{"entity": "researcher", "timestamp": "2026-08-20T21:09:10.904Z", "family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "affiliations": ["Division of Immunology and Allergy, Department of Medicine Solna, Karolinska Institute and University Hospital, Stockholm, Sweden. eduardo.villablanca@ki.se.", "Center of Molecular Medicine, Stockholm, Sweden. eduardo.villablanca@ki.se."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0"}}, "publications": [{"entity": "publication", "iuid": "72a59460db0b4dbeb14e1dd17cc8da39", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/72a59460db0b4dbeb14e1dd17cc8da39.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/72a59460db0b4dbeb14e1dd17cc8da39"}}, "title": "A versatile tissue-rolling technique for spatial-omics analyses of the entire murine gastrointestinal tract.", "authors": [{"family": "Monasterio", "given": "Gustavo", "initials": "G", "orcid": "0000-0001-6336-132X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ce06c970b0f84b749499513116913eb6.json"}}, {"family": "Morales", "given": "Rodrigo A", "initials": "RA", "orcid": "0000-0003-4382-5777", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/32b43121030e4531b11e3b04938e765e.json"}}, {"family": "Bejarano", "given": "David A", "initials": "DA", "orcid": "0000-0001-7804-0131", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/78885dd0abe24535993f60bd2e6b9829.json"}}, {"family": "Abalo", "given": "Xes\u00fas M", "initials": "XM", "orcid": "0000-0002-1643-0705", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ccb96c37f02b45d290b89f5b615052df.json"}}, {"family": "Fransson", "given": "Jennifer", "initials": "J"}, {"family": "Larsson", "given": "Ludvig", "initials": "L"}, {"family": "Schlitzer", "given": "Andreas", "initials": "A", "orcid": "0000-0001-7662-3712", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79413d89067d43b292f2ca4b8a4d66f1.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J", "orcid": "0000-0003-4313-1601", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d9fa47767cd14ef2b9528c8b998cf095.json"}}, {"family": "Das", "given": "Srustidhar", "initials": "S"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}], "type": "journal article", "published": "2024-10-00", "journal": {"title": "Nat Protoc", "issn": "1750-2799", "volume": "19", "issue": "10", "pages": "3085-3137", "issn-l": null}, "abstract": "Tissues are dynamic and complex biological systems composed of specialized cell types that interact with each other for proper biological function. To comprehensively characterize and understand the cell circuitry underlying biological processes within tissues, it is crucial to preserve their spatial information. Here we report a simple mounting technique to maximize the area of the tissue to be analyzed, encompassing the whole length of the murine gastrointestinal (GI) tract, from mouth to rectum. Using this method, analysis of the whole murine GI tract can be performed in a single slide not only by means of histological staining, immunohistochemistry and in situ hybridization but also by multiplexed antibody staining and spatial transcriptomic approaches. We demonstrate the utility of our method in generating a comprehensive gene and protein expression profile of the whole GI tract by combining the versatile tissue-rolling technique with a cutting-edge transcriptomics method (Visium) and two cutting-edge proteomics methods (ChipCytometry and CODEX-PhenoCycler) in a systematic and easy-to-follow step-by-step procedure. The entire process, including tissue rolling, processing and sectioning, can be achieved within 2-3 d for all three methods. For Visium spatial transcriptomics, an additional 2 d are needed, whereas for spatial proteomics assays (ChipCytometry and CODEX-PhenoCycler), another 3-4 d might be considered. The whole process can be accomplished by researchers with skills in performing murine surgery, and standard histological and molecular biology methods.", "doi": "10.1038/s41596-024-01001-2", "pmid": "38906985", "labels": [], "xrefs": [{"db": "pii", "key": "10.1038/s41596-024-01001-2"}, {"db": "GEO", "key": "GSE169749"}], "notes": [], "created": "2026-08-20T09:04:01.223Z", "modified": "2026-08-20T09:04:01.475Z"}, {"entity": "publication", "iuid": "709815f29bb444d3b23a77ef17185bcf", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/709815f29bb444d3b23a77ef17185bcf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/709815f29bb444d3b23a77ef17185bcf"}}, "title": "The spatial transcriptomic landscape of the healing intestine following damage", "authors": [{"family": "Parigi", "given": "Sara M", "initials": "SM"}, {"family": "Larsson", "given": "Ludvig", "initials": "L"}, {"family": "Das", "given": "Srustidhar", "initials": "S"}, {"family": "Ramirez Flores", "given": "Ricardo O", "initials": "RO", "orcid": "0000-0003-0087-371X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da240d88f24d4c6fbdec6c06c1136e74.json"}}, {"family": "Frede", "given": "Annika", "initials": "A"}, {"family": "Tripathi", "given": "Kumar P", "initials": "KP"}, {"family": "Diaz", "given": "Oscar E", "initials": "OE"}, {"family": "Selin", "given": "Katja", "initials": "K"}, {"family": "Morales", "given": "Rodrigo