], "type": "journal article", "published": "2023-10-13", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "29", "issue": "20", "pages": "4139-4152", "issn-l": "1078-0432"}, "abstract": "Although CD19 chimeric antigen receptor T cells (CAR-T) therapy has shown remarkable success in B-cell malignancies, a substantial fraction of patients do not obtain a long-term clinical response. This could be influenced by the quality of the individual CAR-T infusion product. To shed some light on this, clinical outcome was correlated to characteristics of CAR-T infusion products.\n\nIn this phase II study, patients with B-cell lymphoma (n = 23) or leukemia (n = 1) received one or two infusions of third-generation CD19-directed CAR-Ts (2 \u00d7 108/m2). The clinical trial was registered at clinicaltrials.gov: NCT03068416. We investigated the transcriptional profile of individual CD19 CAR-T infusion products using targeted single-cell RNA sequencing and multicolor flow cytometry.\n\nTwo CAR-T infusions were not better than one in the settings used in this study. As for the CAR-T infusion products, we found that effector-like CD8+CAR-Ts with a high polyfunctionality, high cytotoxic and cytokine production profile, and low dysfunctional signature were associated with clinical response. An extended ex vivo expansion time during CAR-T manufacturing negatively influenced the proportion of effector CD8+CAR-Ts in the infusion product.\n\nWe identified cell-intrinsic characteristics of effector CD8+CAR-Ts correlating with response that could be used as an indicator for clinical outcome. The results in the study also serve as a guide to CAR-T manufacturing practices.", "doi": "10.1158/1078-0432.CCR-23-0178", "pmid": "37540566", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10570681"}, {"db": "pii", "key": "728314"}, {"db": "ClinicalTrials.gov", "key": "NCT03068416"}, {"db": "figshare", "key": "20208764"}], "notes": [], "created": "2026-08-20T12:12:20.782Z", "modified": "2026-08-20T12:12:21.097Z"}, {"entity": "publication", "iuid": "43981b7005d4408b970786e4ef79a2af", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/43981b7005d4408b970786e4ef79a2af.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/43981b7005d4408b970786e4ef79a2af"}}, "title": "CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers.", "authors": [{"family": "Jin", "given": "Chuan", "initials": "C"}, {"family": "Ma", "given": "Jing", "initials": "J"}, {"family": "Ramachandran", "given": "Mohanraj", "initials": "M", "orcid": "0000-0003-2685-0575", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dd947633edf949aba9e680e63a1e178b.json"}}, {"family": "Yu", "given": "Di", "initials": "D", "orcid": "0000-0002-8636-0351", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/80a3098e7db143a5b76d27912d59852f.json"}}, {"family": "Essand", "given": "Magnus", "initials": "M", "orcid": "0000-0002-9725-0422", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a2e56cdd77634c01ab6d98dea8c60430.json"}}], "type": "journal article", "published": "2022-07-00", "journal": {"title": "Nat Biomed Eng", "issn": "2157-846X", "volume": "6", "issue": "7", "pages": "830-841", "issn-l": null}, "abstract": "Chimeric antigen receptor T cells (CAR T cells) are effective against haematologic malignancies. However, in solid tumours, their potency is hampered by local immunosuppression and by the heterogeneous expression of the antigen that the CAR targets. Here we show that CAR T cells expressing a pluripotent pro-inflammatory neutrophil-activating protein (NAP) from Helicobacter pylori trigger endogenous bystander T-cell responses against solid cancers. In mice with subcutaneous murine pancreatic ductal adenocarcinomas, neuroblastomas or colon carcinomas, CAR(NAP) T cells led to slower tumour growth and higher survival rates than conventional mouse CAR T cells, regardless of target antigen, tumour type and host haplotype. In tumours with heterogeneous antigen expression, NAP secretion induced the formation of an immunologically 'hot' microenvironment that supported dendritic cell maturation and bystander responses, as indicated by epitope spreading and infiltration of cytotoxic CD8+ T cells targeting tumour-associated antigens other than the CAR-targeted antigen. CAR T cells armed with NAP neither increased off-tumour toxicity nor hampered the efficacy of CAR T cells, and hence may have advantageous translational potential.", "doi": "10.1038/s41551-022-00875-5", "pmid": "35379957", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9288934"}, {"db": "pii", "key": "10.1038/s41551-022-00875-5"}], "notes": [], "created": "2026-08-20T08:54:49.254Z", "modified": "2026-08-20T08:54:49.396Z"}]}