{"entity": "researcher", "timestamp": "2026-09-23T16:35:05.637Z", "family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "affiliations": ["Division of Physiological Chemistry I, Department of Medical Biochemistry and Biophysics , Karolinska Institutet , SE-17 177 Stockholm , Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2"}}, "publications": [{"entity": "publication", "iuid": "179eef258d1745048e037277fdc35daf", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/179eef258d1745048e037277fdc35daf.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/179eef258d1745048e037277fdc35daf"}}, "title": "Anti-Tumoral Treatment with Thioredoxin Reductase 1 Inhibitor Auranofin Fosters Regulatory T Cell and B16F10 Expansion in Mice.", "authors": [{"family": "Bonner", "given": "Michael Y", "initials": "MY", "orcid": "0000-0002-8184-0954", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/71702bac169a4596a8d6befcd859a67d.json"}}, {"family": "Vancsik", "given": "Tamas", "initials": "T", "orcid": "0000-0001-5892-3682", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/55b4d89dc3dd432eaf0a60ce2789ddc6.json"}}, {"family": "Oliveira-Coelho", "given": "Ana", "initials": "A", "orcid": "0000-0001-7783-9750", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9db28e9b73cb420bbd3f07bf30255aad.json"}}, {"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Beusch", "given": "Christian M", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Zeqiraj", "given": "Kejsi", "initials": "K"}, {"family": "Svensson", "given": "Carolin", "initials": "C"}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}, {"family": "Arn\u00e9r", "given": "Elias S J", "initials": "ESJ", "orcid": "0000-0002-4807-6114", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3502128dcae54dfcb6808356694bf4dc.json"}}, {"family": "Holmdahl", "given": "Rikard", "initials": "R", "orcid": "0000-0002-4969-2576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aef6264212044f46a28c24d5fc147438.json"}}], "type": "journal article", "published": "2025-11-11", "journal": {"title": "Antioxidants (Basel)", "issn": "2076-3921", "volume": "14", "issue": "11", "issn-l": null}, "abstract": "Auranofin, an FDA-approved antirheumatic drug and thioredoxin reductase 1 (TXNRD1) inhibitor, has demonstrated anti-tumoral properties, but its immunological effects are not well characterized. Here, we report that auranofin unexpectedly promotes regulatory T cell (Treg) expansion. In a B16F10 melanoma model, auranofin treatment increased lung tumor coverage, IL-10 serum levels, and FOXP3+CD44+CD4+ T cell frequencies. It also altered the proportion of antigen-presenting cells (APCs), increasing B cells and reducing dendritic cells. To test whether Treg expansion occurs independently of tumor antigens, we stimulated T cells ex vivo in lymph node cultures from na\u00efve mice using anti-CD3/CD28, with or without auranofin. Auranofin increased Treg frequency in these cultures, as well as in treated human PBMCs. Similar effects were observed with the TXNRD1 inhibitor TRi-1, suggesting a ROS-dependent mechanism. Using mice with conditional expression of neutrophil cytosolic factor 1 (NCF1), we found that both TXNRD1 inhibition and APC-specific NCF1-NOX2-ROS expression enhanced tumor burden and Treg expansion. Alternatively, sorted T cells from mice harboring conditional TXNRD1 knockouts showed reduced FOXP3 and GITR expression in the na\u00efve state and reduced tumor burden when challenged with B16F10. These data suggest TXNRD1 inhibitors likely drive Treg expansion by elevating ROS levels in APCs during T cell priming and less by intrinsic Treg TXNRD1 blockade. Our findings reveal a paradoxical immunosuppressive effect of TXNRD1 inhibitors that may contribute to their limited efficacy in immunocompetent cancer models. This work provides mechanistic insight and underscores the need to consider Treg-mediated immune suppression when designing TXNRD1-targeted therapies.", "doi": "10.3390/antiox14111351", "pmid": "41300507", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12649656"}, {"db": "pii", "key": "antiox14111351"}], "notes": [], "created": "2026-09-23T13:19:31.188Z", "modified": "2026-09-23T13:19:31.358Z"}, {"entity": "publication", "iuid": "13d75815a3a84344b2331e40d6d59edc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/13d75815a3a84344b2331e40d6d59edc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/13d75815a3a84344b2331e40d6d59edc"}}, "title": "System-wide profiling by proteome integral solubility alteration assay of drug residence times for target characterization", "authors": [{"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Beusch", "given": "Christian M", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Meng", "given": "Zhaowei", "initials": "Z", "orcid": "0000-0002-7721-2795", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/39ee47850dd643bbbc22d54f8e5419a3.