{"entity": "researcher", "timestamp": "2026-08-20T21:11:37.685Z", "family": "Koliyadu", "given": "Jayanath", "initials": "J", "orcid": "0000-0002-0245-3842", "affiliations": ["European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.", "Biomedical and X-Ray Physics, Department of Applied Physics, AlbaNova University Center, KTH Royal Institute of Technology, SE-10691, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbd250665f634af0a3643eb5ec8d1f87.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbd250665f634af0a3643eb5ec8d1f87"}}, "publications": [{"entity": "publication", "iuid": "d27199f289414efd9110c67ebae0aa7f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d27199f289414efd9110c67ebae0aa7f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d27199f289414efd9110c67ebae0aa7f"}}, "title": "Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.", "authors": [{"family": "Konold", "given": "Patrick E", "initials": "PE"}, {"family": "Monrroy", "given": "Leonardo", "initials": "L", "orcid": "0000-0001-8852-9424", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7a1a74297e27463bbbb993b02307fb08.json"}}, {"family": "Bellisario", "given": "Alfredo", "initials": "A", "orcid": "0000-0001-5155-4431", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/026e73cf9b2f4f928c0bf8f91d974ec7.json"}}, {"family": "Filipe", "given": "Diogo", "initials": "D"}, {"family": "Adams", "given": "Patrick", "initials": "P"}, {"family": "Alvarez", "given": "Roberto", "initials": "R"}, {"family": "Bean", "given": "Richard", "initials": "R", "orcid": "0000-0001-8151-7439", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a9023f98dc3e413d98da69eb75321bb9.json"}}, {"family": "Bielecki", "given": "Johan", "initials": "J", "orcid": "0000-0002-3012-603X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb981ea4fae1468fbf068f2b4b509c75.json"}}, {"family": "B\u00f3dizs", "given": "Szabolcs", "initials": "S"}, {"family": "Ducrocq", "given": "Gabriel", "initials": "G"}, {"family": "Grubmueller", "given": "Helmut", "initials": "H"}, {"family": "Kirian", "given": "Richard A", "initials": "RA", "orcid": "0000-0001-7197-3086", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8b7cc39ee4614a52a470e386ffd469af.json"}}, {"family": "Kloos", "given": "Marco", "initials": "M"}, {"family": "Koliyadu", "given": "Jayanath C P", "initials": "JCP", "orcid": "0000-0002-0245-3842", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbd250665f634af0a3643eb5ec8d1f87.json"}}, {"family": "Koua", "given": "Faisal H M", "initials": "FHM", "orcid": "0000-0001-8371-9587", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/645de87b1d924a7b9bd5e25aefabbb21.json"}}, {"family": "Larkiala", "given": "Taru", "initials": "T"}, {"family": "Letrun", "given": "Romain", "initials": "R", "orcid": "0000-0002-0569-5193", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d8f34ffb59024c8483d554b06be94457.json"}}, {"family": "Lindsten", "given": "Fredrik", "initials": "F"}, {"family": "Maih\u00f6fer", "given": "Michael", "initials": "M"}, {"family": "Martin", "given": "Andrew V", "initials": "AV", "orcid": "0000-0003-3704-1829", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e0cb70f845043f8a8d482581f97c040.json"}}, {"family": "M\u00e9sz\u00e1ros", "given": "Petra", "initials": "P"}, {"family": "Mutisya", "given": "Jennifer", "initials": "J"}, {"family": "Nimmrich", "given": "Amke", "initials": "A", "orcid": "0000-0003-0425-2101", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/136bad817bdf47a09bc2202cf5eb47e7.json"}}, {"family": "Okamoto", "given": "Kenta", "initials": "K", "orcid": "0000-0002-4858-1196", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f35f8c5f2f1545af82ea621d61ee484d.json"}}, {"family": "Round", "given": "Adam", "initials": "A", "orcid": "0000-0002-0723-8228", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0945f297d3d347388f689f02bf43fd2b.json"}}, {"family": "Sato", "given": "Tokushi", "initials": "T", "orcid": "0000-0003-3155-3487", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9a6ff4a0efb547fe8d1989561009e840.json"}}, {"family": "Valerio", "given": "Joana", "initials": "J"}, {"family": "Westphal", "given": "Daniel", "initials": "D"}, {"family": "Wollter", "given": "August", "initials": "A"}, {"family": "Yenupuri", "given": "Tej Varma", "initials": "TV"}, {"family": "You", "given": "Tong", "initials": "T"}, {"family": "Maia", "given": "Filipe", "initials": "F", "orcid": "0000-0002-2141-438X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a305c750241840a19612dc53e34beacc.json"}}, {"family": "Westenhoff", "given": "Sebastian", "initials": "S", "orcid": "0000-0002-6961-8015", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2349e8a6f0144bcd95a9ee1d87711e9a.json"}}], "type": "journal