{"entity": "researcher", "timestamp": "2026-09-26T22:59:55.293Z", "family": "Pineau", "given": "Charles", "initials": "C", "orcid": "0000-0002-7461-5433", "affiliations": ["Univ Rennes , Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail), UMR_S 1085 , 35042 Rennes Cedex, France.", "Protim , Univ Rennes , 35042 Rennes Cedex, France."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/be0701af8ec049d4bc78c9fd09751acd.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/be0701af8ec049d4bc78c9fd09751acd"}}, "publications": [{"entity": "publication", "iuid": "77b7103ae19d4f77a604a5c68263aa58", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/77b7103ae19d4f77a604a5c68263aa58.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/77b7103ae19d4f77a604a5c68263aa58"}}, "title": "Cell Type-Specific Expression of Testis Elevated Genes Based on Transcriptomics and Antibody-Based Proteomics.", "authors": [{"family": "Pineau", "given": "Charles", "initials": "C", "orcid": "0000-0002-7461-5433", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be0701af8ec049d4bc78c9fd09751acd.json"}}, {"family": "Hikmet", "given": "Feria", "initials": "F"}, {"family": "Zhang", "given": "Cheng", "initials": "C"}, {"family": "Oksvold", "given": "Per", "initials": "P"}, {"family": "Chen", "given": "Shuqi", "initials": "S"}, {"family": "Fagerberg", "given": "Linn", "initials": "L"}, {"family": "Uhl\u00e9n", "given": "Mathias", "initials": "M"}, {"family": "Lindskog", "given": "Cecilia", "initials": "C", "orcid": "0000-0001-5611-1015", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/21610a810a87459c962d6da3f2ad38ad.json"}}], "type": "journal article", "published": "2019-12-06", "journal": {"title": "J. Proteome Res.", "issn": "1535-3907", "volume": "18", "issue": "12", "pages": "4215-4230", "issn-l": "1535-3893"}, "abstract": "One of the most complex organs in the human body is the testis, where spermatogenesis takes place. This physiological process involves thousands of genes and proteins that are activated and repressed, making testis the organ with the highest number of tissue-specific genes. However, the function of a large proportion of the corresponding proteins remains unknown and testis harbors many missing proteins (MPs), defined as products of protein-coding genes that lack experimental mass spectrometry evidence. Here, an integrated omics approach was used for exploring the cell type-specific protein expression of genes with an elevated expression in testis. By combining genome-wide transcriptomics analysis with immunohistochemistry, more than 500 proteins with distinct testicular protein expression patterns were identified, and these were selected for in-depth characterization of their in situ expression in eight different testicular cell types. The cell type-specific protein expression patterns allowed us to identify six distinct clusters of expression at different stages of spermatogenesis. The analysis highlighted numerous poorly characterized proteins in each of these clusters whose expression overlapped with that of known proteins involved in spermatogenesis, including 85 proteins with an unknown function and 60 proteins that previously have been classified as MPs. Furthermore, we were able to characterize the in situ distribution of several proteins that previously lacked spatial information and cell type-specific expression within the testis. The testis elevated expression levels both at the RNA and protein levels suggest that these proteins are related to testis-specific functions. In summary, the study demonstrates the power of combining genome-wide transcriptomics analysis with antibody-based protein profiling to explore the cell type-specific expression of both well-known proteins and MPs. The analyzed proteins constitute important targets for further testis-specific research in male reproductive disorders.", "doi": "10.1021/acs.jproteome.9b00351", "pmid": "31429579", "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T09:28:04.955Z", "modified": "2026-09-23T09:28:05.008Z"}, {"entity": "publication", "iuid": "f119aa262f664b4c9bf3e7ae2f5af99f", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/f119aa262f664b4c9bf3e7ae2f5af99f.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/f119aa262f664b4c9bf3e7ae2f5af99f"}}, "title": "Validating Missing Proteins in Human Sperm Cells by Targeted Mass-Spectrometry- and Antibody-based Methods.", "authors": [{"family": "Carapito", "given": "Christine", "initials": "C"}, {"family": "Duek", "given": "Paula", "initials": "P"}, {"family": "Macron", "given": "Charlotte", "initials": "C"}, {"family": "Seffals", "given": "Marine", "initials": "M"}, {"family": "Rondel", "given": "Karine", "initials": "K"}, {"family": "Delalande", "given": "Fran\u00e7ois", "initials": "F"}, {"family": "Lindskog", "given": "Cecilia", "initials": "C"}, {"family": "Fr\u00e9our", "given": "Thomas", "initials": "T"}, {"family": "Vandenbrouck", "given": "Yves", "initials": "Y"}, {"family": "Lane", "given": "Lydie", "initials": "L"}, {"family": "Pineau", "given": "Charles", "initials": "C", "orcid": "0000-0002-7461-5433", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/be0701af8ec049d4bc78c9fd09751acd.json"}}], "type": "journal article", "published": "2017-12-01", "journal": {"title": "J. Proteome Res.", "issn": "1535-3907", "volume": "16", "issue": "12", "pages": "4340-4351", "issn-l": "1535-3893"}, "abstract": "The present study is a contribution to the \"neXt50 challenge\", a coordinated effort across C-HPP teams to identify the 50 most tractable missing proteins (MPs) on each chromosome. We report the targeted search of 38 theoretically detectable MPs from chromosomes 2 and 14 in Triton X-100 soluble and insoluble sperm fractions from a total of 15 healthy donors. A targeted mass-spectrometry-based strategy consisting of the development of LC-PRM assays (with heavy labeled synthetic peptides) targeting 92 proteotypic peptides of the 38 selected MPs was used. Out of the 38 selected MPs, 12 were identified with two or more peptides and 3 with one peptide after extensive SDS-PAGE fractionation of the two samples and with overall low-intensity signals. The PRM data are available via ProteomeXchange in PASSEL (PASS01013). Further validation by immunohistochemistry on human testes sections and cytochemistry on sperm smears was performed for eight MPs with antibodies available from the Human Protein Atlas. Deep analysis of human sperm still allows the validation of MPs and therefore contributes to the C-HPP worldwide effort. We anticipate that our results will be of interest to the reproductive biology community because an in-depth analysis of these MPs may identify potential new candidates in the context of human idiopathic infertilities.", "doi": "10.1021/acs.jproteome.7b00374", "pmid": "28891297", "labels": [], "xrefs": [], "notes": [], "created": "2018-12-05T12:07:55.856Z", "modified": "2026-09-23T10:59:37.889Z"}]}