A", "initials": "RA"}, {"family": "Luo", "given": "Xinxin", "initials": "X"}, {"family": "Monasterio", "given": "Gustavo", "initials": "G"}, {"family": "Engblom", "given": "Camilla", "initials": "C"}, {"family": "Gagliani", "given": "Nicola", "initials": "N"}, {"family": "Saez-Rodriguez", "given": "Julio", "initials": "J", "orcid": "0000-0002-8552-8976", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/504df2e61c124714b9afecf767798a00.json"}}, {"family": "Lundeberg", "given": "Joakim", "initials": "J"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}], "type": "posted-content", "published": "2021-07-03", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2021.07.01.450768", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:05:31.070Z", "modified": "2026-08-20T10:05:31.176Z"}, {"entity": "publication", "iuid": "87b5d0b9ef94406ea6311bd755326d5f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/87b5d0b9ef94406ea6311bd755326d5f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/87b5d0b9ef94406ea6311bd755326d5f"}}, "title": "B Cell Expansion Hinders the Stroma-Epithelium Regenerative Crosstalk During Mucosal Healing", "authors": [{"family": "Frede", "given": "Annika", "initials": "A"}, {"family": "Tripathi", "given": "Kumar Parijat", "initials": "KP", "orcid": "0000-0002-3998-599X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/949d543dbae548cbb5c0310a5f235964.json"}}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Monasterio", "given": "Gustavo", "initials": "G", "orcid": "0000-0001-6336-132X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ce06c970b0f84b749499513116913eb6.json"}}, {"family": "Ramirez Flores", "given": "Ricardo O", "initials": "RO"}, {"family": "Sorini", "given": "Chiara", "initials": "C", "orcid": "0000-0002-6803-8377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/652b92b02a9448179fb0bf7648ed30b9.json"}}, {"family": "larsson", "given": "ludvig", "initials": "l"}, {"family": "Luo", "given": "Xinxin", "initials": "X"}, {"family": "Novella-Rausell", "given": "Claudio", "initials": "C", "orcid": "0000-0002-7383-6090", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0ce5631e59ac468cb9e5d4e8ab16d34e.json"}}, {"family": "Zagami", "given": "Chiara", "initials": "C", "orcid": "0000-0002-5246-3432", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/18e9f7dd5e0448c49dfe7a3eeaba2b84.json"}}, {"family": "Hu", "given": "Yue O O", "initials": "YOO"}, {"family": "Engblom", "given": "Camilla", "initials": "C", "orcid": "0000-0001-5090-4161", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7e39a73daed74f38be122ae9c564810c.json"}}, {"family": "Mittenzwei", "given": "Romy", "initials": "R"}, {"family": "H\u00f6velmeyer", "given": "Nadine", "initials": "N"}, {"family": "Lundeberg", "given": "Joakim", "initials": "J"}, {"family": "Das", "given": "Srustidhar", "initials": "S"}, {"family": "Saez-Rodriguez", "given": "Julio", "initials": "J"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}], "type": "posted-content", "published": "2021-00-00", "journal": {"issn-l": null}, "abstract": null, "doi": "10.2139/ssrn.3945928", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:01:13.227Z", "modified": "2026-08-20T13:01:13.428Z"}, {"entity": "publication", "iuid": "bd4ebc826fe6497e8d49869cf0bccace", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bd4ebc826fe6497e8d49869cf0bccace.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bd4ebc826fe6497e8d49869cf0bccace"}}, "title": "Liver X Receptor regulates Th17 and ROR\u03b3t + Treg cells by distinct mechanisms", "authors": [{"family": "Parigi", "given": "Sara M", "initials": "SM"}, {"family": "Das", "given": "Srustidhar", "initials": "S", "orcid": "0000-0002-0833-588X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d2ec52fedd6e4cda931ed56b138f6d55.json"}}, {"family": "Frede", "given": "Annika", "initials": "A"}, {"family": "Cardoso", "given": "Rebeca F", "initials": "RF", "orcid": "0000-0002-0497-8909", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d959ca5007d64d5388703d62a18474a0.json"}}, {"family": "Tripathi", "given": "Kumar Parijat", "initials": "KP"}, {"family": "Do\u00f1as", "given": "Cristian", "initials": "C"}, {"family": "Hu", "given": "Yue O O", "initials": "YOO"}, {"family": "Antonson", "given": "Per", "initials": "P"}, {"family": "Engstrand", "given": "Lars", "initials": "L"}, {"family": "Gustafsson", "given": "Jan \u00c5ke", "initials": "J\u00c5"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}], "type": "posted-content", "published": "2019-10-25", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/818369", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:15:06.059Z", "modified": "2026-08-20T11:15:06.181Z"}, {"entity": "publication", "iuid": "d001de3cfeec44d9b818769dbbf3e9fa", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d001de3cfeec44d9b818769dbbf3e9fa.