{"entity": "researcher", "timestamp": "2026-08-20T21:12:31.309Z", "family": "Marques", "given": "Carolina", "initials": "C", "orcid": "0000-0002-8308-5630", "affiliations": ["Seve Ballesteros Foundation Brain Tumor group, CNIO, Madrid, Spain."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc65829f36d243dc94f21a4650ed9c27.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc65829f36d243dc94f21a4650ed9c27"}}, "publications": [{"entity": "publication", "iuid": "3dd9c7165db3452f83259f8880803177", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3dd9c7165db3452f83259f8880803177.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3dd9c7165db3452f83259f8880803177"}}, "title": "NF1 regulates mesenchymal glioblastoma plasticity and aggressiveness through the AP-1 transcription factor FOSL1.", "authors": [{"family": "Marques", "given": "Carolina", "initials": "C", "orcid": "0000-0002-8308-5630", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc65829f36d243dc94f21a4650ed9c27.json"}}, {"family": "Unterkircher", "given": "Thomas", "initials": "T"}, {"family": "Kroon", "given": "Paula", "initials": "P"}, {"family": "Oldrini", "given": "Barbara", "initials": "B"}, {"family": "Izzo", "given": "Annalisa", "initials": "A"}, {"family": "Dramaretska", "given": "Yuliia", "initials": "Y"}, {"family": "Ferrarese", "given": "Roberto", "initials": "R"}, {"family": "Kling", "given": "Eva", "initials": "E"}, {"family": "Schnell", "given": "Oliver", "initials": "O"}, {"family": "Nelander", "given": "Sven", "initials": "S", "orcid": "0000-0003-1758-1262", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f7491aab21f419dbe39276d1ef80077.json"}}, {"family": "Wagner", "given": "Erwin F", "initials": "EF"}, {"family": "Bakiri", "given": "Latifa", "initials": "L"}, {"family": "Gargiulo", "given": "Gaetano", "initials": "G"}, {"family": "Carro", "given": "Maria Stella", "initials": "MS", "orcid": "0000-0002-8570-7691", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0ea3d48cbe1849c5add8bda6b52b701f.json"}}, {"family": "Squatrito", "given": "Massimo", "initials": "M", "orcid": "0000-0002-4593-3790", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/87b7175c8a73474ba07befbf474200fc.json"}}], "type": "journal article", "published": "2021-08-17", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "10", "issn-l": "2050-084X"}, "abstract": "The molecular basis underlying glioblastoma (GBM) heterogeneity and plasticity is not fully understood. Using transcriptomic data of human patient-derived brain tumor stem cell lines (BTSCs), classified based on GBM-intrinsic signatures, we identify the AP-1 transcription factor FOSL1 as a key regulator of the mesenchymal (MES) subtype. We provide a mechanistic basis to the role of the neurofibromatosis type 1 gene (NF1), a negative regulator of the RAS/MAPK pathway, in GBM mesenchymal transformation through the modulation of FOSL1 expression. Depletion of FOSL1 in NF1-mutant human BTSCs and Kras-mutant mouse neural stem cells results in loss of the mesenchymal gene signature and reduction in stem cell properties and in vivo tumorigenic potential. Our data demonstrate that FOSL1 controls GBM plasticity and aggressiveness in response to NF1 alterations.", "doi": "10.7554/eLife.64846", "pmid": "34399888", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8370767"}, {"db": "pii", "key": "64846"}, {"db": "GEO", "key": "GSE137310"}, {"db": "GEO", "key": "GSE138010"}, {"db": "GEO", "key": "GSE119834"}, {"db": "GEO", "key": "GSE67089"}, {"db": "GEO", "key": "GSE8049"}, {"db": "GEO", "key": "GSE49161"}], "notes": [], "created": "2026-08-20T13:53:24.659Z", "modified": "2026-08-20T13:53:24.751Z"}, {"entity": "publication", "iuid": "ad4df49d4b064e89865aac6a2484a455", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ad4df49d4b064e89865aac6a2484a455.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ad4df49d4b064e89865aac6a2484a455"}}, "title": "Author response: NF1 regulates mesenchymal glioblastoma plasticity and aggressiveness through the AP-1 transcription factor FOSL1", "authors": [{"family": "Marques", "given": "Carolina", "initials": "C", "orcid": "0000-0002-8308-5630", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc65829f36d243dc94f21a4650ed9c27.json"}}, {"family": "Unterkircher", "given": "Thomas", "initials": "T"}, {"family": "Kroon", "given": "Paula", "initials": "P"}, {"family": "Oldrini", "given": "Barbara", "initials": "B"}, {"family": "Izzo", "given": "Annalisa", "initials": "A"}, {"family": "Dramaretska", "given": "Yuliia", "initials": "Y"}, {"family": "Ferrarese", "given": "Roberto", "initials": "R"}, {"family": "Kling", "given": "Eva", "initials": "E"}, {"family": "Schnell", "given": "Oliver", "initials": "O"}, {"family": "Nelander", "given": "Sven", "initials": "S", "orcid": "0000-0003-1758-1262", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f7491aab21f419dbe39276d1ef80077.json"}}, {"family": "Wagner", "given": "Erwin