{"entity": "researcher", "timestamp": "2026-09-23T18:38:58.698Z", "family": "Ankarcrona", "given": "Maria", "initials": "M", "orcid": "0000-0002-7022-3694", "affiliations": ["Department of Neurobiology, Care Sciences and Society, Division of Neurogeriatrics, Center for Alzheimer Research, Karolinska Institutet, Stockholm, Sweden."], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba3f69568d294081aa201e131eba2620.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba3f69568d294081aa201e131eba2620"}}, "publications": [{"entity": "publication", "iuid": "d776789418bb4a659c3b019d3142755b", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/d776789418bb4a659c3b019d3142755b.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/d776789418bb4a659c3b019d3142755b"}}, "title": "Simultaneous detection of membrane contact dynamics and associated Ca2+ signals by reversible chemogenetic reporters.", "authors": [{"family": "Garc\u00eda Casas", "given": "Paloma", "initials": "P"}, {"family": "Rossini", "given": "Michela", "initials": "M", "orcid": "0009-0008-9482-0371", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/567a75ae8a654e72b614c0df485cdd55.json"}}, {"family": "P\u00e5v\u00e9nius", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6730-2458", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/832383b2182344d995d2722d2bea9800.json"}}, {"family": "Saeed", "given": "Mezida", "initials": "M", "orcid": "0000-0003-1921-6015", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ecd266e5825e4864bd3cf1014b669334.json"}}, {"family": "Arnst", "given": "Nikita", "initials": "N"}, {"family": "Sonda", "given": "Sonia", "initials": "S"}, {"family": "Fernandes", "given": "T\u00e2nia", "initials": "T"}, {"family": "D'Arsi\u00e8", "given": "Irene", "initials": "I"}, {"family": "Bruzzone", "given": "Matteo", "initials": "M", "orcid": "0000-0001-7683-8107", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/536792d3769740f2ad2de212d1b5beb6.json"}}, {"family": "Berno", "given": "Valeria", "initials": "V"}, {"family": "Raimondi", "given": "Andrea", "initials": "A", "orcid": "0000-0002-4563-386X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ae862cd76c04029bd7a474fabe94a8a.json"}}, {"family": "Sassano", "given": "Maria Livia", "initials": "ML", "orcid": "0000-0002-2262-6915", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/988c7e2a244646039bc54282266826fd.json"}}, {"family": "Naia", "given": "Luana", "initials": "L", "orcid": "0000-0002-0886-4634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/594bb6850d604a64bc5ebe6752562231.json"}}, {"family": "Barbieri", "given": "Elisa", "initials": "E"}, {"family": "Sigismund", "given": "Sara", "initials": "S", "orcid": "0000-0002-1396-4453", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/e5bd219bf13e482ba1673099556a2529.json"}}, {"family": "Agostinis", "given": "Patrizia", "initials": "P", "orcid": "0000-0003-1314-2115", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/918570dfa3ed496ab16a118098754f53.json"}}, {"family": "Sturlese", "given": "Mattia", "initials": "M", "orcid": "0000-0003-3944-0313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3adcaee3edf4362947e3d560878d2d7.json"}}, {"family": "Niemeyer", "given": "Barbara A", "initials": "BA", "orcid": "0000-0002-6963-0575", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/104bf97a670a4889aa96cf52e436fc15.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H"}, {"family": "Ankarcrona", "given": "Maria", "initials": "M", "orcid": "0000-0002-7022-3694", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba3f69568d294081aa201e131eba2620.json"}}, {"family": "Gautier", "given": "Arnaud", "initials": "A", "orcid": "0000-0003-1017-9879", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51bda764b15b4171977acb3526699e0c.json"}}, {"family": "Pizzo", "given": "Paola", "initials": "P", "orcid": "0000-0001-6077-3265", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/af74153ebea445a985f0300ac30124fc.json"}}, {"family": "Filadi", "given": "Riccardo", "initials": "R", "orcid": "0000-0002-5871-2620", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b1abeb6ac7546029ad9fc9adb09e555.json"}}], "type": "journal