{"entity": "researcher", "timestamp": "2026-08-28T00:11:33.591Z", "family": "Fern\u00e1ndez-Alvarez", "given": "Ana Julia", "initials": "AJ", "orcid": "0000-0002-5296-5678", "affiliations": ["Fundaci\u00f3n Instituto Leloir (FIL), Av Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.", "Instituto de Investigaciones Bioqu\u00edmicas de Buenos Aires (IIBBA) - Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET), Av Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c6cdeb4dcc66495698877e16727d785f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c6cdeb4dcc66495698877e16727d785f"}}, "publications": [{"entity": "publication", "iuid": "13748d3dacf94a1385f1005970ce0f51", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/13748d3dacf94a1385f1005970ce0f51.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/13748d3dacf94a1385f1005970ce0f51"}}, "title": "The non-canonical Smoothened-AMPK axis regulates Smaug1 biomolecular condensates.", "authors": [{"family": "Thomas", "given": "Mar\u00eda Gabriela", "initials": "MG", "orcid": "0000-0001-6164-8044", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7ac5790eb4a1483083d9b5e301c1928d.json"}}, {"family": "Fern\u00e1ndez-Alvarez", "given": "Ana Julia", "initials": "AJ", "orcid": "0000-0002-5296-5678", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c6cdeb4dcc66495698877e16727d785f.json"}}, {"family": "Gim\u00e9nez", "given": "Macarena", "initials": "M"}, {"family": "Corvetto Aristarain", "given": "Francisco", "initials": "F"}, {"family": "Cozza", "given": "Lucas Helio", "initials": "LH", "orcid": "0009-0001-4375-7287", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9c490011da9c4e14a40e95f211732cd7.json"}}, {"family": "Pimentel", "given": "Jer\u00f3nimo", "initials": "J", "orcid": "0000-0002-3587-3413", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/127e26a3ed704940a29b6da222755cc1.json"}}, {"family": "Pessoa", "given": "Jo\u00e3o", "initials": "J"}, {"family": "Pascual", "given": "Malena Luc\u00eda", "initials": "ML", "orcid": "0000-0002-9815-1880", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/31d4cf44889840f58de980a4eb6b376c.json"}}, {"family": "Boscaglia", "given": "Lara", "initials": "L"}, {"family": "Habif", "given": "Mart\u00edn", "initials": "M", "orcid": "0000-0001-7548-4247", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5060b798581a4d90a265dc60e024c4ce.json"}}, {"family": "Corbat", "given": "Agust\u00edn", "initials": "A", "orcid": "0000-0001-8068-3486", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b67d947285474c65ac474fdbbc1379b3.json"}}, {"family": "La Spina", "given": "Pablo Ezequiel", "initials": "PE"}, {"family": "Peters", "given": "Tom\u00e1s", "initials": "T", "orcid": "0000-0002-6497-0802", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0df7a7c5db594294b4f7e00a7ba2e0ce.json"}}, {"family": "Bustos", "given": "Diego Mart\u00edn", "initials": "DM", "orcid": "0000-0002-7637-328X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/612063ec62254a28ac4e8d5f593d2888.json"}}, {"family": "Carmo-Fonseca", "given": "Maria", "initials": "M", "orcid": "0000-0002-3402-7143", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a4978c0bf51b4364bf8c11037a1afd9e.json"}}, {"family": "Grecco", "given": "Hern\u00e1n Edgardo", "initials": "HE", "orcid": "0000-0002-1165-4320", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f52a4ed64baf489299632b7b6995b743.json"}}, {"family": "Boccaccio", "given": "Graciela Lidia", "initials": "GL", "orcid": "0000-0003-1349-898X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3a4ce12247b54dd7a3ede6b7f1245a5d.json"}}], "type": "journal article", "published": "2025-03-01", "journal": {"title": "J. Cell. Sci.", "issn": "1477-9137", "volume": "138", "issue": "5", "issn-l": "0021-9533"}, "abstract": "Biomolecular condensates (BMCs) emerge as important players in RNA regulation. The RNA-binding protein Smaug forms cytosolic BMCs in mammals, insects and yeasts and affects mitochondrial function and/or responses to nutrient deprivation. Here, we found that the non-canonical activation of the Smoothened (SMO)-AMPK pathway, which is known to affect energy metabolism, triggers the immediate disassembly of BMCs formed by a number of human and rodent Smaug orthologs, whereas processing bodies remain rather unaltered. A non-phosphorylatable SMO mutant abrogated the effect, involving SMO phosphorylation in human (h)Smaug1 (also known as SAMD4A) BMCs regulation. Three mechanistically different SMO ligands, namely SAG, GSA-10 and cyclopamine, elicited a similar response, which was blocked upon AMPK pharmacological inhibition. Polysome disassembly by puromycin halted Smaug1 BMC dissolution, thus suggesting that unbound transcripts became translationally active. Single-molecule fluorescent in situ hybridization illustrated the release of UQCRC1 mRNA. Finally, Smaug1 is a phosphoprotein bound by 14-3-3 proteins, and the competitive inhibitor difopein blocked the response to non-canonical SMO stimulation. We propose that the regulated condensation and dispersion of Smaug1 BMCs generate translational changes that contribute to metabolic regulation downstream of the non-canonical SMO-AMPK axis.", "doi": "10.1242/jcs.263433", "pmid": "39925132", "labels": [], "xrefs": [{"db": "pii", "key": "365606"}], "notes": [], "created": "2026-08-21T12:42:40.658Z", "modified": "2026-08-21T12:42:41.165Z"}]}