F", "initials": "EF"}, {"family": "Bakiri", "given": "Latifa", "initials": "L"}, {"family": "Gargiulo", "given": "Gaetano", "initials": "G"}, {"family": "Carro", "given": "Maria Stella", "initials": "MS", "orcid": "0000-0002-8570-7691", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0ea3d48cbe1849c5add8bda6b52b701f.json"}}, {"family": "Squatrito", "given": "Massimo", "initials": "M", "orcid": "0000-0002-4593-3790", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/87b7175c8a73474ba07befbf474200fc.json"}}], "type": "peer-review", "published": "2021-06-11", "journal": {"issn-l": null}, "abstract": null, "doi": "10.7554/elife.64846.sa2", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:53:26.636Z", "modified": "2026-08-20T13:53:26.709Z"}, {"entity": "publication", "iuid": "dffb9a243b2544118016c0930cbc08bd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/dffb9a243b2544118016c0930cbc08bd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/dffb9a243b2544118016c0930cbc08bd"}}, "title": "The Irradiated Brain Microenvironment Supports Glioma Stemness and Survival via Astrocyte-Derived Transglutaminase 2.", "authors": [{"family": "Berg", "given": "Tracy J", "initials": "TJ"}, {"family": "Marques", "given": "Carolina", "initials": "C", "orcid": "0000-0002-8308-5630", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dc65829f36d243dc94f21a4650ed9c27.json"}}, {"family": "Pantazopoulou", "given": "Vasiliki", "initials": "V", "orcid": "0000-0002-5965-5795", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b4e8f1e6335241148a153f34611d9145.json"}}, {"family": "Johansson", "given": "Elinn", "initials": "E"}, {"family": "von Stedingk", "given": "Kristoffer", "initials": "K"}, {"family": "Lindgren", "given": "David", "initials": "D"}, {"family": "Jeannot", "given": "Pauline", "initials": "P", "orcid": "0000-0003-0158-440X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/167869db86f0456fbb7a535ae8d89b35.json"}}, {"family": "Pietras", "given": "Elin J", "initials": "EJ", "orcid": "0000-0002-5783-9347", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9d48aa66cee48b9828c0ac374c75362.json"}}, {"family": "Bergstr\u00f6m", "given": "Tobias", "initials": "T", "orcid": "0000-0002-4557-2390", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad8f5ab0560d4b7596ddd5e407b96d8f.json"}}, {"family": "Swartling", "given": "Fredrik J", "initials": "FJ", "orcid": "0000-0002-8460-4367", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e673fa8c86348fb8c1a2ae34ba7ba9a.json"}}, {"family": "Governa", "given": "Valeria", "initials": "V"}, {"family": "Bengzon", "given": "Johan", "initials": "J"}, {"family": "Belting", "given": "Mattias", "initials": "M"}, {"family": "Axelson", "given": "H\u00e5kan", "initials": "H"}, {"family": "Squatrito", "given": "Massimo", "initials": "M"}, {"family": "Pietras", "given": "Alexander", "initials": "A", "orcid": "0000-0002-5783-9347", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f9d48aa66cee48b9828c0ac374c75362.json"}}], "type": "journal article", "published": "2021-04-15", "journal": {"title": "Cancer Res.", "issn": "1538-7445", "volume": "81", "issue": "8", "pages": "2101-2115", "issn-l": "0008-5472"}, "abstract": "The tumor microenvironment plays an essential role in supporting glioma stemness and radioresistance. Following radiotherapy, recurrent gliomas form in an irradiated microenvironment. Here we report that astrocytes, when pre-irradiated, increase stemness and survival of cocultured glioma cells. Tumor-na\u00efve brains increased reactive astrocytes in response to radiation, and mice subjected to radiation prior to implantation of glioma cells developed more aggressive tumors. Extracellular matrix derived from irradiated astrocytes were found to be a major driver of this phenotype and astrocyte-derived transglutaminase 2 (TGM2) was identified as a promoter of glioma stemness and radioresistance. TGM2 levels increased after radiation in vivo and in recurrent human glioma, and TGM2 inhibitors abrogated glioma stemness and survival. These data suggest that irradiation of the brain results in the formation of a tumor-supportive microenvironment. Therapeutic targeting of radiation-induced, astrocyte-derived extracellular matrix proteins may enhance the efficacy of standard-of-care radiotherapy by reducing stemness in glioma. SIGNIFICANCE: These findings presented here indicate that radiotherapy can result in a tumor-supportive microenvironment, the targeting of which may be necessary to overcome tumor cell therapeutic resistance and recurrence. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/81/8/2101/F1.large.jpg.", "doi": "10.1158/0008-5472.CAN-20-1785", "pmid": "33483373", "labels": [], "xrefs": [{"db": "pii", "key": "0008-5472.CAN-20-1785"}], "notes": [], "created": "2026-08-20T12:11:03.084Z", "modified": "2026-08-20T12:11:03.255Z"}]}