{"entity": "researcher", "timestamp": "2026-08-20T20:42:32.429Z", "family": "Bucher", "given": "Maximilian", "initials": "M", "orcid": "0000-0001-7896-473X", "affiliations": ["SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.", "Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, USA.", "Institut f\u00fcr Optik und Atomare Physik, Technische Universit\u00e4t Berlin, Hardenbergstra\u00dfe 36, Berlin 10623, Germany."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/bff3b3f3e7504b5fba4e5ead46fc22be.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/bff3b3f3e7504b5fba4e5ead46fc22be"}}, "publications": [{"entity": "publication", "iuid": "e6fb98199d6545378b4d0dd55ff791e1", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e6fb98199d6545378b4d0dd55ff791e1.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e6fb98199d6545378b4d0dd55ff791e1"}}, "title": "Electrospray sample injection for single-particle imaging with x-ray lasers.", "authors": [{"family": "Bielecki", "given": "Johan", "initials": "J", "orcid": "0000-0002-3012-603X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/cb981ea4fae1468fbf068f2b4b509c75.json"}}, {"family": "Hantke", "given": "Max F", "initials": "MF", "orcid": "0000-0003-1251-0465", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/803e4afe61af49fc8a37f3b5b0522ea7.json"}}, {"family": "Daurer", "given": "Benedikt J", "initials": "BJ", "orcid": "0000-0002-1887-7551", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/22e1c4b4b7fc427f9069efa4b1486d47.json"}}, {"family": "Reddy", "given": "Hemanth K N", "initials": "HKN"}, {"family": "Hasse", "given": "Dirk", "initials": "D", "orcid": "0000-0001-8710-327X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/4395deedd98b4a82867e6034fc11ad79.json"}}, {"family": "Larsson", "given": "Daniel S D", "initials": "DSD", "orcid": "0000-0001-7683-6419", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b7f10a849faa444ab45f565e86abac5a.json"}}, {"family": "Gunn", "given": "Laura H", "initials": "LH", "orcid": "0000-0003-2072-0884", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/35fd1150a0f64d7db5b81ace81bb7f4f.json"}}, {"family": "Svenda", "given": "Martin", "initials": "M"}, {"family": "Munke", "given": "Anna", "initials": "A", "orcid": "0000-0002-5510-2245", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e3b83fcda1004315a30e61c7c2c678f7.json"}}, {"family": "Sellberg", "given": "Jonas A", "initials": "JA", "orcid": "0000-0003-2793-5052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/86c7efd806b5436296298d6243778574.json"}}, {"family": "Flueckiger", "given": "Leonie", "initials": "L"}, {"family": "Pietrini", "given": "Alberto", "initials": "A", "orcid": "0000-0002-1171-6683", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c36ada5fa2c64f85ade5c5ff616d5c6d.json"}}, {"family": "Nettelblad", "given": "Carl", "initials": "C", "orcid": "0000-0003-0458-6902", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/da4567d9202441e29412c827729e7134.json"}}, {"family": "Lundholm", "given": "Ida", "initials": "I"}, {"family": "Carlsson", "given": "Gunilla", "initials": "G", "orcid": "0000-0002-7565-0177", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7409d72a83104ceebf22dfe6e19e756b.json"}}, {"family": "Okamoto", "given": "Kenta", "initials": "K"}, {"family": "Timneanu", "given": "Nicusor", "initials": "N", "orcid": "0000-0001-7328-0400", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c2a9165954da4b14b334d1d7151efb9e.json"}}, {"family": "Westphal", "given": "Daniel", "initials": "D"}, {"family": "Kulyk", "given": "Olena", "initials": "O"}, {"family": "Higashiura", "given": "Akifumi", "initials": "A", "orcid": "0000-0001-8448-9349", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/848a654049a84a81b6d88bb2603fb82b.json"}}, {"family": "van der Schot", "given": "Gijs", "initials": "G", "orcid": "0000-0002-6128-4196", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/79d5acb7b94846aa9819e92a99dae0b9.json"}}, {"family": "Loh", "given": "Ne-Te