{"entity": "researcher", "timestamp": "2026-08-20T21:24:33.056Z", "family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S", "orcid": "0000-0003-1848-910X", "affiliations": ["Department of Protein Science, KTH Royal Institute of Technology, SciLifeLab, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603"}}, "publications": [{"entity": "publication", "iuid": "c10778938ba2454780c4c85780ef532f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c10778938ba2454780c4c85780ef532f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c10778938ba2454780c4c85780ef532f"}}, "title": "Biological signatures in the Alzheimer's continuum discriminate between diagnosis-related and -unrelated associations to ATN categories.", "authors": [{"family": "Alanko", "given": "Vilma", "initials": "V", "orcid": "0000-0001-9918-9429", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/301242104e7f4d828e1cc2ed4888189c.json"}}, {"family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S", "orcid": "0000-0003-1848-910X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}}, {"family": "Hall", "given": "Anette", "initials": "A"}, {"family": "Hagman", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0003-0238-8646", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/36c280ff51724928b60acdb6f5afed7e.json"}}, {"family": "Mohanty", "given": "Rosaleena", "initials": "R", "orcid": "0000-0001-6499-1251", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/486c11275615496fb8a5f227b2530fde.json"}}, {"family": "Westman", "given": "Eric", "initials": "E", "orcid": "0000-0002-3115-2977", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb9d62f202aa4effa0050bdb34baa9cf.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3db33fa3edb94febb8be7927a16838d0.json"}}, {"family": "Kivipelto", "given": "Miia", "initials": "M", "orcid": "0000-0003-0992-3875", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/26d2d37560514c5b8883e99602a0d5fb.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ccc9581865849c285b687a4670bbf2b.json"}}, {"family": "Matton", "given": "Anna", "initials": "A", "orcid": "0000-0002-7819-0495", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7bacdcd5bca14a2b8437ad3893ff5644.json"}}], "type": "journal article", "published": "2025-02-21", "journal": {"title": "Brain Commun", "issn": "2632-1297", "volume": "7", "issue": "2", "pages": "fcaf078", "issn-l": null}, "abstract": "Alzheimer's disease and related dementias have a multifactorial aetiology and heterogeneous biology. The current study aims to identify different biological signatures in a deeply phenotyped memory clinic patient population. In this cross-sectional study, we analysed 49 pre-specified proteins using a multiplex antibody-based suspension bead array in 278 CSF samples from the real-world research database and biobank at the Karolinska University Hospital Memory Clinic, Solna, Sweden. Patients with a clinical diagnosis of subjective cognitive decline (N = 151), mild cognitive impairment (N = 61), Alzheimer's disease (N = 47), or other diagnoses (N = 19; vascular dementias, alcohol-related dementia, unspecified dementias, or other amnesias) were included. Principal component analyses were performed, and resulting principal components (PCs) were tested for associations with clinical variables and Alzheimer's disease biomarkers (CSF biomarkers beta-amyloid 42, beta-amyloid 42/40, phosphorylated tau 181, phosphorylated tau 181/beta-amyloid 42). PC 1 (explaining 52% of the variance between patients) was associated with the clinical Alzheimer's disease CSF biomarkers beta-amyloid 42, phosphorylated tau 181, and total tau but not with Alzheimer's disease-related neurodegeneration imaging markers, cognitive performance, or clinical diagnosis. PC 2 (explaining 9% of the variance) displayed an inflammatory profile with high contributions of chitinase 3 like 1 (CHI3L1) and triggering receptor expressed on myeloid cells 2 (TREM2) and significant correlation to CSF free light chain kappa. In contrast to PC 1, PC 3 (explaining 5% of the variance) showed associations with all the clinical Alzheimer's disease CSF biomarkers, the imaging markers, cognitive impairment and clinical diagnosis. Serpin family A member 3 (SERPINA3), chitinase 1 (CHIT1), and neuronal pentraxin 2 (NPTX2) contributed most to PC 3. PC 4 (explaining 4% of the variance) exhibited an inflammatory profile distinct from PC 2, with the largest contributions from TREM2, leucine-rich alpha-2-glycoprotein 1 (LRG1) and complement C9. The component was