{"entity": "researcher", "timestamp": "2026-08-20T20:50:36.991Z", "family": "Kemp", "given": "Grant", "initials": "G", "orcid": "0000-0001-5125-2740", "affiliations": ["Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4"}}, "publications": [{"entity": "publication", "iuid": "71f0280c5fa44aff85961bc6c771cbd5", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/71f0280c5fa44aff85961bc6c771cbd5.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/71f0280c5fa44aff85961bc6c771cbd5"}}, "title": "Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli.", "authors": [{"family": "Elfageih", "given": "Rageia", "initials": "R", "orcid": "0000-0003-0506-1470", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a76db32fb3f44bd992423d22ebef0293.json"}}, {"family": "Karyolaimos", "given": "Alexandros", "initials": "A", "orcid": "0000-0003-2264-3958", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4d43d202f8684c09a3e34ca0d26cabc4.json"}}, {"family": "Kemp", "given": "Grant", "initials": "G", "orcid": "0000-0001-5125-2740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4.json"}}, {"family": "de Gier", "given": "Jan-Willem", "initials": "JW", "orcid": "0000-0001-5537-4358", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5868d65a24344c5da61ca3cacd29a558.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}, {"family": "Kudva", "given": "Renuka", "initials": "R", "orcid": "0000-0003-0426-3716", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a8f164c44cca4540965f66c2829f624e.json"}}], "type": "journal article", "published": "2020-10-00", "journal": {"title": "Protein Sci.", "issn": "1469-896X", "volume": "29", "issue": "10", "pages": "2028-2037", "issn-l": "0961-8368"}, "abstract": "Cotranslational protein folding studies using Force Profile Analysis, a method where the SecM translational arrest peptide is used to detect folding-induced forces acting on the nascent polypeptide, have so far been limited mainly to small domains of cytosolic proteins that fold in close proximity to the translating ribosome. In this study, we investigate the cotranslational folding of the periplasmic, disulfide bond-containing Escherichia coli protein alkaline phosphatase (PhoA) in a wild-type strain background and a strain background devoid of the periplasmic thiol: disulfide interchange protein DsbA. We find that folding-induced forces can be transmitted via the nascent chain from the periplasm to the polypeptide transferase center in the ribosome, a distance of ~160 \u00c5, and that PhoA appears to fold cotranslationally via at least two disulfide-stabilized folding intermediates. Thus, Force Profile Analysis can be used to study cotranslational folding of proteins in an extra-cytosolic compartment, like the periplasm.", "doi": "10.1002/pro.3927", "pmid": "32790204", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7513700"}], "notes": [], "created": "2026-08-20T06:35:05.418Z", "modified": "2026-08-20T06:35:05.625Z"}, {"entity": "publication", "iuid": "4a7cb3934b1f44359966f38a422fae00", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4a7cb3934b1f44359966f38a422fae00.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4a7cb3934b1f44359966f38a422fae00"}}, "title": "Cotranslational folding of alkaline phosphatase in the periplasm of Escherichia coli", "authors": [{"family": "Elfageih", "given": "Rageia", "initials": "R"}, {"family": "Karyolaimos", "given": "Alexandros", "initials": "A", "orcid": "0000-0003-2264-3958", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4d43d202f8684c09a3e34ca0d26cabc4.json"}}, {"family": "Kemp", "given": "Grant", "initials": "G", "orcid": "0000-0001-5125-2740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4.json"}}, {"family": "de Gier", "given": "Jan Willem", "initials": "JW", "orcid": "0000-0001-5537-4358", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5868d65a24344c5da61ca3cacd29a558.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}, {"family": "Kudva", "given": "Renuka", "initials": "R", "orcid": "0000-0003-0426-3716", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a8f164c44cca4540965f66c2829f624e.json"}}], "type": "posted-content", "published": "2020-07-06", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2020.07.06.189464", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:58:02.348Z", "modified": "2026-08-20T09:58:02.461Z"}, {"entity": "publication", "iuid": "529bcd0b6fc44702ac6b78b88503b316", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/529bcd0b6fc44702ac6b78b88503b316.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/529bcd0b6fc44702ac6b78b88503b316"}}, "title": "Cotranslational folding cooperativity of contiguous domains of \u03b1-spectrin.", "authors": [{"family": "Kemp", "given": "Grant", "initials": "G", "orcid": "0000-0001-5125-2740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4.json"}}, {"family": "Nilsson", "given": "Ola B", "initials": "OB"}, {"family": "Tian", "given": "Pengfei", "initials": "P", "orcid": "0000-0003-0255-8692", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2c8345e3930e4a52b396837a1c099e3f.json"}}, {"family": "Best", "given": "Robert B", "initials": "RB", "orcid": "0000-0002-7893-3543", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/36c5cc15e14e412fb8798666bd48f00f.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "journal article", "published": "2020-06-23", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "117", "issue": "25", "pages": "14119-14126", "issn-l": "0027-8424"}, "abstract": "Proteins synthesized in the cell can begin to fold during translation before the entire polypeptide has been produced, which may be particularly relevant to the folding of multidomain proteins. Here, we study the cotranslational folding of adjacent domains from the cytoskeletal protein \u03b1-spectrin using force profile analysis (FPA). Specifically, we investigate how the cotranslational folding behavior of the R15 and R16 domains are affected by their neighboring R14 and R16, and R15 and R17 domains, respectively. Our results show that the domains impact each other's folding in distinct ways that may be important for the efficient assembly of \u03b1-spectrin, and may reduce its dependence on chaperones. Furthermore, we directly relate the experimentally observed yield of full-length protein in the FPA assay to the force exerted by the folding protein in piconewtons. By combining pulse-chase experiments to measure the rate at which the arrested protein is converted into full-length protein with a Bell model of force-induced rupture, we estimate that the R16 domain exerts a maximal force on the nascent chain of \u223c15 pN during cotranslational folding.", "doi": "10.1073/pnas.1909683117", "pmid": "32513720", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7322005"}, {"db": "pii", "key": "1909683117"}], "notes": [], "created": "2026-08-20T09:30:31.613Z", "modified": "2026-08-20T09:30:31.734Z"}, {"entity": "publication", "iuid": "5ad7d6b43851434ab564834089559d4b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5ad7d6b43851434ab564834089559d4b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5ad7d6b43851434ab564834089559d4b"}}, "title": "Cotranslational folding cooperativity of contiguous domains of \u03b1-spectrin", "authors": [{"family": "Kemp", "given": "Grant", "initials": "G", "orcid": "0000-0001-5125-2740", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d715b8af2972418aa144b2ca11487ce4.json"}}, {"family": "Nilsson", "given": "Ola B", "initials": "OB"}, {"family": "Tian", "given": "Pengfei", "initials": "P"}, {"family": "Best", "given": "Robert B", "initials": "RB", "orcid": "0000-0002-7893-3543", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/36c5cc15e14e412fb8798666bd48f00f.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "posted-content", "published": "2019-05-29", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/653360", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:13:51.259Z", "modified": "2026-08-20T11:13:51.326Z"}]}