{"entity": "researcher", "timestamp": "2026-08-20T21:02:50.718Z", "family": "Wu", "given": "Yao\u2010Wen", "initials": "Y", "orcid": "0000-0002-2573-8736", "affiliations": ["SciLifeLab Department of Chemistry Ume\u00e5 University  90187 Ume\u00e5 Sweden", "Ume\u00e5 Centre for Microbial Research (UCMR) Ume\u00e5 University  90187 Ume\u00e5 Sweden"], "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad"}}, "publications": [{"entity": "publication", "iuid": "336851589e67468688a86631d1cb67b7", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/336851589e67468688a86631d1cb67b7.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/336851589e67468688a86631d1cb67b7"}}, "title": "Cover Feature: Visible\u2010Light\u2010Switchable Molecular Glues for Reversible Control of Protein Function (Chem. Eur. J. 15/2025)", "authors": [{"family": "Zhang", "given": "Jun", "initials": "J", "orcid": "0009-0005-1818-4009", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ee4c451f3b27452ba7960653e5ac2fb7.json"}}, {"family": "Herzog", "given": "Laura K", "initials": "LK", "orcid": "0000-0002-3322-7864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/777eef37d33d4c6a8e4bbf574601cc1e.json"}}, {"family": "Li", "given": "Shuang", "initials": "S"}, {"family": "Chen", "given": "Xi", "initials": "X"}, {"family": "Wu", "given": "Yao\u2010Wen", "initials": "Y", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}], "type": "journal-article", "published": "2025-03-12", "journal": {"title": "Chemistry", "issn": "0947-6539", "volume": "31", "issue": "15", "issn-l": null}, "abstract": null, "doi": "10.1002/chem.202581503", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:31:16.167Z", "modified": "2026-08-20T06:31:16.268Z"}, {"entity": "publication", "iuid": "4022d010846e424a90fca0dcba916789", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/4022d010846e424a90fca0dcba916789.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/4022d010846e424a90fca0dcba916789"}}, "title": "Visible-Light-Switchable Molecular Glues for Reversible Control of Protein Function.", "authors": [{"family": "Zhang", "given": "Jun", "initials": "J", "orcid": "0009-0005-1818-4009", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/ee4c451f3b27452ba7960653e5ac2fb7.json"}}, {"family": "Herzog", "given": "Laura K", "initials": "LK", "orcid": "0000-0002-3322-7864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/777eef37d33d4c6a8e4bbf574601cc1e.json"}}, {"family": "Li", "given": "Shuang", "initials": "S"}, {"family": "Chen", "given": "Xi", "initials": "X"}, {"family": "Wu", "given": "Yao-Wen", "initials": "YW", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}], "type": "journal article", "published": "2025-03-12", "journal": {"title": "Chemistry", "issn": "1521-3765", "volume": "31", "issue": "15", "pages": "e202403808", "issn-l": "0947-6539"}, "abstract": "Chemically induced dimerization/proximity (CID/CIP) systems controlled by chemical dimerizers (also known as molecular glues) provide valuable means for understanding and manipulating complex, dynamic biological systems. In this study, we present the development of versatile chemo-optogenetic systems utilizing azobenzene-based photoswitchable molecular glues (sMGs) for reversible protein dimerization controlled by visible light. These systems allow multiple cycles of light-induced dimerization, overcoming the limitations of irreversible photolysis in previous systems. Through optimizing photoswitch properties and linker strategies, we achieved efficient and reversible control using blue, green, and red light. We demonstrate that these systems enable rapid and reversible control of protein function in vitro and in cells. The findings represent a significant advancement in chemo-optogenetics, offering opportunities to expand applications requiring precise spatiotemporal regulation of dynamic biological processes.", "doi": "10.1002/chem.202403808", "pmid": "39805011", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:31:09.668Z", "modified": "2026-08-20T06:31:09.802Z"}, {"entity": "publication", "iuid": "803ec714a74043dbb8dcbf67e25ae5f2", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/803ec714a74043dbb8dcbf67e25ae5f2.