article", "published": "2024-11-12", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "15", "issue": "1", "pages": "9775", "issn-l": "2041-1723"}, "abstract": "Membrane contact sites (MCSs) are hubs allowing various cell organelles to coordinate their activities. The dynamic nature of these sites and their small size hinder analysis by current imaging techniques. To overcome these limitations, we here design a series of reversible chemogenetic reporters incorporating improved, low-affinity variants of splitFAST, and study the dynamics of different MCSs at high spatiotemporal resolution, both in vitro and in vivo. We demonstrate that these versatile reporters suit different experimental setups well, allowing one to address challenging biological questions. Using these probes, we identify a pathway in which calcium (Ca2+) signalling dynamically regulates endoplasmic reticulum-mitochondria juxtaposition, characterizing the underlying mechanism. Finally, by integrating Ca2+-sensing capabilities into the splitFAST technology, we introduce PRINCESS (PRobe for INterorganelle Ca2+-Exchange Sites based on SplitFAST), a class of reporters to simultaneously detect MCSs and measure the associated Ca2+ dynamics using a single biosensor.", "doi": "10.1038/s41467-024-52985-0", "pmid": "39532847", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC11557831"}, {"db": "pii", "key": "10.1038/s41467-024-52985-0"}], "notes": [], "created": "2026-09-23T08:51:43.505Z", "modified": "2026-09-23T08:51:44.057Z"}, {"entity": "publication", "iuid": "bcaff66c0dd648eaa920454b34888c63", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/bcaff66c0dd648eaa920454b34888c63.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/bcaff66c0dd648eaa920454b34888c63"}}, "title": "Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters", "authors": [{"family": "Casas", "given": "Paloma Garc\u00eda", "initials": "PG"}, {"family": "Rossini", "given": "Michela", "initials": "M", "orcid": "0009-0008-9482-0371", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/567a75ae8a654e72b614c0df485cdd55.json"}}, {"family": "P\u00e5v\u00e9nius", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6730-2458", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/832383b2182344d995d2722d2bea9800.json"}}, {"family": "Saeed", "given": "Mezida", "initials": "M", "orcid": "0000-0003-1921-6015", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ecd266e5825e4864bd3cf1014b669334.json"}}, {"family": "Arnst", "given": "Nikita", "initials": "N"}, {"family": "Sonda", "given": "Sonia", "initials": "S"}, {"family": "Bruzzone", "given": "Matteo", "initials": "M", "orcid": "0000-0001-7683-8107", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/536792d3769740f2ad2de212d1b5beb6.json"}}, {"family": "Berno", "given": "Valeria", "initials": "V"}, {"family": "Raimondi", "given": "Andrea", "initials": "A", "orcid": "0000-0002-4563-386X", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1ae862cd76c04029bd7a474fabe94a8a.json"}}, {"family": "Sassano", "given": "Maria Livia", "initials": "ML", "orcid": "0000-0002-2262-6915", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/988c7e2a244646039bc54282266826fd.json"}}, {"family": "Naia", "given": "Luana", "initials": "L", "orcid": "0000-0002-0886-4634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/594bb6850d604a64bc5ebe6752562231.json"}}, {"family": "Agostinis", "given": "Patrizia", "initials": "P", "orcid": "0000-0003-1314-2115", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/918570dfa3ed496ab16a118098754f53.json"}}, {"family": "Sturlese", "given": "Mattia", "initials": "M", "orcid": "0000-0003-3944-0313", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/f3adcaee3edf4362947e3d560878d2d7.json"}}, {"family": "Niemeyer", "given": "Barbara A", "initials": "BA", "orcid": "0000-0002-6963-0575", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/104bf97a670a4889aa96cf52e436fc15.