article", "published": "2024-09-00", "journal": {"title": "Nat. Methods", "issn": "1548-7105", "volume": "21", "issue": "9", "pages": "1608-1611", "issn-l": "1548-7091"}, "abstract": "Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining J\u0251 helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.", "doi": "10.1038/s41592-024-02344-0", "pmid": "38969722", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11399097"}, {"db": "pii", "key": "10.1038/s41592-024-02344-0"}], "notes": [], "created": "2026-08-20T09:03:11.921Z", "modified": "2026-08-20T09:03:12.492Z"}, {"entity": "publication", "iuid": "3cbddbb141294cffa470d8b4f017e95e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3cbddbb141294cffa470d8b4f017e95e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3cbddbb141294cffa470d8b4f017e95e"}}, "title": "Observation of a single protein by ultrafast X-ray diffraction.", "authors": [{"family": "Ekeberg", "given": "Tomas", "initials": "T"}, {"family": "Assalauova", "given": "Dameli", "initials": "D"}, {"family": "Bielecki", "given": "Johan", "initials": "J", "orcid": "0000-0002-3012-603X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb981ea4fae1468fbf068f2b4b509c75.json"}}, {"family": "Boll", "given": "Rebecca", "initials": "R", "orcid": "0000-0001-6286-4064", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/11394aae9c824504bd45759deb2a7a76.json"}}, {"family": "Daurer", "given": "Benedikt J", "initials": "BJ", "orcid": "0000-0002-1887-7551", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/22e1c4b4b7fc427f9069efa4b1486d47.json"}}, {"family": "Eichacker", "given": "Lutz A", "initials": "LA"}, {"family": "Franken", "given": "Linda E", "initials": "LE", "orcid": "0000-0002-3040-8192", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31a6b4279ad742e2b2078fb4b81b0967.json"}}, {"family": "Galli", "given": "Davide E", "initials": "DE", "orcid": "0000-0002-1312-1181", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/70c549226f6d4264850608b3250dd0ba.json"}}, {"family": "Gelisio", "given": "Luca", "initials": "L"}, {"family": "Gumprecht", "given": "Lars", "initials": "L"}, {"family": "Gunn", "given": "Laura H", "initials": "LH"}, {"family": "Hajdu", "given": "Janos", "initials": "J"}, {"family": "Hartmann", "given": "Robert", "initials": "R", "orcid": "0000-0003-1839-5365", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/11ce7ef4cc4f4c9f93d775dca73b40df.json"}}, {"family": "Hasse", "given": "Dirk", "initials": "D"}, {"family": "Ignatenko", "given": "Alexandr", "initials": "A"}, {"family": "Koliyadu", "given": "Jayanath", "initials": "J", "orcid": "0000-0002-0245-3842", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbd250665f634af0a3643eb5ec8d1f87.json"}}, {"family": "Kulyk", "given": "Olena", "initials": "O"}, {"family": "Kurta", "given": "Ruslan", "initials": "R"}, {"family": "Kuster", "given": "Markus", "initials": "M", "orcid": "0000-0002-4361-7102", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fe962434b1de4f538c4752079e817360.json"}}, {"family": "Lugmayr", "given": "Wolfgang", "initials": "W", "orcid": "0000-0001-8501-8637", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/82a88df5177d400e969989f8f41b42bc.json"}}, {"family": "L\u00fcbke", "given": "Jannik", "initials": "J"}, {"family": "Mancuso", "given": "Adrian P", "initials": "AP"}, {"family": "Mazza", "given": "Tommaso", "initials": "T"}, {"family": "Nettelblad", "given": "Carl", "initials": "C", "orcid": "0000-0003-0458-6902", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da4567d9202441e29412c827729e7134.json"}}, {"family": "Ovcharenko", "given": "Yevheniy", "initials": "Y"}, {"family": "Rivas", "given": "Daniel E", "initials": "DE", "orcid": "0000-0002-7540-4902", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e69eed8791a54c81a57a810a258cb787.json"}}, {"family": "Rose", "given": "Max", "initials": "M", "orcid": "0000-0001-5723-0884", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cdc0508385e946bba69d99a356a04f28.json"}}, {"family": "Samanta", "given": "Amit K", "initials": "AK"}, {"family": "Schmidt", "given": "Philipp", "initials": "P", "orcid": "0000-0003-1980-6998", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32ad7c99f9004741a7347fab1a0909f7.json"}}, {"family": "Sobolev", "given": "Egor", "initials": "E", "orcid": "0000-0003-2478-5685", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8d276c61bf24456f90c0c4a4890c3dd6.json"}}, {"family": "Timneanu", "given": "Nicusor", "initials": "N"}, {"family": "Usenko", "given": "Sergey", "initials": "S", "orcid": "0000-0001-5202-5267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a7752b794dac4a2ead20312996e4ae87.json"}}, {"family": "Westphal", "given": "Daniel", "initials": "D"}, {"family": "Wollweber", "given": "Tamme", "initials": "T"}, {"family": "Worbs", "given": "Lena", "initials": "L"}, {"family": "Xavier", "given": "Paul