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/803ec714a74043dbb8dcbf67e25ae5f2"}}, "title": "Modular Photoswitchable Molecular Glues for Chemo-Optogenetic Control of Protein Function in Living Cells.", "authors": [{"family": "Zhang", "given": "Jun", "initials": "J"}, {"family": "Herzog", "given": "Laura K", "initials": "LK", "orcid": "0000-0002-3322-7864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/777eef37d33d4c6a8e4bbf574601cc1e.json"}}, {"family": "Corkery", "given": "Dale P", "initials": "DP"}, {"family": "Lin", "given": "Tzu-Chen", "initials": "TC"}, {"family": "Klewer", "given": "Laura", "initials": "L"}, {"family": "Chen", "given": "Xi", "initials": "X"}, {"family": "Xin", "given": "Xiaoyi", "initials": "X"}, {"family": "Li", "given": "Yaozong", "initials": "Y"}, {"family": "Wu", "given": "Yao-Wen", "initials": "YW", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}], "type": "journal article", "published": "2025-02-10", "journal": {"title": "Angew. Chem. Int. Ed. Engl.", "issn": "1521-3773", "volume": "64", "issue": "7", "pages": "e202416456", "issn-l": "1433-7851"}, "abstract": "Optogenetic systems using photosensitive proteins and chemically induced dimerization/proximity (CID/CIP) approaches enabled by chemical dimerizers (also termed molecular glues), are powerful tools to elucidate the dynamics of biological systems and to dissect complex biological regulatory networks. Here, we report a versatile chemo-optogenetic system using modular, photoswitchable molecular glues (sMGs) that can undergo repeated cycles of optical control to switch protein function on and off. We use molecular dynamics (MD) simulations to rationally design the sMGs and further expand their scope by incorporating different photoswitches, resulting in sMGs with customizable properties. We demonstrate that this system can be used to reversibly control protein localization, organelle positioning, protein-fragment complementation as well as posttranslational protein levels by light with high spatiotemporal precision. This system enables sophisticated optical manipulation of cellular processes and thus opens up a new avenue for chemo-optogenetics.", "doi": "10.1002/anie.202416456", "pmid": "39777946", "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:30:00.143Z", "modified": "2026-08-20T06:30:00.237Z"}, {"entity": "publication", "iuid": "9b8c4b7cb693426f9a51603cc8ed62fc", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/9b8c4b7cb693426f9a51603cc8ed62fc.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/9b8c4b7cb693426f9a51603cc8ed62fc"}}, "title": "Modular Photoswitchable Molecular Glues for Chemo\u2010Optogenetic Control of Protein Function in Living Cells", "authors": [{"family": "Zhang", "given": "Jun", "initials": "J"}, {"family": "Herzog", "given": "Laura K", "initials": "LK", "orcid": "0000-0002-3322-7864", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/777eef37d33d4c6a8e4bbf574601cc1e.json"}}, {"family": "Corkery", "given": "Dale P", "initials": "DP"}, {"family": "Lin", "given": "Tzu\u2010Chen", "initials": "T"}, {"family": "Klewer", "given": "Laura", "initials": "L"}, {"family": "Chen", "given": "Xi", "initials": "X"}, {"family": "Xin", "given": "Xiaoyi", "initials": "X"}, {"family": "Li", "given": "Yaozong", "initials": "Y"}, {"family": "Wu", "given": "Yao\u2010Wen", "initials": "Y", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}], "type": "journal-article", "published": "2025-02-10", "journal": {"title": "Angewandte Chemie", "issn": "0044-8249", "volume": "137", "issue": "7", "issn-l": null}, "abstract": null, "doi": "10.1002/ange.202416456", "pmid": null, "labels": [], "xrefs": [], "notes": [], "created": "2026-08-20T06:29:50.004Z", "modified": "2026-08-20T06:29:50.101Z"}, {"entity": "publication", "iuid": "5371b7f2f25741168c44e0e79c3aa420", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/5371b7f2f25741168c44e0e79c3aa420.