associated with peripheral inflammation. We found that CSF protein profiles in a memory clinic cohort reflect molecular differences across diagnostic groups. Our results emphasize that real-world memory clinic patients can have different ongoing biological processes despite receiving the same diagnosis. In the future, this information could be utilized to identify patient endotypes and uncover precision biomarkers and novel therapeutic targets.", "doi": "10.1093/braincomms/fcaf078", "pmid": "40046342", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11881062"}, {"db": "pii", "key": "fcaf078"}], "notes": [], "created": "2026-08-20T09:40:34.325Z", "modified": "2026-08-20T09:40:34.605Z"}, {"entity": "publication", "iuid": "b4162b0e192e47f185b537a36028e435", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/b4162b0e192e47f185b537a36028e435.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/b4162b0e192e47f185b537a36028e435"}}, "title": "Cerebrospinal fluid levels of NfM in relation to NfL and pNfH as prognostic markers in amyotrophic lateral sclerosis.", "authors": [{"family": "Olofsson", "given": "Jennie", "initials": "J", "orcid": "0000-0002-8593-9089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c0dc7102e4f24b2a9d3f1251fe80f569.json"}}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S", "orcid": "0000-0003-2910-4754", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/552414173f564ce4b813e107812f446d.json"}}, {"family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S", "orcid": "0000-0003-1848-910X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}}, {"family": "Kl\u00e4ppe", "given": "Ulf", "initials": "U", "orcid": "0000-0001-5832-038X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3939bb5c873241299ce82d2aec37457a.json"}}, {"family": "\u00d6ijerstedt", "given": "Linn", "initials": "L", "orcid": "0000-0003-0635-6377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3d1c8d008d08491b9e40516ce0c5806a.json"}}, {"family": "Zetterberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3930-4354", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/18365a2ea4ec4ca2baeb657cd1c8bd8f.json"}}, {"family": "Blennow", "given": "Kaj", "initials": "K", "orcid": "0000-0002-1890-4193", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/57f920b41aab42f0a43a32bbe34035b2.json"}}, {"family": "Ingre", "given": "Caroline", "initials": "C", "orcid": "0000-0001-5327-7204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e6a44fc40ff4b38a3b1da5cf2e8661e.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3db33fa3edb94febb8be7927a16838d0.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ccc9581865849c285b687a4670bbf2b.json"}}], "type": "journal article", "published": "2025-02-00", "journal": {"title": "Amyotroph Lateral Scler Frontotemporal Degener", "issn": "2167-9223", "volume": "26", "issue": "1-2", "pages": "113-123", "issn-l": null}, "abstract": "To evaluate the prognostic potential of neurofilament medium chain (NfM) in CSF from patients with ALS and explore its relationship with the extensively studied neurofilament light chain (NfL) and phosphorylated heavy chain (pNfH).\n\nCSF levels of NfL, NfM, and pNfH were analyzed in 235 samples from patients with ALS, ALS mimics, and healthy controls in a well-characterized cohort from Karolinska ALS Clinical Research Center in Stockholm, Sweden. NfM levels were analyzed using an antibody-based suspension bead-array and NfL and pNfH levels were measured using ELISA. Clinical data, including ALS Revised Functional Rating Scale (ALSFRS-R), and survival outcomes were utilized for disease progression estimations.\n\nIncreased NfM levels were observed in patients with ALS compared with mimics and healthy controls. Similarly, higher NfM levels were found in fast compared with slow progressing patients for baseline and longitudinal progression when evaluating both total and subscores of ALSFRS-R. These findings were consistent with the results observed for NfL and pNfH. All three proteins, used individually as well as in combination, showed comparable performance when classifying fast vs slow progressing patients (AUCs 0.78-0.85). For all neurofilaments, higher survival probability was observed for patients with low CSF levels.