{"entity": "researcher", "timestamp": "2026-08-22T07:48:28.969Z", "family": "Campaner", "given": "Stefano", "initials": "S", "orcid": "0000-0003-4547-6849", "affiliations": ["Center for Genomic Science of IIT, CGS@SEMM (Istituto Italiano di Tecnologia at European School of Molecular Medicine), Fondazione Istituto Italiano di Tecnologia (IIT), 20139, Milan, Italy. stefano.campaner@iit.it.", "Department of Molecular Medicine, University of Padua, Padua, Italy. stefano.campaner@iit.it."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c47263351cdd41b4a2c3a95f0430a1ef.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c47263351cdd41b4a2c3a95f0430a1ef"}}, "publications": [{"entity": "publication", "iuid": "4ae6c165f93f42819c7d2c062d672485", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4ae6c165f93f42819c7d2c062d672485.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4ae6c165f93f42819c7d2c062d672485"}}, "title": "Correction: Senataxin prevents replicative stress induced by the Myc oncogene.", "authors": [{"family": "Sberna", "given": "Silvia", "initials": "S"}, {"family": "Filipuzzi", "given": "Marco", "initials": "M", "orcid": "0000-0002-4584-4114", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/78a755316bb4425eb77ed7a0f8035a48.json"}}, {"family": "Bianchi", "given": "Nicola", "initials": "N"}, {"family": "Croci", "given": "Ottavio", "initials": "O"}, {"family": "Fardella", "given": "Federica", "initials": "F"}, {"family": "Soriani", "given": "Chiara", "initials": "C"}, {"family": "Rohban", "given": "Sara", "initials": "S"}, {"family": "Carnevali", "given": "Sara", "initials": "S"}, {"family": "Albertini", "given": "Alessandra Alberta", "initials": "AA"}, {"family": "Crosetto", "given": "Nicola", "initials": "N", "orcid": "0000-0002-3019-6978", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b1cef698ae749749b4017dbacb9e3db.json"}}, {"family": "Rodighiero", "given": "Simona", "initials": "S"}, {"family": "Chiesa", "given": "Arianna", "initials": "A"}, {"family": "Curti", "given": "Laura", "initials": "L"}, {"family": "Campaner", "given": "Stefano", "initials": "S", "orcid": "0000-0003-4547-6849", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c47263351cdd41b4a2c3a95f0430a1ef.json"}}], "type": "published erratum", "published": "2025-05-12", "journal": {"title": "Cell Death Dis", "issn": "2041-4889", "volume": "16", "issue": "1", "pages": "377", "issn-l": "2041-4889"}, "abstract": null, "doi": "10.1038/s41419-025-07671-4", "pmid": "40355407", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12069646"}, {"db": "pii", "key": "10.1038/s41419-025-07671-4"}], "notes": [], "created": "2026-08-21T11:47:47.564Z", "modified": "2026-08-21T11:47:47.618Z"}, {"entity": "publication", "iuid": "cb507c8a581b43a090f6433368a7e246", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/cb507c8a581b43a090f6433368a7e246.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/cb507c8a581b43a090f6433368a7e246"}}, "title": "Senataxin prevents replicative stress induced by the Myc oncogene.", "authors": [{"family": "Sberna", "given": "Silvia", "initials": "S"}, {"family": "Filipuzzi", "given": "Marco", "initials": "M", "orcid": "0000-0002-4584-4114", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/78a755316bb4425eb77ed7a0f8035a48.json"}}, {"family": "Bianchi", "given": "Nicola", "initials": "N"}, {"family": "Croci", "given": "Ottavio", "initials": "O"}, {"family": "Fardella", "given": "Federica", "initials": "F"}, {"family": "Soriani", "given": "Chiara", "initials": "C"}, {"family": "Rohban", "given": "Sara", "initials": "S"}, {"family": "Carnevali", "given": "Sara", "initials": "S"}, {"family": "Albertini", "given": "Alessandra Alberta", "initials": "AA"}, {"family": "Crosetto", "given": "Nicola", "initials": "N", "orcid": "0000-0002-3019-6978", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b1cef698ae749749b4017dbacb9e3db.json"}}, {"family": "Rodighiero", "given": "Simona", "initials": "S"}, {"family": "Chiesa", "given": "Arianna", "initials": "A"}, {"family": "Curti", "given": "Laura", "initials": "L"}, {"family": "Campaner", "given": "Stefano", "initials": "S", "orcid": "0000-0003-4547-6849", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c47263351cdd41b4a2c3a95f0430a1ef.json"}}], "type": "journal article", "published": "2025-03-19", "journal": {"title": "Cell Death Dis", "issn": "2041-4889", "volume": "16", "issue": "1", "pages": "187", "issn-l": "2041-4889"}, "abstract": "Replicative stress (RS) is emerging as a promising therapeutic target in oncology, yet full exploitation of its potential requires a detailed understanding of the mechanisms and genes involved. Here, we investigated the RNA helicase Senataxin (SETX), an enzyme that resolves RNA-DNA hybrids and R-loops, to address its role in preventing RS by oncogenic