{"entity": "researcher", "timestamp": "2026-10-10T11:33:27.917Z", "family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "affiliations": ["New York Genome Center, New York, NY 10013, USA."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0"}}, "publications": [{"entity": "publication", "iuid": "0cdd265652aa4f389e3bace451468221", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/0cdd265652aa4f389e3bace451468221.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/0cdd265652aa4f389e3bace451468221"}}, "title": "Nonlinear transcriptional responses to gradual modulation of transcription factor dosage.", "authors": [{"family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}}, {"family": "Minaeva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-7028-1264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c146bcfb925444b78cab53b61cf912e2.json"}}, {"family": "Morris", "given": "John A", "initials": "JA", "orcid": "0000-0003-2769-8202", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61b4dd70c06748a2abd7aa2a73200b40.json"}}, {"family": "Ghatan", "given": "Samuel", "initials": "S"}, {"family": "Ziosi", "given": "Marcello", "initials": "M", "orcid": "0000-0001-5128-6158", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b75170862b824a698666eb8d3938d581.json"}}, {"family": "Sanjana", "given": "Neville E", "initials": "NE", "orcid": "0000-0002-1504-0027", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fee65a1527cc49e2942d946fba4ded65.json"}}, {"family": "Lappalainen", "given": "Tuuli", "initials": "T", "orcid": "0000-0002-7746-8109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8401dbf3ffb4f35bcc8e16f4a04cd7c.json"}}], "type": "journal article", "published": "2026-01-14", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "13", "issn-l": "2050-084X"}, "abstract": "Genomic loci associated with common traits and diseases are typically non-coding and likely impact gene expression, sometimes coinciding with rare loss-of-function variants in the target gene. However, our understanding of how gradual changes in gene dosage affect molecular, cellular, and organismal traits is currently limited. To address this gap, we induced gradual changes in gene expression of four genes using CRISPR activation and inactivation in human-derived K562 cells. Downstream transcriptional consequences of dosage modulation of three master trans-regulators associated with blood cell traits (GFI1B, NFE2, and MYB) were examined using targeted single-cell multimodal sequencing. We showed that guide tiling around the TSS is the most effective way to modulate cis gene expression across a wide range of fold changes, with further effects from chromatin accessibility and histone marks that differ between the inhibition and activation systems. Our single-cell data allowed us to precisely detect subtle to large gene expression changes in dozens of trans genes, revealing that many responses to dosage changes of these three TFs are nonlinear, including non-monotonic behaviours, even when constraining the fold changes of the master regulators to a copy number gain or loss. We found that the dosage properties are linked to gene constraint and that some of these nonlinear responses are enriched for disease and GWAS genes. Overall, our study provides a straightforward and scalable method to precisely modulate gene expression and gain insights into its downstream consequences at high resolution.", "doi": "10.7554/eLife.100555", "pmid": "41532405", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC12803512"}, {"db": "pii", "key": "100555"}, {"db": "GEO", "key": "GSE257547"}], "notes": [], "created": "2026-08-20T13:50:49.996Z", "modified": "2026-09-23T14:12:02.213Z"}, {"entity": "publication", "iuid": "2ab4b223c10c4511b4e636c080ec13cc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2ab4b223c10c4511b4e636c080ec13cc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2ab4b223c10c4511b4e636c080ec13cc"}}, "title": "Nonlinear transcriptional responses to gradual modulation of transcription factor dosage", "authors": [{"family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}}, {"family": "Minaeva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-7028-1264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c146bcfb925444b78cab53b61cf912e2.json"}}, {"family": "Morris", "given": "John A", "initials": "JA", "orcid": "0000-0003-2769-8202", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61b4dd70c06748a2abd7aa2a73200b40.json"}}, {"family": "Ghatan", "given": "Samuel", "initials": "S"}, {"family": "Ziosi", "given": "Marcello", "initials": "M", "orcid": "0000-0001-5128-6158", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b75170862b824a698666eb8d3938d581.json"}}, {"family": "Sanjana", "given": "Neville E", "initials": "NE", "orcid": "0000-0002-1504-0027", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fee65a1527cc49e2942d946fba4ded65.json"}}, {"family": "Lappalainen", "given": "Tuuli", "initials": "T", "orcid": "0000-0002-7746-8109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8401dbf3ffb4f35bcc8e16f4a04cd7c.json"}}], "type": "posted-content", "published": "2025-11-25", "journal": {"issn-l": null}, "abstract": null, "doi": "10.7554/elife.100555.2", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:50:54.071Z", "modified": "2026-09-23T14:54:37.567Z"}, {"entity": "publication", "iuid": "7b3e9f824f464167aec23b08c33edda4", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/7b3e9f824f464167aec23b08c33edda4.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/7b3e9f824f464167aec23b08c33edda4"}}, "title": "Nonlinear transcriptional responses to gradual modulation of transcription factor dosage.", "authors": [{"family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}}, {"family": "Minaeva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-7028-1264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c146bcfb925444b78cab53b61cf912e2.json"}}, {"family": "Morris", "given": "John A", "initials": "JA", "orcid": "0000-0003-2769-8202", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61b4dd70c06748a2abd7aa2a73200b40.json"}}, {"family": "Ghatan", "given": "Samuel", "initials": "S"}, {"family": "Ziosi", "given": "Marcello", "initials": "M", "orcid": "0000-0001-5128-6158", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b75170862b824a698666eb8d3938d581.json"}}, {"family": "Sanjana", "given": "Neville