Duane", "initials": "ND", "orcid": "0000-0002-8886-510X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1051c98d2c724698bcc25803a0404457.json"}}, {"family": "Wysong", "given": "Taylor E", "initials": "TE"}, {"family": "Bostedt", "given": "Christoph", "initials": "C"}, {"family": "Gorkhover", "given": "Tais", "initials": "T"}, {"family": "Iwan", "given": "Bianca", "initials": "B", "orcid": "0000-0003-4352-013X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2510f70c398449f8973ce5f145f6ac8b.json"}}, {"family": "Seibert", "given": "M Marvin", "initials": "MM"}, {"family": "Osipov", "given": "Timur", "initials": "T"}, {"family": "Walter", "given": "Peter", "initials": "P"}, {"family": "Hart", "given": "Philip", "initials": "P", "orcid": "0000-0002-4569-3926", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b247ceeca3644ec39cc8bcfe33180591.json"}}, {"family": "Bucher", "given": "Maximilian", "initials": "M", "orcid": "0000-0001-7896-473X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bff3b3f3e7504b5fba4e5ead46fc22be.json"}}, {"family": "Ulmer", "given": "Anatoli", "initials": "A"}, {"family": "Ray", "given": "Dipanwita", "initials": "D"}, {"family": "Carini", "given": "Gabriella", "initials": "G"}, {"family": "Ferguson", "given": "Ken R", "initials": "KR"}, {"family": "Andersson", "given": "Inger", "initials": "I", "orcid": "0000-0002-2729-0787", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d190a3ec37394343b3b27fab0f2ed5bc.json"}}, {"family": "Andreasson", "given": "Jakob", "initials": "J", "orcid": "0000-0002-3202-2330", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1d15aba5354347b283b16122bef977ca.json"}}, {"family": "Hajdu", "given": "Janos", "initials": "J", "orcid": "0000-0002-3747-2760", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ef8aa893b7854bea83edb8993f453162.json"}}, {"family": "Maia", "given": "Filipe R N C", "initials": "FRNC", "orcid": "0000-0002-2141-438X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a305c750241840a19612dc53e34beacc.json"}}], "type": "journal article", "published": "2019-05-00", "journal": {"title": "Sci Adv", "issn": "2375-2548", "volume": "5", "issue": "5", "pages": "eaav8801", "issn-l": "2375-2548"}, "abstract": "The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.", "doi": "10.1126/sciadv.aav8801", "pmid": "31058226", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC6499549"}, {"db": "pii", "key": "aav8801"}], "notes": [], "created": "2026-08-20T11:58:27.408Z", "modified": "2026-08-20T11:58:28.536Z"}, {"entity": "publication", "iuid": "e6c0d332d61f4640843ff4931598477e", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/e6c0d332d61f4640843ff4931598477e.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/e6c0d332d61f4640843ff4931598477e"}}, "title": "Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source.", "authors": [{"family": "Munke", "given": "Anna", "initials": "A", "orcid": "0000-0002-5510-2245", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e3b83fcda1004315a30e61c7c2c678f7.json"}}, {"family": "Andreasson", "given": "Jakob", "initials": "J"}, {"family": "Aquila", "given": "Andrew", "initials": "A"}, {"family": "Awel", "given": "Salah", "initials": "S"}, {"family": "Ayyer", "given": "Kartik", "initials": "K"}, {"family": "Barty", "given": "Anton", "initials": "A"}, {"family": "Bean", "given": "Richard J", "initials": "RJ"}, {"family": "Berntsen", "given": "Peter", "initials": "P"}, {"family": "Bielecki", "given": "Johan", "initials": "J"}, {"family": "Boutet", "given": "S\u00e9bastien", "initials": "S"}, {"family": "Bucher", "given": "Maximilian", "initials": "M", "orcid": "0000-0001-7896-473X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/bff3b3f3e7504b5fba4e5ead46fc22be.json"}}, {"family": "Chapman", "given": "Henry N", "initials": "HN"}, {"family": "Daurer", "given": "Benedikt