Myc. Upon Myc activation, silencing of SETX led to selective engagement of the DNA damage response (DDR) and robust cytotoxicity. Pharmacological dissection of the upstream kinases regulating the DDR uncovered a protective role of the ATR pathway, that once inhibited, boosted SETX driven-DDR. While SETX loss did not lead to a genome-wide increase of R-loops, mechanistic analyses revealed enhanced R-loops localized at DDR-foci and newly replicated genomic loci, compatible with a selective role of SETX in resolving RNA-DNA hybrids to alleviate Myc-induced RS. Genome-wide mapping of DNA double-strand breaks confirmed that SETX silencing exacerbated DNA damage at transcription-replication conflict (TRC) regions at early replicated sites. We propose that SETX prevents Myc-induced TRCs by resolving transcription-associated R-loops that encounter the replisome. The identification of SETX as a genetic liability of oncogenic Myc opens up new therapeutic options against aggressive Myc-driven tumors.", "doi": "10.1038/s41419-025-07485-4", "pmid": "40108134", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11923212"}, {"db": "pii", "key": "10.1038/s41419-025-07485-4"}], "notes": [], "created": "2026-08-21T11:47:45.585Z", "modified": "2026-08-21T11:47:45.700Z"}, {"entity": "publication", "iuid": "e06251bc320840929b8cb305af8d23bd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e06251bc320840929b8cb305af8d23bd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e06251bc320840929b8cb305af8d23bd"}}, "title": "CDK12 controls transcription at damaged genes and prevents MYC-induced transcription-replication conflicts.", "authors": [{"family": "Curti", "given": "Laura", "initials": "L"}, {"family": "Rohban", "given": "Sara", "initials": "S"}, {"family": "Bianchi", "given": "Nicola", "initials": "N"}, {"family": "Croci", "given": "Ottavio", "initials": "O"}, {"family": "Andronache", "given": "Adrian", "initials": "A", "orcid": "0009-0002-6961-7829", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b275873bf4b476182129d79791ac659.json"}}, {"family": "Barozzi", "given": "Sara", "initials": "S"}, {"family": "Mattioli", "given": "Michela", "initials": "M"}, {"family": "Ricci", "given": "Fernanda", "initials": "F"}, {"family": "Pastori", "given": "Elena", "initials": "E"}, {"family": "Sberna", "given": "Silvia", "initials": "S"}, {"family": "Bellotti", "given": "Simone", "initials": "S"}, {"family": "Accialini", "given": "Anna", "initials": "A"}, {"family": "Ballarino", "given": "Roberto", "initials": "R", "orcid": "0000-0001-7812-0940", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7885bdf463cf41538ea08a35898c1cae.json"}}, {"family": "Crosetto", "given": "Nicola", "initials": "N", "orcid": "0000-0002-3019-6978", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/0b1cef698ae749749b4017dbacb9e3db.json"}}, {"family": "Wade", "given": "Mark", "initials": "M"}, {"family": "Parazzoli", "given": "Dario", "initials": "D", "orcid": "0000-0001-8176-9775", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5e797a3ebe9546cc8a398d326dbe4b91.json"}}, {"family": "Campaner", "given": "Stefano", "initials": "S", "orcid": "0000-0003-4547-6849", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c47263351cdd41b4a2c3a95f0430a1ef.json"}}], "type": "journal article", "published": "2024-08-18", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "7100", "issn-l": "2041-1723"}, "abstract": "The identification of genes involved in replicative stress is key to understanding cancer evolution and to identify therapeutic targets. Here, we show that CDK12 prevents transcription-replication conflicts (TRCs) and the activation of cytotoxic replicative stress upon deregulation of the MYC oncogene. CDK12 was recruited at damaged genes by PARP-dependent DDR-signaling and elongation-competent RNAPII, to repress transcription. Either loss or chemical inhibition of CDK12 led to DDR-resistant transcription of damaged genes. Loss of CDK12 exacerbated TRCs in MYC-overexpressing cells and led to the accumulation of double-strand DNA breaks, occurring between co-directional early-replicating regions and transcribed genes. Overall, our data demonstrate that CDK12 protects genome integrity by repressing transcription of damaged genes, which is required for proper resolution of DSBs at oncogene-induced TRCs. This provides a rationale that explains both how CDK12 deficiency can promote tandem duplications of early-replicated regions during tumor evolution, and how CDK12 targeting can exacerbate replicative-stress in tumors.", "doi": "10.1038/s41467-024-51229-5", "pmid": "39155303", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11330984"}, {"db": "pii", "key": "10.1038/s41467-024-51229-5"}], "notes": [], "created": "2026-08-21T11:49:55.780Z", "modified": "2026-08-21T11:49:55.957Z"}]}