{"entity": "researcher", "timestamp": "2026-08-20T20:40:54.661Z", "family": "Metola", "given": "Ane", "initials": "A", "orcid": "0000-0002-2885-7634", "affiliations": ["Department of Biochemistry and Biophysics, Stockholm University, Stockholm SE-106 91, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7"}}, "publications": [{"entity": "publication", "iuid": "36b68ca5b04e4d5ca613883e68f05b76", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/36b68ca5b04e4d5ca613883e68f05b76.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/36b68ca5b04e4d5ca613883e68f05b76"}}, "title": "Cotranslational membrane insertion of the voltage-sensitive K+ channel KvAP.", "authors": [{"family": "Westerfield", "given": "Justin M", "initials": "JM"}, {"family": "Kozojedov\u00e1", "given": "Petra", "initials": "P", "orcid": "0009-0005-0015-6050", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/12843a92cb9344d1b5a19a0d6e6f96aa.json"}}, {"family": "Juli", "given": "Cara", "initials": "C"}, {"family": "Metola", "given": "Ane", "initials": "A", "orcid": "0000-0002-2885-7634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "journal article", "published": "2025-04-08", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "122", "issue": "14", "pages": "e2412492122", "issn-l": "0027-8424"}, "abstract": "Voltage-sensor domains (VSDs), found in many voltage-sensitive ion channels and enzymes, are composed of four transmembrane helices (TMHs), including the atypical, highly positively charged S4 helix. VSDs are cotranslationally inserted into the membrane, raising the question of how the highly charged S4 helix is integrated into the lipid bilayer as it exits the ribosome. Here, we have used force profile analysis (FPA) to follow the cotranslational insertion of the six-TMH KvAP voltage-sensitive ion channel into the Escherichia coli inner membrane. We find that the insertion process proceeds through three semi-independent steps: i) insertion of the S1-S2 helix hairpin, ii) insertion of the S3-S5 helices, and iii) insertion of the Pore and S6 helices. Our analysis highlights the importance of the concerted insertion of helical hairpins, the dramatic influence of the positively charged residues in S4, and the unexpectedly strong forces and effects on downstream TMHs elicited by amphipathic and re-entrant helices.", "doi": "10.1073/pnas.2412492122", "pmid": "40163725", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12002286"}], "notes": [], "created": "2026-08-20T09:31:34.716Z", "modified": "2026-08-20T09:31:34.872Z"}, {"entity": "publication", "iuid": "7cc03b338f0e4d23873d077a78419126", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7cc03b338f0e4d23873d077a78419126.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7cc03b338f0e4d23873d077a78419126"}}, "title": "Cotranslational membrane insertion of the voltage-sensitive K + channel KvAP", "authors": [{"family": "Westerfield", "given": "Justin M", "initials": "JM", "orcid": "0000-0002-3937-5833", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/abd6b68fba914d529ef0ad62466a4ebc.json"}}, {"family": "Kozojedov\u00e1", "given": "Petra", "initials": "P"}, {"family": "Juli", "given": "Cara", "initials": "C"}, {"family": "Metola", "given": "Ane", "initials": "A", "orcid": "0000-0002-2885-7634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "posted-content", "published": "2024-05-29", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.05.28.596144", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:54:57.840Z", "modified": "2026-08-20T10:54:57.924Z"}, {"entity": "publication", "iuid": "10051fb20f9a490c8d7fafa4d0b1886a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/10051fb20f9a490c8d7fafa4d0b1886a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/10051fb20f9a490c8d7fafa4d0b1886a"}}, "title": "Residue-by-residue analysis of cotranslational membrane protein integration in vivo.", "authors": [{"family": "Nicolaus", "given": "Felix", "initials": "F", "orcid": "0000-0001-9230-8544", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9f47078a41e242c98a1b2a3c2ad0ebe9.json"}}, {"family": "Metola", "given": "Ane", "initials": "A", "orcid": "0000-0002-2885-7634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7.json"}}, {"family": "Mermans", "given": "Daphne", "initials": "D", "orcid": "0000-0001-6001-5608", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27a2db6738b94dd188eff74e4705bf37.json"}}, {"family": "Liljenstr\u00f6m", "given": "Amanda", "initials": "A"}, {"family": "Kr\u010d", "given": "Ajda", "initials": "A"}, {"family": "Abdullahi", "given": "Salmo Mohammed", "initials": "SM"}, {"family": "Zimmer", "given": "Matthew", "initials": "M", "orcid": "0000-0002-1437-2636", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab4c6e06a9a84cf9b710748f1ad659bf.json"}}, {"family": "Miller Iii", "given": "Thomas F", "initials": "TF", "orcid": "0000-0002-1882-5380", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d29bc3f81346afa7ca8b713df24a86.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "journal article", "published": "2021-02-08", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "10", "issn-l": "2050-084X"}, "abstract": "We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected complexities in the membrane integration process. We find that an N-terminal cytoplasmic domain can fold in the ribosome exit tunnel before membrane integration starts, that charged residues and membrane-interacting segments such as re-entrant loops and surface helices flanking a transmembrane helix (TMH) can advance or delay membrane integration, and that point mutations in an upstream TMH can affect the pulling forces generated by downstream TMHs in a highly position-dependent manner, suggestive of residue-specific interactions between TMHs during the integration process. Our results support the 'sliding' model of translocon-mediated membrane protein integration, in which hydrophobic segments are continually exposed to the lipid bilayer during their passage through the SecYEG translocon.", "doi": "10.7554/eLife.64302", "pmid": "33554862", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7886326"}, {"db": "pii", "key": "64302"}], "notes": [], "created": "2026-08-20T13:53:20.604Z", "modified": "2026-08-20T13:53:20.732Z"}, {"entity": "publication", "iuid": "df2c8bb10e134c1bbdb453843cfa9793", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/df2c8bb10e134c1bbdb453843cfa9793.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/df2c8bb10e134c1bbdb453843cfa9793"}}, "title": "Author response: Residue-by-residue analysis of cotranslational membrane protein integration in vivo", "authors": [{"family": "Nicolaus", "given": "Felix", "initials": "F", "orcid": "0000-0001-9230-8544", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9f47078a41e242c98a1b2a3c2ad0ebe9.json"}}, {"family": "Metola", "given": "Ane", "initials": "A", "orcid": "0000-0002-2885-7634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d15b6e5328914e6d8de881bd6a78a1a7.json"}}, {"family": "Mermans", "given": "Daphne", "initials": "D", "orcid": "0000-0001-6001-5608", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27a2db6738b94dd188eff74e4705bf37.json"}}, {"family": "Liljenstr\u00f6m", "given": "Amanda", "initials": "A"}, {"family": "Kr\u010d", "given": "Ajda", "initials": "A"}, {"family": "Abdullahi", "given": "Salmo Mohammed", "initials": "SM"}, {"family": "Zimmer", "given": "Matthew", "initials": "M", "orcid": "0000-0002-1437-2636", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ab4c6e06a9a84cf9b710748f1ad659bf.json"}}, {"family": "Miller III", "given": "Thomas F", "initials": "TF", "orcid": "0000-0002-1882-5380", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/27d29bc3f81346afa7ca8b713df24a86.json"}}, {"family": "von Heijne", "given": "Gunnar", "initials": "G", "orcid": "0000-0002-4490-8569", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/529a460e668a479ca7d9b9271375ef9f.json"}}], "type": "peer-review", "published": "2021-02-01", "journal": {"issn-l": null}, "abstract": null, "doi": "10.7554/elife.64302.sa2", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:53:22.637Z", "modified": "2026-08-20T13:53:22.681Z"}]}