{"entity": "researcher", "timestamp": "2026-08-20T20:49:06.937Z", "family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "affiliations": ["Department of Oncology and Pathology, Karolinska Institute, Science for Life Laboratory, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef"}}, "publications": [{"entity": "publication", "iuid": "e450667eac9b4d74b78acd579fd31860", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e450667eac9b4d74b78acd579fd31860.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e450667eac9b4d74b78acd579fd31860"}}, "title": "A Multi-Omic Phenobank Reveals Axes of Glioblastoma Growth, Invasion, and Therapeutic Vulnerability", "authors": [{"family": "Krona", "given": "Cecilia", "initials": "C", "orcid": "0000-0001-6458-1949", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/847502424e244d4783f34f88545d7998.json"}}, {"family": "Kundu", "given": "Soumi", "initials": "S"}, {"family": "Rosen", "given": "Emil", "initials": "E"}, {"family": "Kruse", "given": "Filippa", "initials": "F"}, {"family": "Skepp\u00e5s", "given": "Madeleine", "initials": "M"}, {"family": "Baba\u010di\u0107", "given": "Haris", "initials": "H"}, {"family": "Larsson", "given": "Ida", "initials": "I", "orcid": "0000-0001-5422-4243", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/de0a82c0016b482e875e5b26cbb0c551.json"}}, {"family": "Elfineh", "given": "Ludmila", "initials": "L"}, {"family": "Schuan L\u00fc", "given": "Maya Jeje", "initials": "MJ"}, {"family": "Conde", "given": "Maria Escriva", "initials": "ME"}, {"family": "Elgendy", "given": "Ramy", "initials": "R", "orcid": "0000-0002-2592-3448", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a1f51cdd18014c0d80b5545846bb53ff.json"}}, {"family": "Dave", "given": "Zankruti", "initials": "Z", "orcid": "0000-0002-3786-0747", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f30e0019473641bc9f300ad3e8c60c03.json"}}, {"family": "Doroszko", "given": "Milena", "initials": "M"}, {"family": "Rut-Halldorsdottir", "given": "Katrin", "initials": "K"}, {"family": "Cao", "given": "Xiaofang", "initials": "X"}, {"family": "Ramachandra", "given": "Rashmi", "initials": "R"}, {"family": "Olausson", "given": "Karl Holmberg", "initials": "KH"}, {"family": "Nilsson", "given": "Mats", "initials": "M"}, {"family": "Weischenfeldt", "given": "Joachim", "initials": "J"}, {"family": "Wikstr\u00f6m", "given": "Johan", "initials": "J"}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Sundstr\u00f6m", "given": "Anders", "initials": "A"}, {"family": "Uppman", "given": "Irem", "initials": "I", "orcid": "0000-0003-3602-5544", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8da698d30e754596a074851a63142df0.json"}}, {"family": "Mangukiya", "given": "Hitesh Bhagavanbhai", "initials": "HB", "orcid": "0000-0002-8460-4850", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79de6b3589bf418fb8bcf4047ad866c6.json"}}, {"family": "Nelander", "given": "Sven", "initials": "S", "orcid": "0000-0003-1758-1262", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4f7491aab21f419dbe39276d1ef80077.json"}}], "type": "posted-content", "published": "2025-03-28", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2025.03.25.645260", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T11:01:48.931Z", "modified": "2026-08-20T11:01:49.214Z"}, {"entity": "publication", "iuid": "ae97c4dc45534c65b9afcbf66497d86b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ae97c4dc45534c65b9afcbf66497d86b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ae97c4dc45534c65b9afcbf66497d86b"}}, "title": "Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures.", "authors": [{"family": "Baba\u010di\u0107", "given": "Haris", "initials": "H", "orcid": "0000-0003-0813-0005", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/69dbef8e901c4fafb00a18b6d7722acd.json"}}, {"family": "Galardi", "given": "Silvia", "initials": "S"}, {"family": "Umer", "given": "Husen M", "initials": "HM"}, {"family": "Hellstr\u00f6m", "given": "Mats", "initials": "M"}, {"family": "Uhrbom", "given": "Lene", "initials": "L"}, {"family": "Maturi", "given": "Nagaprathyusha", "initials": "N"}, {"family": "Cardinali", "given": "Deborah", "initials": "D"}, {"family": "Pellegatta", "given": "Serena", "initials": "S"}, {"family": "Michienzi", "given": "Alessandro", "initials": "A"}, {"family": "Trevisi", "given": "Gianluca", "initials": "G"}, {"family": "Mangiola", "given": "Annunziato", "initials": "A"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Ciafr\u00e8", "given": "Silvia Anna", "initials": "SA", "orcid": "0000-0001-6467-8396", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/19262b98fa97433f8b9c900218751748.