Lourdu", "initials": "PL", "orcid": "0000-0001-5132-2999", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4541dbeb59ad44f6aaa167fb4d4a1e89.json"}}, {"family": "Yousef", "given": "Hazem", "initials": "H"}, {"family": "Ayyer", "given": "Kartik", "initials": "K", "orcid": "0000-0002-6881-564X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ed596a62143046adb46e0484fcd454da.json"}}, {"family": "Chapman", "given": "Henry N", "initials": "HN", "orcid": "0000-0002-4655-1743", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c661cbe64a6143ac8c44594ce69931a5.json"}}, {"family": "Sellberg", "given": "Jonas A", "initials": "JA", "orcid": "0000-0003-2793-5052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/86c7efd806b5436296298d6243778574.json"}}, {"family": "Seuring", "given": "Carolin", "initials": "C", "orcid": "0000-0003-1000-0859", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2f355ad3b6894b11896335c578ec956c.json"}}, {"family": "Vartanyants", "given": "Ivan A", "initials": "IA", "orcid": "0000-0002-0340-8234", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a3828d7e3b604adc92e8d9b74b47896d.json"}}, {"family": "K\u00fcpper", "given": "Jochen", "initials": "J", "orcid": "0000-0003-4395-9345", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4418443871e14f17ada6b50ac02f1232.json"}}, {"family": "Meyer", "given": "Michael", "initials": "M", "orcid": "0000-0002-1444-6770", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fdcd87f28ccc40d19b0345fc1a41c08a.json"}}, {"family": "Maia", "given": "Filipe R N C", "initials": "FRNC"}], "type": "journal article", "published": "2024-01-12", "journal": {"title": "Light Sci Appl", "issn": "2047-7538", "volume": "13", "issue": "1", "pages": "15", "issn-l": null}, "abstract": "The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to theory, the extremely intense pulses provide sufficient signal to dispense with using crystals as an amplifier, and the ultrashort pulse duration permits capturing the diffraction data before the sample inevitably explodes. This was first demonstrated on biological samples a decade ago on the giant mimivirus. Since then, a large collaboration has been pushing the limit of the smallest sample that can be imaged. The ability to capture snapshots on the timescale of atomic vibrations, while keeping the sample at room temperature, may allow probing the entire conformational phase space of macromolecules. Here we show the first observation of an X-ray diffraction pattern from a single protein, that of Escherichia coli GroEL which at 14 nm in diameter is the smallest biological sample ever imaged by X-rays, and demonstrate that the concept of diffraction before destruction extends to single proteins. From the pattern, it is possible to determine the approximate orientation of the protein. Our experiment demonstrates the feasibility of ultrafast imaging of single proteins, opening the way to single-molecule time-resolved studies on the femtosecond timescale.", "doi": "10.1038/s41377-023-01352-7", "pmid": "38216563", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10786860"}, {"db": "pii", "key": "10.1038/s41377-023-01352-7"}], "notes": [], "created": "2026-08-20T08:47:31.976Z", "modified": "2026-08-20T08:47:56.394Z"}, {"entity": "publication", "iuid": "3b6918deed394fb1a8a2d3d7877e3f9e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3b6918deed394fb1a8a2d3d7877e3f9e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3b6918deed394fb1a8a2d3d7877e3f9e"}}, "title": "3D-printed sheet jet for stable megahertz liquid sample delivery at X-ray free-electron lasers.", "authors": [{"family": "Konold", "given": "Patrick E", "initials": "PE"}, {"family": "You", "given": "Tong", "initials": "T"}, {"family": "Bielecki", "given": "Johan", "initials": "J"}, {"family": "Valerio", "given": "Joana", "initials": "J", "orcid": "0000-0001-5931-0925", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/38ebd6b25c87437095a47c1b7bf44ecd.json"}}, {"family": "Kloos", "given": "Marco", "initials": "M", "orcid": "0000-0002-8745-9740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/30f60d7edeaa451b9c6862c3d797814c.json"}}, {"family": "Westphal", "given": "Daniel", "initials": "D", "orcid": "0000-0001-8735-0369", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8b65c15572f045bebbd2b3e54d3361ca.json"}}, {"family": "Bellisario", "given": "Alfredo", "initials": "A", "orcid": "0000-0001-5155-4431", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/026e73cf9b2f4f928c0bf8f91d974ec7.json"}}, {"family": "Varma Yenupuri", "given": "Tej", "initials": "T"}, {"family": "Wollter", "given": "August", "initials": "A"}, {"family": "Koliyadu", "given": "Jayanath C