{"entity": "researcher", "timestamp": "2026-08-20T20:57:11.798Z", "family": "Sandalova", "given": "Tatiana", "initials": "T", "orcid": "0000-0002-7694-6420", "affiliations": ["Science for Life Laboratory, Department of Medicine Solna, Karolinska Institute, & Division of Infectious Diseases, Karolinska University Hospital, SE-171 77, Solna, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2ea3d2ec9e94fe3876f88bd0786c24b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2ea3d2ec9e94fe3876f88bd0786c24b"}}, "publications": [{"entity": "publication", "iuid": "2108b46955d444a39235fed52f4243dc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2108b46955d444a39235fed52f4243dc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2108b46955d444a39235fed52f4243dc"}}, "title": "Mutational insights into human kynurenine aminotransferase 1: modulation of transamination and \u03b2-elimination activities across diverse substrates.", "authors": [{"family": "Selvam", "given": "Arun Kumar", "initials": "AK", "orcid": "0000-0002-9854-2048", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8e7a8d1d439a4ed0aebad8389a7253e2.json"}}, {"family": "Sun", "given": "Renhua", "initials": "R", "orcid": "0000-0002-8203-4946", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/29b44e08db3a4482a6a40bbda52fe815.json"}}, {"family": "Razaghi", "given": "Ali", "initials": "A", "orcid": "0000-0001-8771-998X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b978f17f56764dfb8fbef3fd06d10214.json"}}, {"family": "Salter", "given": "Hugh", "initials": "H", "orcid": "0000-0001-5705-2950", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c405a1c865d34a769095ada5edc8d04b.json"}}, {"family": "Sandalova", "given": "Tatiana", "initials": "T", "orcid": "0000-0002-7694-6420", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2ea3d2ec9e94fe3876f88bd0786c24b.json"}}, {"family": "Bj\u00f6rnstedt", "given": "Mikael", "initials": "M", "orcid": "0000-0003-2831-3837", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/08e79d991fab47c18b7390c7ddc12963.json"}}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}], "type": "journal article", "published": "2025-08-18", "journal": {"title": "Biochem. J.", "issn": "1470-8728", "volume": "482", "issue": "16", "pages": "1163-1180", "issn-l": "0264-6021"}, "abstract": "Human kynurenine aminotransferase 1 (hKYAT1) plays a crucial role in the transamination of aromatic amino acids and kynurenine. This promiscuous homodimeric enzyme transaminates various amino acids into their corresponding \u03b1-keto acids. Additionally, hKYAT1 is known to catalyze the \u03b2-elimination of cysteine-S conjugates and cysteine-Se conjugates. In this study, we performed mutational analyses of hKYAT1, targeting its catalytic, ligand-binding, and substrate-binding sites. The transamination activity of 13 mutant variants was systematically evaluated against sixteen different amino acid substrates, including kynurenine, selenomethionine (SeMet), and Se-methylselenocysteine (MSC), as well as for the \u03b2-elimination of SeMet and MSC. Our results demonstrate that mutations of residues E27 in the catalytic site and H279 in the substratestabilizing site significantly enhanced the transamination of several amino acids, including phenylalanine, tryptophan, histidine, and MSC. The H279F mutation increased transamination and \u03b2-elimination of MSC by 2- and 1.5-fold, respectively. Furthermore, mutation at the ligand-binding residues R398, F125, and N185 substantially reduced MSC transamination activity of hKYAT1. Interestingly, none of the tested mutations affected the transamination of l-kynurenine, a natural substrate of hKYAT1. Altogether, these findings support future investigation into hKYAT1 as a modifiable target in selenium-mediated anticancer approaches.", "doi": "10.1042/BCJ20253178", "pmid": "40785633", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12493182"}, {"db": "pii", "key": "236366"}], "notes": [], "created": "2026-08-20T09:28:22.125Z", "modified": "2026-08-20T09:28:22.422Z"}, {"entity": "publication", "iuid": "0102d2378dc1467b93cf164440fd96fc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0102d2378dc1467b93cf164440fd96fc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0102d2378dc1467b93cf164440fd96fc"}}, "title": "Crystallographic and NMR studies of Streptococcus pneumonia LCP protein Psr Sp indicate the importance of dynamics in four long loops for ligand