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}], "type": "journal article", "published": "2023-02-00", "journal": {"title": "Mol Oncol", "issn": "1878-0261", "volume": "17", "issue": "2", "pages": "238-260", "issn-l": "1574-7891"}, "abstract": "Glioblastoma (GBM) cancer stem cells (GSCs) contribute to GBM's origin, recurrence, and resistance to treatment. However, the understanding of how mRNA expression patterns of GBM subtypes are reflected at global proteome level in GSCs is limited. To characterize protein expression in GSCs, we performed in-depth proteogenomic analysis of patient-derived GSCs by RNA-sequencing and mass-spectrometry. We quantified > 10 000 proteins in two independent GSC panels and propose a GSC-associated proteomic signature characterizing two distinct phenotypic conditions; one defined by proteins upregulated in proneural and classical GSCs (GPC-like), and another by proteins upregulated in mesenchymal GSCs (GM-like). The GM-like protein set in GBM tissue was associated with necrosis, recurrence, and worse overall survival. Through proteogenomics, we discovered 252 non-canonical peptides in the GSCs, i.e., protein sequences that are variant or derive from genome regions previously considered non-protein-coding, including variants of the heterogeneous ribonucleoproteins implicated in RNA splicing. In summary, GSCs express two protein sets that have an inverse association with clinical outcomes in GBM. The discovery of non-canonical protein sequences questions existing gene models and pinpoints new protein targets for research in GBM.", "doi": "10.1002/1878-0261.13355", "pmid": "36495079", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9892829"}], "notes": [], "created": "2026-08-20T06:26:51.885Z", "modified": "2026-08-20T06:26:52.155Z"}, {"entity": "publication", "iuid": "d3fd353ba298423eac45e5659ebf44aa", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d3fd353ba298423eac45e5659ebf44aa.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d3fd353ba298423eac45e5659ebf44aa"}}, "title": "Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures", "authors": [{"family": "Baba\u010di\u0107", "given": "Haris", "initials": "H", "orcid": "0000-0003-0813-0005", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/69dbef8e901c4fafb00a18b6d7722acd.json"}}, {"family": "Galardi", "given": "Silvia", "initials": "S"}, {"family": "Umer", "given": "Husen M", "initials": "HM", "orcid": "0000-0003-3971-2462", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/922625c410444833a263244d0313980e.json"}}, {"family": "Cardinali", "given": "Deborah", "initials": "D"}, {"family": "Pellegatta", "given": "Serena", "initials": "S"}, {"family": "Hellstr\u00f6m", "given": "Mats", "initials": "M"}, {"family": "Uhrbom", "given": "Lene", "initials": "L", "orcid": "0000-0001-8595-5698", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bf57deb01a9340b6b5b2174195b6a549.json"}}, {"family": "Maturi", "given": "Nagaprathyusha", "initials": "N"}, {"family": "Michienzi", "given": "Alessandro", "initials": "A"}, {"family": "Trevisi", "given": "Gianluca", "initials": "G"}, {"family": "Mangiola", "given": "Annunziato", "initials": "A"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/561efcf32e2648c8a10fee692fc4e908.json"}}, {"family": "Ciafr\u00e8", "given": "Silvia Anna", "initials": "SA"}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}], "type": "posted-content", "published": "2022-02-10", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2022.02.06.479313", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:09:24.426Z", "modified": "2026-08-20T10:09:24.500Z"}, {"entity": "publication", "iuid": "209b1e6bec59490f9922fab7244b452a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/209b1e6bec59490f9922fab7244b452a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/209b1e6bec59490f9922fab7244b452a"}}, "title": "Advances and Utility of the Human Plasma Proteome.", "authors": [{"family": "Deutsch", "given": "Eric W", "initials": "EW", "orcid": "0000-0001-8732-0928", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2463c206245a4dbb979cad9d201d73c4.json"}}, {"family": "Omenn", "given": "Gilbert S", "initials": "GS", "orcid": "0000-0002-8976-6074", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8271c48d1bc6477ca3ba90d05d3854ad.json"}}, {"family": "Sun", "given": "Zhi", "initials": "Z", "orcid": "0000-0003-3324-6851", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d5076a80c046441482472dea34610eb6.json"}}, {"family": "Maes", "given": "Michal", "initials": "M", "orcid": "0000-0002-7406-3982", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/81e9fc09132c4e5685870c00b35717ea.