{"entity": "researcher", "timestamp": "2026-09-28T11:20:20.742Z", "family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "affiliations": ["Krefting Research Centre, Institute of Medicine, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/d878ea7958b04195b1a422ac4a1493de.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/d878ea7958b04195b1a422ac4a1493de"}}, "publications": [{"entity": "publication", "iuid": "6bc51e3223de45e39bd150c6e106351b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6bc51e3223de45e39bd150c6e106351b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6bc51e3223de45e39bd150c6e106351b"}}, "title": "Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells.", "authors": [{"family": "Yin", "given": "Yanan", "initials": "Y", "orcid": "0000-0002-9894-1938", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8779ea1ddfaf4d6fa2b892e043f635fc.json"}}, {"family": "Shelke", "given": "Ganesh Vilas", "initials": "GV", "orcid": "0000-0001-5883-8082", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1f3f7b6c1bf46ddba8d21c22ff2f5ef.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/360a1db0f6794235ac1466456cace77b.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d878ea7958b04195b1a422ac4a1493de.json"}}], "type": "journal article", "published": "2020-05-01", "journal": {"title": "Respir. Res.", "issn": "1465-993X", "volume": "21", "issue": "1", "pages": "101", "issn-l": "1465-9921"}, "abstract": "In the airways, mast cells are present in close vicinity to epithelial cells, and they can interact with each other via multiple factors, including extracellular vesicles (EVs). Mast cell-derived EVs have a large repertoire of cargos, including proteins and RNA, as well as surface DNA. In this study, we hypothesized that these EVs can induce epithelial to mesenchymal transition (EMT) in airway epithelial cells.\n\nIn this in-vitro study we systematically determined the effects of mast cell-derived EVs on epithelial A549 cells. We determined the changes that are induced by EVs on A549 cells at both the RNA and protein levels. Moreover, we also analyzed the rapid changes in phosphorylation events in EV-recipient A549 cells using a phosphorylated protein microarray. Some of the phosphorylation-associated events associated with EMT were validated using immunoblotting.\n\nMorphological and transcript analysis of epithelial A549 cells indicated that an EMT-like phenotype was induced by the EVs. Transcript analysis indicated the upregulation of genes involved in EMT, including TWIST1, MMP9, TGFB1, and BMP-7. This was accompanied by downregulation of proteins such as E-cadherin and upregulation of Slug-Snail and matrix metalloproteinases. Additionally, our phosphorylated-protein microarray analysis revealed proteins associated with the EMT cascade that were upregulated after EV treatment. We also found that transforming growth factor beta-1, a well-known EMT inducer, is associated with EVs and mediates the EMT cascade induced in the A549 cells.\n\nMast cell-derived EVs mediate the induction of EMT in epithelial cells, and our evidence suggests that this is triggered through the induction of protein phosphorylation cascades.", "doi": "10.1186/s12931-020-01346-8", "pmid": "32357878", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7193353"}, {"db": "pii", "key": "10.1186/s12931-020-01346-8"}], "notes": [], "created": "2026-09-23T08:42:23.412Z", "modified": "2026-09-23T14:52:35.296Z"}, {"entity": "publication", "iuid": "e5b453ec009d491bb9db23b6a62a656e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e5b453ec009d491bb9db23b6a62a656e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e5b453ec009d491bb9db23b6a62a656e"}}, "title": "Endosomal signalling via exosome surface TGF\u03b2-1.", "authors": [{"family": "Shelke", "given": "Ganesh Vilas", "initials": "GV", "orcid": "0000-0001-5883-8082", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1f3f7b6c1bf46ddba8d21c22ff2f5ef.json"}}, {"family": "Yin", "given": "Yanan", "initials": "Y", "orcid": "0000-0002-9894-1938", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8779ea1ddfaf4d6fa2b892e043f635fc.json"}}, {"family": "Jang", "given": "Su Chul", "initials": "SC", "orcid": "0000-0003-3326-1007", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1d52ae24b4e24b668b6e609ebae5130b.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/360a1db0f6794235ac1466456cace77b.json"}}, {"family": "Wennmalm", "given": "Stefan", "initials": "S"}, {"family": "Hoffmann", "given": "Hans J\u00fcrgen", "initials": "HJ"}, {"family": "Li", "given": "Li", "initials": "L"}, {"family": "Gho", "given": "Yong Song", "initials": "YS"}, {"family": "Nilsson", "given": "Jonas Andreas", "initials": "JA", "orcid": "0000-0003-0346-6837", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0e2de404cd3e46e7b99bae1f0a5c4e61.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d878ea7958b04195b1a422ac4a1493de.json"}}], "type": "journal article", "published": "2019-09-20", "journal": {"title": "J Extracell Vesicles", "issn": "2001-3078", "volume": "8", "issue": "1", "pages": "1650458", "issn-l": null}, "abstract": "Extracellular vesicles such as exosomes convey biological messages between cells, either by surface-to-surface interaction or by shuttling of bioactive molecules to a recipient cell's cytoplasm. Here we show that exosomes released by mast cells harbour both active and latent transforming growth factor \u03b2-1 (TGF\u03b2-1) on their surfaces. The latent form of TGF\u03b2-1 is associated with the exosomes via heparinase-II and pH-sensitive elements. These vesicles traffic to the endocytic compartment of recipient human mesenchymal stem cells (MSCs) within 60 min of exposure. Further, the exosomes-associated TGF\u03b2-1 is retained within the endosomal compartments at the time of signalling, which results in prolonged cellular signalling compared to free-TGF\u03b2-1. These exosomes induce a migratory phenotype in primary MSCs involving SMAD-dependent pathways. Our results show that mast cell-derived exosomes are decorated with latent TGF\u03b2-1 and are retained in recipient MSC endosomes, influencing recipient cell migratory phenotype. We conclude that exosomes can convey signalling within endosomes by delivering bioactive surface ligands to this intracellular compartment.", "doi": "10.1080/20013078.2019.1650458", "pmid": "31595182", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6764367"}, {"db": "pii", "key": "1650458"}], "notes": [], "created": "2026-09-23T06:47:22.392Z", "modified": "2026-09-23T06:47:22.703Z"}]}