E", "initials": "NE", "orcid": "0000-0002-1504-0027", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fee65a1527cc49e2942d946fba4ded65.json"}}, {"family": "Lappalainen", "given": "Tuuli", "initials": "T", "orcid": "0000-0002-7746-8109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8401dbf3ffb4f35bcc8e16f4a04cd7c.json"}}], "type": "journal article", "published": "2025-08-07", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null}, "abstract": "Genomic loci associated with common traits and diseases are typically non-coding and likely impact gene expression, sometimes coinciding with rare loss-of-function variants in the target gene. However, our understanding of how gradual changes in gene dosage affect molecular, cellular, and organismal traits is currently limited. To address this gap, we induced gradual changes in gene expression of four genes using CRISPR activation and inactivation. Downstream transcriptional consequences of dosage modulation of three master trans-regulators associated with blood cell traits (GFI1B, NFE2, and MYB) were examined using targeted single-cell multimodal sequencing. We showed that guide tiling around the TSS is the most effective way to modulate cis gene expression across a wide range of fold-changes, with further effects from chromatin accessibility and histone marks that differ between the inhibition and activation systems. Our single-cell data allowed us to precisely detect subtle to large gene expression changes in dozens of trans genes, revealing that many responses to dosage changes of these three TFs are nonlinear, including non-monotonic behaviours, even when constraining the fold-changes of the master regulators to a copy number gain or loss. We found that the dosage properties are linked to gene constraint and that some of these nonlinear responses are enriched for disease and GWAS genes. Overall, our study provides a straightforward and scalable method to precisely modulate gene expression and gain insights into its downstream consequences at high resolution.", "doi": "10.1101/2024.03.01.582837", "pmid": "38464330", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10925300"}, {"db": "pii", "key": "2024.03.01.582837"}], "notes": [], "created": "2026-08-20T10:52:31.753Z", "modified": "2026-09-23T13:46:33.035Z"}, {"entity": "publication", "iuid": "2c08842d2ad0418a8807a686f340dab1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/2c08842d2ad0418a8807a686f340dab1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/2c08842d2ad0418a8807a686f340dab1"}}, "title": "Non-linear transcriptional responses to gradual modulation of transcription factor dosage", "authors": [{"family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}}, {"family": "Minaeva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-7028-1264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c146bcfb925444b78cab53b61cf912e2.json"}}, {"family": "Morris", "given": "John A", "initials": "JA", "orcid": "0000-0003-2769-8202", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/61b4dd70c06748a2abd7aa2a73200b40.json"}}, {"family": "Ghatan", "given": "Sam", "initials": "S"}, {"family": "Ziosi", "given": "Marcello", "initials": "M", "orcid": "0000-0001-5128-6158", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b75170862b824a698666eb8d3938d581.json"}}, {"family": "Sanjana", "given": "Neville E", "initials": "NE", "orcid": "0000-0002-1504-0027", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/fee65a1527cc49e2942d946fba4ded65.json"}}, {"family": "Lappalainen", "given": "Tuuli", "initials": "T", "orcid": "0000-0002-7746-8109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8401dbf3ffb4f35bcc8e16f4a04cd7c.json"}}], "type": "posted-content", "published": "2024-12-17", "journal": {"issn-l": null}, "abstract": null, "doi": "10.7554/elife.100555.1", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T13:50:52.021Z", "modified": "2026-09-23T14:22:17.283Z"}, {"entity": "publication", "iuid": "c2ab2f0a381e46688fd64c494a272cb9", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/c2ab2f0a381e46688fd64c494a272cb9.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/c2ab2f0a381e46688fd64c494a272cb9"}}, "title": "Specifying cellular context of transcription factor regulons for exploring context-specific gene regulation programs.", "authors": [{"family": "Minaeva", "given": "Mariia", "initials": "M", "orcid": "0000-0001-7028-1264", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c146bcfb925444b78cab53b61cf912e2.json"}}, {"family": "Domingo", "given": "J\u00falia", "initials": "J", "orcid": "0000-0001-6359-2785", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c038c148261945cc8b4c0bd09c99b4e0.json"}}, {"family": "Rentzsch", "given": "Philipp", "initials": "P", "orcid": "0000-0002-0413-7974", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/768e0f165868444baa175d4958bec15c.json"}}, {"family": "Lappalainen", "given": "Tuuli", "initials": "T", "orcid": "0000-0002-7746-8109", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e8401dbf3ffb4f35bcc8e16f4a04cd7c.json"}}], "type": "preprint", "published": "2024-01-01", "journal": {"title": "bioRxiv", "issn": "2692-8205", "issn-l": null}, "abstract": "Understanding the role of transcription and transcription factors in cellular identity and disease, such as cancer and autoimmunity, is essential. However, comprehensive data resources for cell line-specific transcription factor-to-target gene annotations are currently limited. To address this, we developed a straightforward method to define regulons that capture the cell-specific aspects of TF binding and transcript expression levels. By integrating cellular transcriptome and transcription factor binding data, we generated regulons for four common cell lines comprising both proximal and distal cell line-specific regulatory events. Through systematic benchmarking involving transcription factor knockout experiments, we demonstrated performance on par with state-of-the-art methods, with our method being easily applicable to other cell types of interest. We present case studies using three cancer single-cell datasets to showcase the utility of these cell-type-specific regulons in exploring transcriptional dysregulation. In summary, this study provides a valuable tool and a resource for systematically exploring cell line-specific transcriptional regulations, emphasizing the utility of network analysis in deciphering disease mechanisms.", "doi": "10.1101/2023.12.31.573765", "pmid": "38260658", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10802353"}, {"db": "pii", "key": "2023.12.31.573765"}], "notes": [], "created": "2026-08-20T10:51:23.163Z", "modified": "2026-09-23T14:22:01.827Z"}]}