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Palaniappan", "given": "Krishnan K", "initials": "KK", "orcid": "0000-0003-0203-9016", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9ad180add9d14755b44834d00ea88fb4.json"}}, {"family": "Letunica", "given": "Natasha", "initials": "N", "orcid": "0000-0003-3604-1536", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ca1b9a786b064d6aa7b8c68331fc25f0.json"}}, {"family": "Vandenbrouck", "given": "Yves", "initials": "Y", "orcid": "0000-0002-1292-373X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ed616edf97c454aa6b79c56e565ce64.json"}}, {"family": "Brun", "given": "Virginie", "initials": "V", "orcid": "0000-0002-9098-8707", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/075e825949ea4031bbcf6a2449ce1051.json"}}, {"family": "Tao", "given": "Sheng-Ce", "initials": "SC", "orcid": "0000-0002-9210-1823", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/95665a9e4ff6496dbe47132ddf08ef3b.json"}}, {"family": "Yu", "given": "Xiaobo", "initials": "X"}, {"family": "Geyer", "given": "Philipp E", "initials": "PE", "orcid": "0000-0001-7980-4826", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9d69184685884e28ac1557b8bf354920.json"}}, {"family": "Ignjatovic", "given": "Vera", "initials": "V", "orcid": "0000-0002-5548-5813", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8fa9f5752da349a79a4969866f014edb.json"}}, {"family": "Moritz", "given": "Robert L", "initials": "RL", "orcid": "0000-0002-3216-9447", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/505eb37634de49d4b6a29f41c18f142b.json"}}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}], "type": "journal article", "published": "2021-12-03", "journal": {"title": "J. Proteome Res.", "issn": "1535-3907", "volume": "20", "issue": "12", "pages": "5241-5263", "issn-l": "1535-3893"}, "abstract": "The study of proteins circulating in blood offers tremendous opportunities to diagnose, stratify, or possibly prevent diseases. With recent technological advances and the urgent need to understand the effects of COVID-19, the proteomic analysis of blood-derived serum and plasma has become even more important for studying human biology and pathophysiology. Here we provide views and perspectives about technological developments and possible clinical applications that use mass-spectrometry(MS)- or affinity-based methods. We discuss examples where plasma proteomics contributed valuable insights into SARS-CoV-2 infections, aging, and hemostasis and the opportunities offered by combining proteomics with genetic data. As a contribution to the Human Proteome Organization (HUPO) Human Plasma Proteome Project (HPPP), we present the Human Plasma PeptideAtlas build 2021-07 that comprises 4395 canonical and 1482 additional nonredundant human proteins detected in 240 MS-based experiments. In addition, we report the new Human Extracellular Vesicle PeptideAtlas 2021-06, which comprises five studies and 2757 canonical proteins detected in extracellular vesicles circulating in blood, of which 74% (2047) are in common with the plasma PeptideAtlas. Our overview summarizes the recent advances, impactful applications, and ongoing challenges for translating plasma proteomics into utility for precision medicine.", "doi": "10.1021/acs.jproteome.1c00657", "pmid": "34672606", "labels": [], "xrefs": [{"db": "mid", "key": "NIHMS1833436"}, {"db": "pmc", "key": "PMC9469506"}], "notes": [], "created": "2026-08-20T08:11:22.145Z", "modified": "2026-08-20T08:11:22.712Z"}, {"entity": "publication", "iuid": "3113ee5c7c5e42c8a0831e62b2e2c8f6", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/3113ee5c7c5e42c8a0831e62b2e2c8f6.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/3113ee5c7c5e42c8a0831e62b2e2c8f6"}}, "title": "Early symptoms and sensations as predictors of lung cancer: a machine learning multivariate model.", "authors": [{"family": "Levitsky", "given": "Adrian", "initials": "A", "orcid": "0000-0003-4821-0691", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/56d2c32efba54cfe88679bc007c67854.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Bernhardson", "given": "Britt-Marie", "initials": "BM"}, {"family": "Forshed", "given": "Jenny", "initials": "J"}, {"family": "K\u00f6lbeck", "given": "Karl", "initials": "K"}, {"family": "Olin", "given": "Maria", "initials": "M"}, {"family": "Henriksson", "given": "Roger", "initials": "R"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/561efcf32e2648c8a10fee692fc4e908.json"}}, {"family": "Tishelman", "given": "Carol", "initials": "C"}, {"family": "Eriksson", "given": "Lars E", "initials": "LE", "orcid": "0000-0001-5121-5325", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a6583e7b1b0d4dc38d27eb8ad50f1add.json"}}], "type": "journal article", "published": "2019-11-11", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "9", "issue": "1", "pages": "16504", "issn-l": "2045-2322"}, "abstract": "The aim of this study was to identify a combination of early predictive symptoms/sensations attributable to primary lung cancer (LC). An interactive