\n\nBased on this cross-sectional study, the prognostic value provided by NfM aligns with the more established markers, NfL and pNfH. Additional investigations with independent cohorts and longitudinal studies are needed to further assess the potential added value of NfM.", "doi": "10.1080/21678421.2024.2428930", "pmid": "39575564", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T09:37:40.836Z", "modified": "2026-08-20T09:37:41.085Z"}, {"entity": "publication", "iuid": "bea27aac30214f489afdb3c8ba1ddcd2", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bea27aac30214f489afdb3c8ba1ddcd2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bea27aac30214f489afdb3c8ba1ddcd2"}}, "title": "Cerebrospinal fluid levels of NfM in relation to NfL and pNfH as prognostic markers in amyotrophic lateral sclerosis", "authors": [{"family": "Olofsson", "given": "Jennie", "initials": "J", "orcid": "0000-0002-8593-9089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c0dc7102e4f24b2a9d3f1251fe80f569.json"}}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S", "orcid": "0000-0003-2910-4754", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/552414173f564ce4b813e107812f446d.json"}}, {"family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S", "orcid": "0000-0003-1848-910X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}}, {"family": "Kl\u00e4ppe", "given": "Ulf", "initials": "U", "orcid": "0000-0001-5832-038X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3939bb5c873241299ce82d2aec37457a.json"}}, {"family": "\u00d6ijerstedt", "given": "Linn", "initials": "L", "orcid": "0000-0003-0635-6377", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3d1c8d008d08491b9e40516ce0c5806a.json"}}, {"family": "Zetterberg", "given": "Henrik", "initials": "H", "orcid": "0000-0003-3930-4354", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/18365a2ea4ec4ca2baeb657cd1c8bd8f.json"}}, {"family": "Blennow", "given": "Kaj", "initials": "K", "orcid": "0000-0002-1890-4193", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/57f920b41aab42f0a43a32bbe34035b2.json"}}, {"family": "Ingre", "given": "Caroline", "initials": "C", "orcid": "0000-0001-5327-7204", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6e6a44fc40ff4b38a3b1da5cf2e8661e.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3db33fa3edb94febb8be7927a16838d0.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ccc9581865849c285b687a4670bbf2b.json"}}], "type": "posted-content", "published": "2024-07-10", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.07.09.24310135", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:55:36.267Z", "modified": "2026-08-20T10:55:36.374Z"}, {"entity": "publication", "iuid": "7e607955637a4b4aafa78db842ae0bbb", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7e607955637a4b4aafa78db842ae0bbb.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7e607955637a4b4aafa78db842ae0bbb"}}, "title": "Addressing inter-individual variability in CSF levels of brain-derived proteins across neurodegenerative diseases", "authors": [{"family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S"}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S", "orcid": "0000-0003-2910-4754", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/552414173f564ce4b813e107812f446d.json"}}, {"family": "Olofsson", "given": "Jennie", "initials": "J", "orcid": "0000-0002-8593-9089", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c0dc7102e4f24b2a9d3f1251fe80f569.json"}}, {"family": "de San Jos\u00e9", "given": "Nerea G\u00f3mez", "initials": "NG"}, {"family": "Anderl-Straub", "given": "Sarah", "initials": "S", "orcid": "0000-0003-1848-910X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}}, {"family": "Diehl-Schmid", "given": "Janine", "initials": "J"}, {"family": "Fassbender", "given": "Klaus", "initials": "K"}, {"family": "Fliessbach", "given": "Klaus", "initials": "K"}, {"family": "Jahn", "given": "Holger", "initials": "H"}, {"family": "Kornhuber", "given": "Johannes", "initials": "J"}, {"family": "Landwehrmeyer", "given": "G Bernhard", "initials": "GB"}, {"family": "Lauer", "given": "Martin", "initials": "M"}, {"family": "Levin", "given": "Johannes", "initials": "J"}, {"family": "Ludolph", "given": "Albert C", "initials": "AC"}, {"family": "Prudlo", "given": "Johannes", "initials": "J"}, {"family": "Schneider", "given": "Anja", "initials": "A"}, {"family": "Schroeter", "given": "Matthias