{"entity": "researcher", "timestamp": "2026-08-23T22:15:27.592Z", "family": "\u00d6lander", "given": "Magnus", "initials": "M", "orcid": "0000-0002-4502-8184", "affiliations": ["Department of Pharmacy, Uppsala University, Uppsala, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb"}}, "publications": [{"entity": "publication", "iuid": "8058bb54e50545e596432edee0714edc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8058bb54e50545e596432edee0714edc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8058bb54e50545e596432edee0714edc"}}, "title": "Hepatocyte size fractionation allows dissection of human liver zonation.", "authors": [{"family": "\u00d6lander", "given": "Magnus", "initials": "M", "orcid": "0000-0002-4502-8184", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb.json"}}, {"family": "Wegler", "given": "Christine", "initials": "C"}, {"family": "Fl\u00f6rkemeier", "given": "Inken", "initials": "I"}, {"family": "Treyer", "given": "Andrea", "initials": "A"}, {"family": "Handin", "given": "Niklas", "initials": "N"}, {"family": "Pedersen", "given": "Jenny M", "initials": "JM"}, {"family": "Vildhede", "given": "Anna", "initials": "A"}, {"family": "Mateus", "given": "Andr\u00e9", "initials": "A"}, {"family": "LeCluyse", "given": "Edward L", "initials": "EL"}, {"family": "Urdzik", "given": "Jozef", "initials": "J"}, {"family": "Artursson", "given": "Per", "initials": "P", "orcid": "0000-0002-3708-7395", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9908209a413a4088b6bf44a924d27319.json"}}], "type": "journal article", "published": "2021-08-00", "journal": {"title": "J. Cell. Physiol.", "issn": "1097-4652", "volume": "236", "issue": "8", "pages": "5885-5894", "issn-l": "0021-9541"}, "abstract": "Human hepatocytes show marked differences in cell size, gene expression, and function throughout the liver lobules, an arrangement termed liver zonation. However, it is not clear if these zonal size differences, and the associated phenotypic differences, are retained in isolated human hepatocytes, the \"gold standard\" for in vitro studies of human liver function. Here, we therefore explored size differences among isolated human hepatocytes and investigated whether separation by size can be used to study liver zonation in vitro. We used counterflow centrifugal elutriation to separate cells into different size fractions and analyzed them with label-free quantitative proteomics, which revealed an enrichment of 151 and 758 proteins (out of 5163) in small and large hepatocytes, respectively. Further analysis showed that protein abundances in different hepatocyte size fractions recapitulated the in vivo expression patterns of previously described zonal markers and biological processes. We also found that the expression of zone-specific cytochrome P450 enzymes correlated with their metabolic activity in the different fractions. In summary, our results show that differences in hepatocyte size matches zonal expression patterns, and that our size fractionation approach can be used to study zone-specific liver functions in vitro.", "doi": "10.1002/jcp.30273", "pmid": "33452735", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:33:59.319Z", "modified": "2026-08-20T06:33:59.425Z"}, {"entity": "publication", "iuid": "6a61abc6faca43b49e667d1d00a10eaa", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6a61abc6faca43b49e667d1d00a10eaa.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6a61abc6faca43b49e667d1d00a10eaa"}}, "title": "Cell-type-resolved proteomic analysis of the human liver.", "authors": [{"family": "\u00d6lander", "given": "Magnus", "initials": "M", "orcid": "0000-0002-4502-8184", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb.json"}}, {"family": "Wi\u015bniewski", "given": "Jacek R", "initials": "JR"}, {"family": "Artursson", "given": "Per", "initials": "P", "orcid": "0000-0002-3708-7395", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9908209a413a4088b6bf44a924d27319.json"}}], "type": "journal article", "published": "2020-07-00", "journal": {"title": "Liver Int.", "issn": "1478-3231", "volume": "40", "issue": "7", "pages": "1770-1780", "issn-l": "1478-3223"}, "abstract": "The human liver functions through a complex interplay between parenchymal and non-parenchymal cells. Mass spectrometry-based proteomic analysis of intact tissue has provided an in-depth view of the human liver proteome. However, the predominance of parenchymal cells (hepatocytes) means that the total tissue proteome mainly reflects hepatocyte expression. Here we therefore set out to analyse the proteomes of the major parenchymal and non-parenchymal cell types in the human liver.\n\nWe applied quantitative label-free proteomic analysis on the major cell types of the human liver: hepatocytes, liver endothelial cells, Kupffer cells and hepatic stellate cells.\n\nWe identified 9791 proteins, revealing distinct protein expression profiles across cell types, whose in vivo relevance was shown by the presence of cell-type-specific proteins. Analysis of proteins related to the immune system indicated that mechanisms of immune-mediated liver injury include the involvement of several cell types. Furthermore, in-depth investigation of proteins related to the absorption, distribution, metabolism, excretion and toxicity (ADMET) of xenobiotics showed that ADMET-related tasks are not exclusively confined to hepatocytes, and that non-parenchymal cells may contribute to drug transport and metabolism.\n\nOverall, the data we provide constitute a unique resource for exploring the proteomes of the major types of human liver cells, which will facilitate an improved understanding of the human liver in health and disease.", "doi": "10.1111/liv.14452", "pmid": "32243721", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T12:20:30.551Z", "modified": "2026-08-21T12:20:30.575Z"}, {"entity": "publication", "iuid": "1d9e276d0f074c81b49252208fbe8ace", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e276d0f074c81b49252208fbe8ace.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/1d9e276d0f074c81b49252208fbe8ace"}}, "title": "Image-Based Quantification of Cell Debris as a Measure of Apoptosis.", "authors": [{"family": "\u00d6lander", "given": "Magnus", "initials": "M", "orcid": "0000-0002-4502-8184", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/dacf28f90f084718817f085fed119acb.json"}}, {"family": "Handin", "given": "Niklas", "initials": "N"}, {"family": "Artursson", "given": "Per", "initials": "P"}], "type": "journal article", "published": "2019-05-07", "journal": {"title": "Anal. Chem.", "issn": "1520-6882", "volume": "91", "issue": "9", "pages": "5548-5552", "issn-l": "0003-2700"}, "abstract": "Apoptosis is a controlled form of cell death that can be induced by various diseases and exogenous toxicants. Common apoptosis-detection methods rely on fluorescent markers, which necessitate the use of costly reagents and time-consuming labeling procedures. Label-free methods avoid these problems, but often require specialized instruments instead. Here, we utilize apoptotic-cell disintegration to develop a novel label-free detection method based on the quantification of subcellular debris particles in bright-field-microscopy images. Debris counts show strong correlations with fluorescence-based annexin V staining and can be used to study concentration-dependent and temporal apoptosis activation. The method is rapid, low-cost, and easy to apply, as the only experimental step comprises bright-field imaging of culture-media samples followed by automated image processing. The late-stage nature of the debris measurement means that the method can complement other, established apoptosis assays, and its accessibility will allow a wider community of researchers to study apoptotic cell death.", "doi": "10.1021/acs.analchem.9b01243", "pmid": "31001971", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-21T11:32:40.187Z", "modified": "2026-08-21T11:32:40.247Z"}]}