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d001de3cfeec44d9b818769dbbf3e9fa"}}, "title": "Reproductive and Behavior Dysfunction Induced by Maternal Androgen Exposure and Obesity Is Likely Not Gut Microbiome-Mediated.", "authors": [{"family": "Lindheim", "given": "Lisa", "initials": "L", "orcid": "0000-0001-7732-3869", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/175ecb54dd0346a99c78b3a6cf880734.json"}}, {"family": "Manti", "given": "Maria", "initials": "M"}, {"family": "Fornes", "given": "Romina", "initials": "R"}, {"family": "Bashir", "given": "Mina", "initials": "M"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P", "orcid": "0000-0001-8150-4021", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/501677a1262c438aa4077b205bb39a72.json"}}, {"family": "Diaz", "given": "Oscar E", "initials": "OE", "orcid": "0000-0001-7622-7832", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/29eecafe74864c759129278a48114c4a.json"}}, {"family": "Seifert", "given": "Maike", "initials": "M"}, {"family": "Engstrand", "given": "Lars", "initials": "L", "orcid": "0000-0002-7713-2373", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/02d8684df81e42169e613de803446fbf.json"}}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}, {"family": "Obermayer-Pietsch", "given": "Barbara", "initials": "B", "orcid": "0000-0003-3543-1807", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4fb8e7388e6e417c9df0f48759c2b314.json"}}, {"family": "Stener-Victorin", "given": "Elisabet", "initials": "E", "orcid": "0000-0002-3424-1502", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ad1ced271f514141ae3c89e90184c88e.json"}}], "type": "journal article", "published": "2018-12-01", "journal": {"title": "J Endocr Soc", "issn": "2472-1972", "volume": "2", "issue": "12", "pages": "1363-1380", "issn-l": null}, "abstract": "Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder of unclear etiology in women and is characterized by androgen excess, insulin resistance, and mood disorders. The gut microbiome is known to influence conditions closely related with PCOS, and several recent studies have observed changes in the stool microbiome of women with PCOS. The mechanism by which the gut microbiome interacts with PCOS is still unknown. We used a mouse model to investigate if diet-induced maternal obesity and maternal DHT exposure, mimicking the lean and obese PCOS women, cause lasting changes in the gut microbiome of offspring. Fecal microbiome profiles were assessed using Illumina paired-end sequencing of 16S rRNA gene V4 amplicons. We found sex-specific effects of maternal and offspring diet, and maternal DHT exposure on fecal bacterial richness and taxonomic composition. Female offspring exposed to maternal obesity and DHT displayed reproductive dysfunction and anxietylike behavior. Fecal microbiota transplantation from DHT and diet-induced obesity exposed female offspring to wild-type mice did not transfer reproductive dysfunction and did not cause the expected increase in anxietylike behavior in recipients. Maternal obesity and androgen exposure affect the gut microbiome of offspring, but the disrupted estrous cycles and anxietylike behavior are likely not microbiome-mediated.", "doi": "10.1210/js.2018-00266", "pmid": "30534630", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6280317"}, {"db": "pii", "key": "js_201800266"}, {"db": "Dryad", "key": "10.5061/dryad.7h4338m"}], "notes": [], "created": "2026-08-20T12:38:06.284Z", "modified": "2026-08-20T12:38:06.499Z"}, {"entity": "publication", "iuid": "4947804413d44e09bbb713f15edcab47", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4947804413d44e09bbb713f15edcab47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4947804413d44e09bbb713f15edcab47"}}, "title": "\u03b27 integrins contribute to intestinal tumor growth in mice.", "authors": [{"family": "Das", "given": "Srustidhar", "initials": "S"}, {"family": "Do\u00f1as", "given": "Cristian", "initials": "C"}, {"family": "Akeus", "given": "Paulina", "initials": "P"}, {"family": "Quiding-J\u00e4rbrink", "given": "Marianne", "initials": "M"}, {"family": "Mora", "given": "J Rodrigo", "initials": "JR"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c90ee0c3d1904ae0933efe3631a651e0.json"}}], "type": "journal article", "published": "2018-09-20", "journal": {"title": "PLoS ONE", "issn": "1932-6203", "volume": "13", "issue": "9", "pages": "e0204181", "issn-l": "1932-6203"}, "abstract": "The gut homing receptor integrin \u03b14\u03b27 is essential for the migration of pro-inflammatory T cells into the gut mucosa. Since intestinal neoplasia has been associated with chronic inflammation, we investigated whether interfering with gut-homing affects intestinal tumorigenesis. Using chemically induced and spontaneous intestinal tumor models we showed that lack of \u03b27 integrin significantly impairs tumor growth without affecting tumor frequencies, with a mild translatable effect on overall survival. This correlates with human data showing lower MAdCAM-1 expression and disease-free survival in colorectal cancer patients. Thus, paradoxically in contrast to extra-intestinal tumors, blocking migration of immune cells into the gut might have a positive therapeutic effect on intestinal neoplasia.", "doi": "10.1371/journal.pone.0204181", "pmid": "30235302", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6147474"}, {"db": "pii", "key": "PONE-D-18-19194"}], "notes": [], "created": "2026-08-20T12:43:56.483Z", "modified": "2026-08-20T12:43:56.539Z"}]}