L", "initials": "ML"}, {"family": "Wiltfang", "given": "Jens", "initials": "J"}, {"family": "Steinacker", "given": "Petra", "initials": "P"}, {"family": "Otto", "given": "Markus", "initials": "M", "orcid": "0000-0003-4273-4267", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/5bee470b0e4e45a2b553d849d8299e2f.json"}}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3db33fa3edb94febb8be7927a16838d0.json"}}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A", "orcid": "0000-0002-0056-1313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ccc9581865849c285b687a4670bbf2b.json"}}], "type": "posted-content", "published": "2024-05-27", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2024.05.27.24307739", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T10:54:55.969Z", "modified": "2026-08-20T10:54:56.092Z"}, {"entity": "publication", "iuid": "715deb5c6c37494fa0f6fa41872a00a9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/715deb5c6c37494fa0f6fa41872a00a9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/715deb5c6c37494fa0f6fa41872a00a9"}}, "title": "CSF protein ratios with enhanced potential to reflect Alzheimer's disease pathology and neurodegeneration.", "authors": [{"family": "Mravinacov\u00e1", "given": "S\u00e1ra", "initials": "S", "orcid": "0000-0003-1848-910X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c1e48837666642b18a977bb8a8c39603.json"}}, {"family": "Alanko", "given": "Vilma", "initials": "V"}, {"family": "Bergstr\u00f6m", "given": "Sofia", "initials": "S"}, {"family": "Bridel", "given": "Claire", "initials": "C"}, {"family": "Pijnenburg", "given": "Yolande", "initials": "Y"}, {"family": "Hagman", "given": "G\u00f6ran", "initials": "G"}, {"family": "Kivipelto", "given": "Miia", "initials": "M"}, {"family": "Teunissen", "given": "Charlotte", "initials": "C"}, {"family": "Nilsson", "given": "Peter", "initials": "P"}, {"family": "Matton", "given": "Anna", "initials": "A"}, {"family": "M\u00e5nberg", "given": "Anna", "initials": "A"}], "type": "journal article", "published": "2024-02-13", "journal": {"title": "Mol Neurodegener", "issn": "1750-1326", "volume": "19", "issue": "1", "pages": "15", "issn-l": "1750-1326"}, "abstract": "Amyloid and tau aggregates are considered to cause neurodegeneration and consequently cognitive decline in individuals with Alzheimer's disease (AD). Here, we explore the potential of cerebrospinal fluid (CSF) proteins to reflect AD pathology and cognitive decline, aiming to identify potential biomarkers for monitoring outcomes of disease-modifying therapies targeting these aggregates.\n\nWe used a multiplex antibody-based suspension bead array to measure the levels of 49 proteins in CSF from the Swedish GEDOC memory clinic cohort at the Karolinska University Hospital. The cohort comprised 148 amyloid- and tau-negative individuals (A-T-) and 65 amyloid- and tau-positive individuals (A+T+). An independent sample set of 26 A-T- and 26 A+T+ individuals from the Amsterdam Dementia Cohort was used for validation. The measured proteins were clustered based on their correlation to CSF amyloid beta peptides, tau and NfL levels. Further, we used support vector machine modelling to identify protein pairs, matched based on their cluster origin, that reflect AD pathology and cognitive decline with improved performance compared to single proteins.\n\nThe protein-clustering revealed 11 proteins strongly correlated to t-tau and p-tau (tau-associated group), including mainly synaptic proteins previously found elevated in AD such as NRGN, GAP43 and SNCB. Another 16 proteins showed predominant correlation with A\u03b242 (amyloid-associated group), including PTPRN2, NCAN and CHL1. Support vector machine modelling revealed that proteins from the two groups combined in pairs discriminated A-T- from A+T+ individuals with higher accuracy compared to single proteins, as well as compared to protein pairs composed of proteins originating from the same group. Moreover, combining the proteins from different groups in ratios (tau-associated protein/amyloid-associated protein) significantly increased their correlation to cognitive decline measured with cognitive scores. The results were validated in an independent cohort.\n\nCombining brain-derived proteins in pairs largely enhanced their capacity to discriminate between AD pathology-affected and unaffected individuals and increased their correlation to cognitive decline, potentially due to adjustment of inter-individual variability. With these results, we highlight the potential of protein pairs to monitor neurodegeneration and thereby possibly the efficacy of AD disease-modifying therapies.", "doi": "10.1186/s13024-024-00705-z", "pmid": "38350954", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10863228"}, {"db": "pii", "key": "10.1186/s13024-024-00705-z"}], "notes": [], "created": "2026-08-20T12:21:17.479Z", "modified": "2026-08-20T12:21:17.515Z"}]}