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "posted-content", "published": "2022-06-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.06.27.497697", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T15:19:12.937Z", "modified": "2026-09-23T15:19:12.966Z"}, {"entity": "publication", "iuid": "6d70455ae74e439fbd4235be9faa3c1e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/6d70455ae74e439fbd4235be9faa3c1e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/6d70455ae74e439fbd4235be9faa3c1e"}}, "title": "An integrative proteomics method identifies a regulator of translation during stem cell maintenance and differentiation.", "authors": [{"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Beusch", "given": "Christian M", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Saei", "given": "Amir A", "initials": "AA", "orcid": "0000-0002-2639-6328", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf703f0f2d44b14846ea1d9811b6e0d.json"}}, {"family": "Aoun", "given": "Mike", "initials": "M"}, {"family": "Moruzzi", "given": "Noah", "initials": "N"}, {"family": "Coelho", "given": "Ana", "initials": "A"}, {"family": "Leijten", "given": "Niels", "initials": "N", "orcid": "0000-0002-7305-7249", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da29aa1ce79d48bfa04fcc9ef6dcaa37.json"}}, {"family": "Nordenskj\u00f6ld", "given": "Magnus", "initials": "M"}, {"family": "Micke", "given": "Patrick", "initials": "P", "orcid": "0000-0003-1210-5961", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8b4b9d0f748440e19db93d4543bbe25f.json"}}, {"family": "Maltseva", "given": "Diana", "initials": "D", "orcid": "0000-0002-4960-5348", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9228e30610f24e4ea3cfb9baea4a551e.json"}}, {"family": "Tonevitsky", "given": "Alexander G", "initials": "AG"}, {"family": "Millischer", "given": "Vincent", "initials": "V"}, {"family": "Carlos Villaescusa", "given": "J", "initials": "J"}, {"family": "Kadekar", "given": "Sandeep", "initials": "S"}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a402c63fbe4f478daa286177f031b923.json"}}, {"family": "Altynbekova", "given": "Kamilya", "initials": "K"}, {"family": "Kel", "given": "Alexander", "initials": "A"}, {"family": "Berggren", "given": "Per-Olof", "initials": "PO"}, {"family": "Simonson", "given": "Oscar", "initials": "O"}, {"family": "Grinnemo", "given": "Karl-Henrik", "initials": "KH"}, {"family": "Holmdahl", "given": "Rikard", "initials": "R", "orcid": "0000-0002-4969-2576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/aef6264212044f46a28c24d5fc147438.json"}}, {"family": "Rodin", "given": "Sergey", "initials": "S", "orcid": "0000-0002-6954-1986", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0befd77d36cf48ffbb5a4a04c0641a58.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "journal article", "published": "2021-11-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "6558", "issn-l": "2041-1723"}, "abstract": "Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro.", "doi": "10.1038/s41467-021-26879-4", "pmid": "34772928", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC8590018"}, {"db": "pii", "key": "10.1038/s41467-021-26879-4"}], "notes": [], "created": "2026-09-23T08:50:28.960Z", "modified": "2026-09-23T08:50:29.205Z"}, {"entity": "publication", "iuid": "c28fb876d1334187af97136f2fff26d0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c28fb876d1334187af97136f2fff26d0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c28fb876d1334187af97136f2fff26d0"}}, "title": "System-wide identification and prioritization of enzyme substrates by thermal analysis.", "authors": [{"family": "Saei", "given": "Amir Ata", "initials": "AA", "orcid": "0000-0002-2639-6328", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf703f0f2d44b14846ea1d9811b6e0d.json"}}, {"family": "Beusch", "given": "Christian M", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Wells", "given": "Juan Astorga", "initials": "JA", "orcid": "0000-0003-1017-8841", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/855ef6e5db6f4791a52eeae9ffb95a30.json"}}, {"family": "Gharibi", "given": "Hassan", "initials": "H", "orcid": "0000-0002-3072-4929", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5825ec93851a42618425b67a5bbfbf3f.json"}}, {"family": "Meng", "given": "Zhaowei", "initials": "Z"}, {"family": "Chernobrovkin", "given": "Alexey", "initials": "A", "orcid": "0000-0001-7709-0161", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/011675da8d7a4fd68fbe5ce03227978d.json"}}, {"family": "Rodin", "given": "Sergey", "initials": "S"}, {"family": "N\u00e4reoja", "given": "Katja", "initials": "K"}, {"family": "Thorsell", "given": "Ann-Gerd", "initials": "AG"}, {"family": "Karlberg", "given": "Tobias", "initials": "T"}, {"family": "Cheng", "given": "Qing", "initials": "Q"}, {"family": "Lundstr\u00f6m", "given": "Susanna L", "initials": "SL"}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a402c63fbe4f478daa286177f031b923.json"}}, {"family": "V\u00e9gv\u00e1ri", "given": "\u00c1kos", "initials": "\u00c1", "orcid": "0000-0002-1287-0906", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/724d65ef088147c4988093b2b6b5f318.json"}}, {"family": "Arn\u00e9r", "given": "Elias S J", "initials": "ESJ", "orcid": "0000-0002-4807-6114", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3502128dcae54dfcb6808356694bf4dc.json"}}, {"family": "Sch\u00fcler", "given": "Herwig", "initials": "H", "orcid": "0000-0003-4059-3501", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6a66d04210ea41c2bbb0a5ee187c7b76.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "journal article", "published": "2021-02-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "12", "issue": "1", "pages": "1296", "issn-l": "2041-1723"}, "abstract": "Despite the immense importance of enzyme-substrate reactions, there is a lack of general and unbiased tools for identifying and prioritizing substrate proteins that are modified by the enzyme on the structural level. Here we describe a high-throughput unbiased proteomics method called System-wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that the enzymatic post-translational modification of substrate proteins is likely to change their thermal stability. In our proof-of-concept studies, SIESTA successfully identifies several known and novel substrate candidates for selenoprotein thioredoxin reductase 1, protein kinase B (AKT1) and poly-(ADP-ribose) polymerase-10 systems. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, opening opportunities to investigate the effect of post-translational modifications on signal transduction and facilitate drug discovery.", "doi": "10.1038/s41467-021-21540-6", "pmid": "33637753", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC7910609"}, {"db": "pii", "key": "10.1038/s41467-021-21540-6"}], "notes": [], "created": "2026-09-23T08:34:01.574Z", "modified": "2026-09-23T08:34:01.919Z"}, {"entity": "publication", "iuid": "3296f3147d3b4070a92e1424a7f1b82d", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3296f3147d3b4070a92e1424a7f1b82d.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3296f3147d3b4070a92e1424a7f1b82d"}}, "title": "ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery.", "authors": [{"family": "Saei", "given": "Amir Ata", "initials": "AA", "orcid": "0000-0002-2639-6328", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ebf703f0f2d44b14846ea1d9811b6e0d.json"}}, {"family": "Beusch", "given": "Christian Michel", "initials": "CM", "orcid": "0000-0001-9100-8283", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fbb4771768354080ab5292c96fee0812.json"}}, {"family": "Chernobrovkin", "given": "Alexey", "initials": "A", "orcid": "0000-0001-7709-0161", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/011675da8d7a4fd68fbe5ce03227978d.json"}}, {"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Zhang", "given": "Bo", "initials": "B", "orcid": "0000-0001-8890-8416", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fab1ab9de65a430b97f0a84901798d6e.json"}}, {"family": "Tokat", "given": "\u00dclk\u00fc