P", "initials": "JCP", "orcid": "0000-0002-0245-3842", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dbd250665f634af0a3643eb5ec8d1f87.json"}}, {"family": "Koua", "given": "Faisal H M", "initials": "FHM", "orcid": "0000-0001-8371-9587", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/645de87b1d924a7b9bd5e25aefabbb21.json"}}, {"family": "Letrun", "given": "Romain", "initials": "R", "orcid": "0000-0002-0569-5193", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d8f34ffb59024c8483d554b06be94457.json"}}, {"family": "Round", "given": "Adam", "initials": "A", "orcid": "0000-0002-0723-8228", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0945f297d3d347388f689f02bf43fd2b.json"}}, {"family": "Sato", "given": "Tokushi", "initials": "T", "orcid": "0000-0003-3155-3487", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9a6ff4a0efb547fe8d1989561009e840.json"}}, {"family": "M\u00e9sz\u00e1ros", "given": "Petra", "initials": "P"}, {"family": "Monrroy", "given": "Leonardo", "initials": "L", "orcid": "0000-0001-8852-9424", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7a1a74297e27463bbbb993b02307fb08.json"}}, {"family": "Mutisya", "given": "Jennifer", "initials": "J"}, {"family": "B\u00f3dizs", "given": "Szabolcs", "initials": "S"}, {"family": "Larkiala", "given": "Taru", "initials": "T", "orcid": "0009-0004-4180-1311", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4210b0e1f3984d38a7efb5eb11e47177.json"}}, {"family": "Nimmrich", "given": "Amke", "initials": "A", "orcid": "0000-0003-0425-2101", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/136bad817bdf47a09bc2202cf5eb47e7.json"}}, {"family": "Alvarez", "given": "Roberto", "initials": "R"}, {"family": "Adams", "given": "Patrick", "initials": "P"}, {"family": "Bean", "given": "Richard", "initials": "R"}, {"family": "Ekeberg", "given": "Tomas", "initials": "T", "orcid": "0000-0003-0092-2104", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba9bc0d5dda542ef9f69da623557a977.json"}}, {"family": "Kirian", "given": "Richard A", "initials": "RA", "orcid": "0000-0001-7197-3086", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8b7cc39ee4614a52a470e386ffd469af.json"}}, {"family": "Martin", "given": "Andrew V", "initials": "AV"}, {"family": "Westenhoff", "given": "Sebastian", "initials": "S"}, {"family": "Maia", "given": "Filipe R N C", "initials": "FRNC"}], "type": "journal article", "published": "2023-11-01", "journal": {"title": "IUCrJ", "issn": "2052-2525", "volume": "10", "issue": "Pt 6", "pages": "662-670", "issn-l": "2052-2525"}, "abstract": "X-ray free-electron lasers (XFELs) can probe chemical and biological reactions as they unfold with unprecedented spatial and temporal resolution. A principal challenge in this pursuit involves the delivery of samples to the X-ray interaction point in such a way that produces data of the highest possible quality and with maximal efficiency. This is hampered by intrinsic constraints posed by the light source and operation within a beamline environment. For liquid samples, the solution typically involves some form of high-speed liquid jet, capable of keeping up with the rate of X-ray pulses. However, conventional jets are not ideal because of radiation-induced explosions of the jet, as well as their cylindrical geometry combined with the X-ray pointing instability of many beamlines which causes the interaction volume to differ for every pulse. This complicates data analysis and contributes to measurement errors. An alternative geometry is a liquid sheet jet which, with its constant thickness over large areas, eliminates the problems related to X-ray pointing. Since liquid sheets can be made very thin, the radiation-induced explosion is reduced, boosting their stability. These are especially attractive for experiments which benefit from small interaction volumes such as fluctuation X-ray scattering and several types of spectroscopy. Although their use has increased for soft X-ray applications in recent years, there has not yet been wide-scale adoption at XFELs. Here, gas-accelerated liquid sheet jet sample injection is demonstrated at the European XFEL SPB/SFX nano focus beamline. Its performance relative to a conventional liquid jet is evaluated and superior performance across several key factors has been found. This includes a thickness profile ranging from hundreds of nanometres to 60 nm, a fourfold increase in background stability and favorable radiation-induced explosion dynamics at high repetition rates up to 1.13 MHz. Its minute thickness also suggests that ultrafast single-particle solution scattering is a possibility.", "doi": "10.1107/S2052252523007972", "pmid": "37721770", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10619454"}, {"db": "pii", "key": "S2052252523007972"}], "notes": [], "created": "2026-08-20T11:16:10.351Z", "modified": "2026-08-20T11:16:10.636Z"}]}