e-questionnaire comprised of pre-diagnostic descriptors of first symptoms/sensations was administered to patients referred for suspected LC. Respondents were included in the present analysis only if they later received a primary LC diagnosis or had no cancer; and inclusion of each descriptor required \u22654 observations. Fully-completed data from 506/670 individuals later diagnosed with primary LC (n = 311) or no cancer (n = 195) were modelled with orthogonal projections to latent structures (OPLS). After analysing 145/285 descriptors, meeting inclusion criteria, through randomised seven-fold cross-validation (six-fold training set: n = 433; test set: n = 73), 63 provided best LC prediction. The most-significant LC-positive descriptors included a cough that varied over the day, back pain/aches/discomfort, early satiety, appetite loss, and having less strength. Upon combining the descriptors with the background variables current smoking, a cold/flu or pneumonia within the past two years, female sex, older age, a history of COPD (positive LC-association); antibiotics within the past two years, and a history of pneumonia (negative LC-association); the resulting 70-variable model had accurate cross-validated test set performance: area under the ROC curve = 0.767 (descriptors only: 0.736/background predictors only: 0.652), sensitivity = 84.8% (73.9/76.1%, respectively), specificity = 55.6% (66.7/51.9%, respectively). In conclusion, accurate prediction of LC was found through 63 early symptoms/sensations and seven background factors. Further research and precision in this model may lead to a tool for referral and LC diagnostic decision-making.", "doi": "10.1038/s41598-019-52915-x", "pmid": "31712735", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6848139"}, {"db": "pii", "key": "10.1038/s41598-019-52915-x"}], "notes": [], "created": "2026-08-20T09:04:42.059Z", "modified": "2026-08-20T09:04:42.195Z"}, {"entity": "publication", "iuid": "e4c03509339a4a7487c366bc3827bc47", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e4c03509339a4a7487c366bc3827bc47"}}, "title": "Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of Glioma.", "authors": [{"family": "Indira Chandran", "given": "Vineesh", "initials": "V"}, {"family": "Welinder", "given": "Charlotte", "initials": "C", "orcid": "0000-0001-9626-0576", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5715a70ce8df4a5d84ad88709791772a.json"}}, {"family": "M\u00e5nsson", "given": "Ann-Sofie", "initials": "AS"}, {"family": "Offer", "given": "Svenja", "initials": "S"}, {"family": "Freyhult", "given": "Eva", "initials": "E", "orcid": "0000-0003-0226-1047", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/13486bb11790445fa37f592a42e6a7ed.json"}}, {"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Lund", "given": "Sigrid M", "initials": "SM"}, {"family": "Pedersen", "given": "Shona", "initials": "S", "orcid": "0000-0001-6636-0293", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/daa11d544a2545b0aa2a7ec6083fc079.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J"}, {"family": "Marko-Varga", "given": "Gyorgy", "initials": "G"}, {"family": "Johansson", "given": "Maria C", "initials": "MC"}, {"family": "Englund", "given": "Elisabet", "initials": "E", "orcid": "0000-0002-2708-2443", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/8d415783747141269a6d9a843afc861d.json"}}, {"family": "Sundgren", "given": "Pia C", "initials": "PC", "orcid": "0000-0001-9237-1236", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e580179d7d74958a5c1d509e581ddcc.json"}}, {"family": "Belting", "given": "Mattias", "initials": "M"}], "type": "journal article", "published": "2019-05-15", "journal": {"title": "Clin. Cancer Res.", "issn": "1557-3265", "volume": "25", "issue": "10", "pages": "3115-3127", "issn-l": "1078-0432"}, "abstract": "Liquid biopsy has great potential to improve the management of brain tumor patients at high risk of surgery-associated complications. Here, the aim was to explore plasma extracellular vesicle (plEV) immunoprofiling as a tool for noninvasive diagnosis of glioma.\n\nPlEV isolation and analysis were optimized using advanced mass spectrometry, nanoparticle tracking analysis, and electron microscopy. We then established a new procedure that combines size exclusion chromatography isolation and proximity extension assay-based ultrasensitive immunoprofiling of plEV proteins that was applied on a well-defined glioma study cohort (n = 82).