G\u00fcler", "initials": "\u00dcG"}, {"family": "Stergiou", "given": "Eleni", "initials": "E", "orcid": "0000-0001-9115-4985", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2912f51ad92a49069667f3947a32562e.json"}}, {"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a402c63fbe4f478daa286177f031b923.json"}}, {"family": "V\u00e9gv\u00e1ri", "given": "\u00c1kos", "initials": "\u00c1", "orcid": "0000-0002-1287-0906", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/724d65ef088147c4988093b2b6b5f318.json"}}, {"family": "Zubarev", "given": "Roman A", "initials": "RA"}], "type": "journal article", "published": "2019-12-16", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "10", "issue": "1", "pages": "5715", "issn-l": "2041-1723"}, "abstract": "Deconvolution of targets and action mechanisms of anticancer compounds is fundamental in drug development. Here, we report on ProTargetMiner as a publicly available expandable proteome signature library of anticancer molecules in cancer cell lines. Based on 287 A549 adenocarcinoma proteomes affected by 56 compounds, the main dataset contains 7,328 proteins and 1,307,859 refined protein-drug pairs. These proteomic signatures cluster by compound targets and action mechanisms. The targets and mechanistic proteins are deconvoluted by partial least square modeling, provided through the website http://protargetminer.genexplain.com. For 9 molecules representing the most diverse mechanisms and the common cancer cell lines MCF-7, RKO and A549, deep proteome datasets are obtained. Combining data from the three cell lines highlights common drug targets and cell-specific differences. The database can be easily extended and merged with new compound signatures. ProTargetMiner serves as a chemical proteomics resource for the cancer research community, and can become a valuable tool in drug discovery.", "doi": "10.1038/s41467-019-13582-8", "pmid": "31844049", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6915695"}, {"db": "pii", "key": "10.1038/s41467-019-13582-8"}], "notes": [], "created": "2026-09-23T07:49:29.913Z", "modified": "2026-09-23T07:49:30.074Z"}, {"entity": "publication", "iuid": "d4e52f3e02c14b2782f525d9296d61c0", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d4e52f3e02c14b2782f525d9296d61c0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d4e52f3e02c14b2782f525d9296d61c0"}}, "title": "Proteome Integral Solubility Alteration: A High-Throughput Proteomics Assay for Target Deconvolution.", "authors": [{"family": "Gaetani", "given": "Massimiliano", "initials": "M", "orcid": "0000-0001-5610-0797", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a402c63fbe4f478daa286177f031b923.json"}}, {"family": "Sabatier", "given": "Pierre", "initials": "P", "orcid": "0000-0002-2734-1791", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c4cf83f2cf534d7eb308dab89d6460e2.json"}}, {"family": "Saei", "given": "Amir A", "initials": "AA"}, {"family": "Beusch", "given": "Christian M", "initials": "CM"}, {"family": "Yang", "given": "Zhe", "initials": "Z"}, {"family": "Lundstr\u00f6m", "given": "Susanna L", "initials": "SL"}, {"family": "Zubarev", "given": "Roman A", "initials": "RA", "orcid": "0000-0001-9839-2089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e3f9910c1ff434c8056bdecf537e9ef.json"}}], "type": "journal article", "published": "2019-11-01", "journal": {"title": "J. Proteome Res.", "issn": "1535-3907", "volume": "18", "issue": "11", "pages": "4027-4037", "issn-l": "1535-3893"}, "abstract": "Various agents, including drugs as well as nonmolecular stimuli, induce alterations in the physicochemical properties of proteins in cell lysates, living cells, and organisms. These alterations can be probed by applying a stability- and solubility-modifying factor, such as elevated temperature, to a varying degree. As a second dimension of variation, drug concentration or agent intensity/concentration can be used. Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by 1 to 2 orders of magnitude for an unlimited number of factor variation points in such a scheme. The consumption of the compound and biological material decreases in PISA by the same factor. We envision widespread use of the PISA approach in chemical biology and drug development.", "doi": "10.1021/acs.jproteome.9b00500", "pmid": "31545609", "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T06:38:28.503Z", "modified": "2026-09-23T06:38:28.585Z"}]}