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Ankarcrona", "given": "Maria", "initials": "M", "orcid": "0000-0002-7022-3694", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba3f69568d294081aa201e131eba2620.json"}}, {"family": "Gautier", "given": "Arnaud", "initials": "A", "orcid": "0000-0003-1017-9879", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/51bda764b15b4171977acb3526699e0c.json"}}, {"family": "Pizzo", "given": "Paola", "initials": "P", "orcid": "0000-0001-6077-3265", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/af74153ebea445a985f0300ac30124fc.json"}}, {"family": "Filadi", "given": "Riccardo", "initials": "R", "orcid": "0000-0002-5871-2620", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/7b1abeb6ac7546029ad9fc9adb09e555.json"}}], "type": "posted-content", "published": "2023-12-28", "journal": {"issn-l": null}, "abstract": null, "doi": "10.1101/2023.12.28.573515", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-09-23T14:14:43.544Z", "modified": "2026-09-23T14:48:51.011Z"}, {"entity": "publication", "iuid": "5c451956fb8140fe954f54dc9e90a7fe", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5c451956fb8140fe954f54dc9e90a7fe.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5c451956fb8140fe954f54dc9e90a7fe"}}, "title": "Amyloid-\u03b2 accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolism.", "authors": [{"family": "Zy\u015bk", "given": "Marlena", "initials": "M"}, {"family": "Beretta", "given": "Chiara", "initials": "C"}, {"family": "Naia", "given": "Luana", "initials": "L", "orcid": "0000-0002-0886-4634", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/594bb6850d604a64bc5ebe6752562231.json"}}, {"family": "Dakhel", "given": "Abdulkhalek", "initials": "A"}, {"family": "P\u00e5v\u00e9nius", "given": "Linnea", "initials": "L", "orcid": "0000-0001-6730-2458", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/832383b2182344d995d2722d2bea9800.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/04321d9fb805493db538489927a42c8f.json"}}, {"family": "Lindskog", "given": "Maria", "initials": "M", "orcid": "0000-0001-9484-1983", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/de1f718eadcd41b7b3d5834bf34886b1.json"}}, {"family": "Ankarcrona", "given": "Maria", "initials": "M", "orcid": "0000-0002-7022-3694", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ba3f69568d294081aa201e131eba2620.json"}}, {"family": "Erlandsson", "given": "Anna", "initials": "A"}], "type": "journal article", "published": "2023-02-20", "journal": {"title": "J Neuroinflammation", "issn": "1742-2094", "volume": "20", "issue": "1", "pages": "43", "issn-l": "1742-2094"}, "abstract": "Astrocytes play a central role in maintaining brain energy metabolism, but are also tightly connected to the pathogenesis of Alzheimer's disease (AD). Our previous studies demonstrate that inflammatory astrocytes accumulate large amounts of aggregated amyloid-beta (A\u03b2). However, in which way these A\u03b2 deposits influence their energy production remain unclear.\n\nThe aim of the present study was to investigate how A\u03b2 pathology in astrocytes affects their mitochondria functionality and overall energy metabolism. For this purpose, human induced pluripotent cell (hiPSC)-derived astrocytes were exposed to sonicated A\u03b242 fibrils for 7 days and analyzed over time using different experimental approaches.\n\nOur results show that to maintain stable energy production, the astrocytes initially increased their mitochondrial fusion, but eventually the A\u03b2-mediated stress led to abnormal mitochondrial swelling and excessive fission. Moreover, we detected increased levels of phosphorylated DRP-1 in the A\u03b2-exposed astrocytes, which co-localized with lipid droplets. Analysis of ATP levels, when blocking certain stages of the energy pathways, indicated a metabolic shift to peroxisomal-based fatty acid \u03b2-oxidation and glycolysis.\n\nTaken together, our data conclude that A\u03b2 pathology profoundly affects human astrocytes and changes their entire energy metabolism, which could result in disturbed brain homeostasis and aggravated disease progression.", "doi": "10.1186/s12974-023-02722-z", "pmid": "36803838", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC9940442"}, {"db": "pii", "key": "10.1186/s12974-023-02722-z"}], "notes": [], "created": "2026-08-20T12:20:59.588Z", "modified": "2026-09-23T14:50:16.430Z"}]}