\n\nAmong potential candidates, we for the first time identify syndecan-1 (SDC1) as a plEV constituent that can discriminate between high-grade glioblastoma multiforme (GBM, WHO grade IV) and low-grade glioma [LGG, WHO grade II; area under the ROC curve (AUC): 0.81; sensitivity: 71%; specificity: 91%]. These findings were independently validated by ELISA. Tumor SDC1 mRNA expression similarly discriminated between GBM and LGG in an independent glioma patient population from The Cancer Genome Atlas cohort (AUC: 0.91; sensitivity: 79%; specificity: 91%). In experimental studies with GBM cells, we show that SDC1 is efficiently sorted to secreted EVs. Importantly, we found strong support of plEVSDC1 originating from GBM tumors, as plEVSDC1 correlated with SDC1 protein expression in matched patient tumors, and plEVSDC1 was decreased postoperatively depending on the extent of surgery.\n\nOur studies support the concept of circulating plEVs as a tool for noninvasive diagnosis and monitoring of gliomas and should move this field closer to the goal of improving the management of cancer patients.", "doi": "10.1158/1078-0432.CCR-18-2946", "pmid": "30679164", "labels": [], "xrefs": [{"db": "pii", "key": "1078-0432.CCR-18-2946"}], "notes": [], "created": "2026-08-20T12:12:08.365Z", "modified": "2026-08-20T12:12:08.608Z"}, {"entity": "publication", "iuid": "b86e41f154a749929f8a316ac280a772", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b86e41f154a749929f8a316ac280a772.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b86e41f154a749929f8a316ac280a772"}}, "title": "In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta.", "authors": [{"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Sandberg", "given": "AnnSofi", "initials": "A"}, {"family": "Zhu", "given": "Yafeng", "initials": "Y"}, {"family": "Boekel", "given": "Jorrit", "initials": "J"}, {"family": "Tamburro", "given": "Davide", "initials": "D"}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}, {"family": "Bj\u00f6rk", "given": "Albin", "initials": "A"}, {"family": "Wahren-Herlenius", "given": "Marie", "initials": "M"}, {"family": "\u00c5mark", "given": "Hanna", "initials": "H"}, {"family": "\u00d6stenson", "given": "Claes-G\u00f6ran", "initials": "CG"}, {"family": "Westgren", "given": "Magnus", "initials": "M"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/561efcf32e2648c8a10fee692fc4e908.json"}}], "type": "journal article", "published": "2019-04-08", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "8", "issn-l": "2050-084X"}, "abstract": "Here, we present a method for in-depth human plasma proteome analysis based on high-resolution isoelectric focusing HiRIEF LC-MS/MS, demonstrating high proteome coverage, reproducibility and the potential for liquid biopsy protein profiling. By integrating genomic sequence information to the MS-based plasma proteome analysis, we enable detection of single amino acid variants and for the first time demonstrate transfer of multiple protein variants between mother and fetus across the placenta. We further show that our method has the ability to detect both low abundance tissue-annotated proteins and phosphorylated proteins in plasma, as well as quantitate differences in plasma proteomes between the mother and the newborn as well as changes related to pregnancy.", "doi": "10.7554/eLife.41608", "pmid": "30958262", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6519984"}, {"db": "pii", "key": "41608"}], "notes": [], "created": "2026-08-20T13:52:36.748Z", "modified": "2026-08-20T13:52:36.781Z"}, {"entity": "publication", "iuid": "62946e6fa6ab419a87eba4df4be913fd", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/62946e6fa6ab419a87eba4df4be913fd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/62946e6fa6ab419a87eba4df4be913fd"}}, "title": "Author response: In-depth human plasma proteome analysis captures tissue proteins and transfer of protein variants across the placenta", "authors": [{"family": "Pernemalm", "given": "Maria", "initials": "M", "orcid": "0000-0003-4624-031X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c34e0806a3d142019ae8e16de8d720ef.json"}}, {"family": "Sandberg", "given": "AnnSofi", "initials": "A"}, {"family": "Zhu", "given": "Yafeng", "initials": "Y"}, {"family": "Boekel", "given": "Jorrit", "initials": "J"}, {"family": "Tamburro", "given": "Davide", "initials": "D"}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/022e140799b6466ea123319c3d70c76e.json"}}, {"family": "Bj\u00f6rk", "given": "Albin", "initials": "A"}, {"family": "Wahren-Herlenius", "given": "Marie", "initials": "M"}, {"family": "\u00c5mark", "given": "Hanna", "initials": "H"}, {"family": "\u00d6stenson", "given": "Claes G\u00f6ran", "initials": "CG"}, {"family": "Westgren", "given": "Magnus", "initials": "M"}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/561efcf32e2648c8a10fee692fc4e908.json"}}], "type": "peer-review", "published": "2019-02-13", "journal": {"issn-l": null}, "abstract": null, "doi": "10.7554/elife.41608.041", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:52:38.755Z", "modified": "2026-08-20T13:52:38.804Z"}]}