J", "initials": "BJ", "orcid": "0000-0002-1887-7551", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/22e1c4b4b7fc427f9069efa4b1486d47.json"}}, {"family": "DeMirci", "given": "Hasan", "initials": "H", "orcid": "0000-0002-9135-5397", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/2e0493a130604cea86ba6e3bc188df8c.json"}}, {"family": "Elser", "given": "Veit", "initials": "V"}, {"family": "Fromme", "given": "Petra", "initials": "P"}, {"family": "Hajdu", "given": "Janos", "initials": "J"}, {"family": "Hantke", "given": "Max F", "initials": "MF"}, {"family": "Higashiura", "given": "Akifumi", "initials": "A"}, {"family": "Hogue", "given": "Brenda G", "initials": "BG"}, {"family": "Hosseinizadeh", "given": "Ahmad", "initials": "A"}, {"family": "Kim", "given": "Yoonhee", "initials": "Y"}, {"family": "Kirian", "given": "Richard A", "initials": "RA"}, {"family": "Reddy", "given": "Hemanth K N", "initials": "HK"}, {"family": "Lan", "given": "Ti-Yen", "initials": "TY"}, {"family": "Larsson", "given": "Daniel S D", "initials": "DS"}, {"family": "Liu", "given": "Haiguang", "initials": "H", "orcid": "0000-0001-7324-6632", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b6fcf493cacb4132b34d2400965a34b4.json"}}, {"family": "Loh", "given": "N Duane", "initials": "ND"}, {"family": "Maia", "given": "Filipe R N C", "initials": "FR", "orcid": "0000-0002-2141-438X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/a305c750241840a19612dc53e34beacc.json"}}, {"family": "Mancuso", "given": "Adrian P", "initials": "AP"}, {"family": "M\u00fchlig", "given": "Kerstin", "initials": "K"}, {"family": "Nakagawa", "given": "Atsushi", "initials": "A", "orcid": "0000-0002-1700-7861", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/d332e49a07cc479abdde79e160b888f9.json"}}, {"family": "Nam", "given": "Daewoong", "initials": "D"}, {"family": "Nelson", "given": "Garrett", "initials": "G"}, {"family": "Nettelblad", "given": "Carl", "initials": "C"}, {"family": "Okamoto", "given": "Kenta", "initials": "K"}, {"family": "Ourmazd", "given": "Abbas", "initials": "A", "orcid": "0000-0001-9946-3889", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/75dd2011254b4b908c484f74284c3388.json"}}, {"family": "Rose", "given": "Max", "initials": "M"}, {"family": "van der Schot", "given": "Gijs", "initials": "G"}, {"family": "Schwander", "given": "Peter", "initials": "P"}, {"family": "Seibert", "given": "M Marvin", "initials": "MM"}, {"family": "Sellberg", "given": "Jonas A", "initials": "JA", "orcid": "0000-0003-2793-5052", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/86c7efd806b5436296298d6243778574.json"}}, {"family": "Sierra", "given": "Raymond G", "initials": "RG"}, {"family": "Song", "given": "Changyong", "initials": "C"}, {"family": "Svenda", "given": "Martin", "initials": "M"}, {"family": "Timneanu", "given": "Nicusor", "initials": "N", "orcid": "0000-0001-7328-0400", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/c2a9165954da4b14b334d1d7151efb9e.json"}}, {"family": "Vartanyants", "given": "Ivan A", "initials": "IA"}, {"family": "Westphal", "given": "Daniel", "initials": "D"}, {"family": "Wiedorn", "given": "Max O", "initials": "MO"}, {"family": "Williams", "given": "Garth J", "initials": "GJ"}, {"family": "Xavier", "given": "Paulraj Lourdu", "initials": "PL"}, {"family": "Yoon", "given": "Chun Hong", "initials": "CH"}, {"family": "Zook", "given": "James", "initials": "J"}], "type": "dataset", "published": "2016-08-01", "journal": {"title": "Sci Data", "issn": "2052-4463", "volume": "3", "pages": "160064", "issn-l": "2052-4463"}, "abstract": "Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 \u03bcm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 \u00c5ngstr\u00f6m were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.", "doi": "10.1038/sdata.2016.64", "pmid": "27478984", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC4968191"}, {"db": "pii", "key": "sdata201664"}, {"db": "figshare", "key": "10.6084/M9.FIGSHARE.C.2342581"}], "notes": [], "created": "2026-08-20T09:27:01.495Z", "modified": "2026-08-20T09:27:01.848Z"}]}