specificity", "authors": [{"family": "Sandalova", "given": "Tatyana", "initials": "T", "orcid": "0000-0002-7694-6420", "researcher": {"href": 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"given": "Birgitta", "initials": "B", "orcid": "0000-0002-5429-4759", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/40da20f498284552b13443f9109b7e32.json"}}, {"family": "Agback", "given": "Tatiana", "initials": "T", "orcid": "0000-0003-1325-6024", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/45cf38c8b15946cda75b81cde94bbdfe.json"}}, {"family": "Lesovoy", "given": "Dmitry", "initials": "D", "orcid": "0000-0002-9130-715X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/706f3a39328e4e17823901ccc52b27c1.json"}}, {"family": "Agback", "given": "Peter", "initials": "P", "orcid": "0000-0003-2226-0746", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/eae6fd83468f4ddfbbb7609d710dec6c.json"}}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}], "type": "posted-content", "published": "2024-10-21", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.10.21.619401", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:58:53.965Z", "modified": "2026-08-20T10:58:54.223Z"}, {"entity": "publication", "iuid": "8979c7df59254dd9822b926c7a0cb26a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/8979c7df59254dd9822b926c7a0cb26a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/8979c7df59254dd9822b926c7a0cb26a"}}, "title": "Towards understanding of allostery in MALT1: a possible role of interdomain motions as revealed by NMR and AlphaFold", "authors": [{"family": "Wallerstein", "given": "Johan", "initials": "J", "orcid": "0009-0005-9832-5210", "researcher": {"href": 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"https://publications-affiliated.scilifelab.se/researcher/c237ffc07068465286d7b903683ed3ab.json"}}, {"family": "Achour", "given": "Adnane", "initials": "A", "orcid": "0000-0003-0432-710X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/65fba324156a4fcbafbe636e642587a9.json"}}, {"family": "Agback", "given": "Tatiana", "initials": "T", "orcid": "0000-0003-1325-6024", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/45cf38c8b15946cda75b81cde94bbdfe.json"}}, {"family": "Orekhov", "given": "Vladislav", "initials": "V", "orcid": "0000-0002-7892-6896", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a2bf27272cbc40e7b01f24b4324f0fb2.json"}}], "type": "posted-content", "published": "2024-02-01", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.02.01.578365", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:51:57.455Z", "modified": "2026-08-20T10:51:57.825Z"}, {"entity": "publication", "iuid": "49529f6dfbd942869ba0f99c6123c7a9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/49529f6dfbd942869ba0f99c6123c7a9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/49529f6dfbd942869ba0f99c6123c7a9"}}, "title": "Novel conformational filter allows unambiguous identification of specific conformational ensembles for the large NS3pro/NS2B Dengue-associated protease; Implications for the appropriate choice of protein conformations in drug discovery", "authors": [{"family": "Agback", "given": "Tatiana", "initials": "T"}, {"family": "Lesovoy", "given": "Dmitry", "initials": "D"}, {"family": "Han", "given": "Xiao", "initials": "X"}, {"family": "Lomzov", "given": "Alexander", "initials": "A"}, {"family": "Sun", "given": "Renhua", "initials": "R", "orcid": "0000-0002-8203-4946", "researcher": {"href": 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"https://publications-affiliated.scilifelab.se/publication/a4a5c1bd18fa4fde8ae8b3be118e6710"}}, "title": "PD-1 expression on NK cells can be related to cytokine stimulation and tissue residency", "authors": [{"family": "Wagner", "given": "Arnika K", "initials": "AK", "orcid": "0000-0002-0339-8259", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c11bf281adcd401c8a11244b59f34506.json"}}, {"family": "Kadri", "given": "Nadir", "initials": "N"}, {"family": "Tibbitt", "given": "Chris", "initials": "C"}, {"family": "van de Ven", "given": "Koen", "initials": "K"}, {"family": "Bagawath-Singh", "given": "Sunitha", "initials": "S", "orcid": "0000-0002-2812-545X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2dbc874ed9724712afd3abc601bb2b71.json"}}, {"family": "Oliinyk", "given": "Denys", "initials": "D", "orcid": "0000-0002-2814-460X", "researcher": {"href": 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