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/5371b7f2f25741168c44e0e79c3aa420"}}, "title": "ATG12-ATG5-TECPR1: an alternative E3-like complex utilized during the cellular response to lysosomal membrane damage.", "authors": [{"family": "Corkery", "given": "Dale P", "initials": "DP"}, {"family": "Wu", "given": "Yao-Wen", "initials": "YW", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}], "type": "journal article", "published": "2024-02-00", "journal": {"title": "Autophagy", "issn": "1554-8635", "volume": "20", "issue": "2", "pages": "443-444", "issn-l": "1554-8627"}, "abstract": "ATG16L1 is an essential component of the Atg8-family protein conjugation machinery, providing membrane targeting for the ATG12-ATG5 conjugate. Recently, we identified an alternative E3-like complex that functions independently of ATG16L1. This complex utilizes the autophagosome-lysosome tethering factor TECPR1 for membrane targeting. TECPR1 is recruited to damaged lysosomal membranes via a direct interaction with sphingomyelin. At the damaged membrane, TECPR1 assembles into an E3-like complex with ATG12-ATG5 to regulate unconventional LC3 lipidation and promote efficient lysosomal repair.", "doi": "10.1080/15548627.2023.2267414", "pmid": "37872727", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10813570"}], "notes": [], "created": "2026-08-20T09:36:54.584Z", "modified": "2026-08-20T09:36:54.627Z"}, {"entity": "publication", "iuid": "ce409bdcc45548f2931a8d734210082a", "links": {"self": {"href": "https://publications-affiliated.scilifelab.se/publication/ce409bdcc45548f2931a8d734210082a.json"}, "display": {"href": "https://publications-affiliated.scilifelab.se/publication/ce409bdcc45548f2931a8d734210082a"}}, "title": "Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles.", "authors": [{"family": "Nanda", "given": "Suchet", "initials": "S", "orcid": "0000-0001-9863-8069", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/6835f52814b549aa92331cef548eb559.json"}}, {"family": "Calderon", "given": "Abram", "initials": "A"}, {"family": "Sachan", "given": "Arya", "initials": "A", "orcid": "0009-0006-3631-9648", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/3e69567686454dc18698f4dd80ea75d7.json"}}, {"family": "Duong", "given": "Thanh-Thuy", "initials": "TT"}, {"family": "Koch", "given": "Johannes", "initials": "J"}, {"family": "Xin", "given": "Xiaoyi", "initials": "X"}, {"family": "Solouk-Stahlberg", "given": "Djamschid", "initials": "D"}, {"family": "Wu", "given": "Yao-Wen", "initials": "YW", "orcid": "0000-0002-2573-8736", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/b915c6698bf44f3a88a880af261eb0ad.json"}}, {"family": "Nalbant", "given": "Perihan", "initials": "P", "orcid": "0000-0002-5649-5933", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/9cbf7f82e1c345ce8c25a6b0f8b62805.json"}}, {"family": "Dehmelt", "given": "Leif", "initials": "L", "orcid": "0000-0002-6559-6496", "researcher": {"href": "https://publications-affiliated.scilifelab.se/researcher/1c30fde517264f868a1eb398e5ada7ad.json"}}], "type": "journal article", "published": "2023-12-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "14", "issue": "1", "pages": "8356", "issn-l": "2041-1723"}, "abstract": "Rho GTPases play a key role in the spatio-temporal coordination of cytoskeletal dynamics during cell migration. Here, we directly investigate crosstalk between the major Rho GTPases Rho, Rac and Cdc42 by combining rapid activity perturbation with activity measurements in mammalian cells. These studies reveal that Rac stimulates Rho activity. Direct measurement of spatio-temporal activity patterns show that Rac activity is tightly and precisely coupled to local cell protrusions, followed by Rho activation during retraction. Furthermore, we find that the Rho-activating Lbc-type GEFs Arhgef11 and Arhgef12 are enriched at transient cell protrusions and retractions and recruited to the plasma membrane by active Rac. In addition, their depletion reduces activity crosstalk, cell protrusion-retraction dynamics and migration distance and increases migration directionality. Thus, our study shows that Arhgef11 and Arhgef12 facilitate exploratory cell migration by coordinating cell protrusion and retraction by coupling the activity of the associated regulators Rac and Rho.", "doi": "10.1038/s41467-023-43875-y", "pmid": "38102112", "labels": [], "xrefs": [{"db": "pmc", "key": "PMC10724141"}, {"db": "pii", "key": "10.1038/s41467-023-43875-y"}], "notes": [], "created": "2026-08-20T08:53:04.437Z", "modified": "2026-08-20T08:53:04.648Z"}]}